US4902630A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fluorescence polarization immunoassy and reagents for measurement of c-reactive protein
Assignee: Abbott Laboratories
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Filing: 1988-07-14
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Granted: 1990-02-20
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Expires: 2008-07-14
Abstract
Abstract This disclosure relates to a fluorescence polarization immunoassay method for determining C-reactive protein in liquids, especially in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. This disclosure also relates to novel reagents useful in such fluorescence polarization immunoassays.
Inventors
- 👤 Larry G. Bennett
- 👤 Enrico G. Chiapetta
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 11:36
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/582; G01N33/53; G01N33/536; G01N33/542; G01N33/68; G01N33/6893; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/815
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
101 words
A method for determining the presence of concentration of C-reactive protein in a liquid sample of biological fluid, comprising intermixing the a sample of said biological fluid a fluorescent tracer comprising a conjugate of C-reactive protein and a fluorescent moiety, so as to be specifically recognizable by a common antibody or receptor binding site with a solution of an antibody capable of specifically recognizing the C-reactive protein in the sample and the tracer; and determining the amount of the tracer which becomes bound to the antibody by fluorescence polarization as a measure of the amount of C-reactive protein in the sample.
Claim 2
Independent Claim
101 words
A method for determining the presence of concentration of C-reactive protein in a liquid sample of biological fluid, comprising intermixing the a sample of said biological fluid a fluorescent tracer comprising a conjugate of C-reactive protein and a fluorescent moiety, so as to be specifically recognizable by a common antibody or receptor binding site with a solution of an antibody capable of specifically recognizing the C-reactive protein in the sample and the tracer; and determining the amount of the tracer which becomes bound to the antibody by fluorescence polarization as a measure of the amount of C-reactive protein in the sample.
Claim 3
Dependent Claim
24 words
The method of claim 1 where said step (a) is conducted at a pH sufficient to maintain said fluorescent tracer in an ionized state.
Claim 4
Dependent Claim
12 words
The method of claim 1 where said fluorescent moiety is 5(4,6-dichlorotriazin-2-yl)-amino fluorescein.
Claim 5
Dependent Claim
36 words
A compound useful in a fluorescence polarization assay for determining the presence or amount of a C-reactive protein in a liquid sample of a biological fluid, which compound consisting of C-reactive protein conjugated to 5(4,6-dichlorotriazin-2-yl)amino fluorescein.
Claim 6
Dependent Claim
36 words
A compound useful in a fluorescence polarization assay for determining the presence or amount of a C-reactive protein in a liquid sample of a biological fluid, which compound consisting of C-reactive protein conjugated to 5(4,6-dichlorotriazin-2-yl)amino fluorescein.
References Cited (Backward Citations)
GB2081257A
Cited
US4447545A
Cited
US4492762A
Cited
US4492762B1
Cited
US4504585A
Cited
US4681859A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-02-19 | 1993-08-21 | 1994-02-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-02-19 | 1997-08-21 | 1998-02-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-02-19 | 2001-08-21 | 2002-02-20 | $7700.00 | Window Closed / Lapsed |