US4895806A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Device for liquid chromatography or immobilized enzyme reaction
Assignee: Merck Millipore Ltd
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Filing: 1989-06-13
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Granted: 1990-01-23
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Expires: 2009-06-13
Abstract
Abstract A device for separating molecular components from liquids. A multi-layer microporous membrane assembly is located within the housing (1) with edges of each membrane within the assembly lying adjacent to a barrier. Means (5,6) are provided for substantially preventing flow between the membrane edges and the barrier, so that flow is through the membranes in series. Inlet means (2) direct incoming liquid on to a first surface of the membrane assembly, and outlet means (3) gather and direct outgoing liquid from a second surface of the membrane assembly. The membrane material of the assembly has a ligand or an immobilized enzyme bound thereto for effecting chromatographic separation or an immobilized enzyme reaction.
Inventors
- 👤 Minh S. Le
- 👤 James A. Sanderson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 05:10
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3857; G; G01; G01N; G01N30/00; G01N30/02; G01N30/26; G01N30/38; G01N2030/386; G01N30/50; G01N30/52; G01N2030/524; G01N2030/527; G01N30/60; G01N30/6004; G01N30/6017
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
120 words
A device for the separation of molecular components from liquids containing the same, comprising a housing, a multi-layer microporous membrane assembly within the housing wherein a plurality of membrane layers are disposed in face-to-face relation with edges of each membrane within the assembly lying adjacent to a barrier, means for substantially preventing flow between the membrane edges and the barrier, inlet means for directing incoming liquid on to a first surface of the membrane assembly, and outlet means for gathering and directing outgoing liquid from a second surface of the membrane assembly, wherein the flow of liquid is successively through the plurality of membrane layers, the membrane material of the assembly having bound thereto a ligand or an immobilised enzyme.
Claim 2
Independent Claim
120 words
A device for the separation of molecular components from liquids containing the same, comprising a housing, a multi-layer microporous membrane assembly within the housing wherein a plurality of membrane layers are disposed in face-to-face relation with edges of each membrane within the assembly lying adjacent to a barrier, means for substantially preventing flow between the membrane edges and the barrier, inlet means for directing incoming liquid on to a first surface of the membrane assembly, and outlet means for gathering and directing outgoing liquid from a second surface of the membrane assembly, wherein the flow of liquid is successively through the plurality of membrane layers, the membrane material of the assembly having bound thereto a ligand or an immobilised enzyme.
Claim 3
Dependent Claim
17 words
A device according to claim 1 in which the membrane assembly comprises at least 50 membrane layers.
Claim 4
Dependent Claim
118 words
A device according to claim 1 in which the barrier is formed by side wall means of the housing, and the means for substantially preventing flow between the membrane edges and the side wall means comprise first resilient sealing means between a first end wall of the housing and the periphery of the first membrane of the stack, second resilient sealing means between a second end wall of the housing and the periphery of the last membrane of the stack, and a plurality of resilient sealing washers interposed between the periphery of two adjacent membranes at intervals through the stack, the sealing means and sealing washers each having a circumferential surface making contact with the side wall means.
Claim 5
Dependent Claim
23 words
A device according to claim 3 in which sealing washers are present in the stack at intervals of from 5 to 10 membranes.
Claim 6
Dependent Claim
31 words
A device according to claim 3 in which the side wall means is a circular cylindrical wall, the membranes are circular discs and the sealing means and washers are each annular.
Claim 7
Dependent Claim
59 words
A device according to claim 1 in which the edges of the membranes lie adjacent to side wall means of the housing, and the means for substantially preventing flow between the membrane edges and the side wall means comprises a sealant composition bonded to the side wall means and to the peripheral regions of the membranes forming the stack.
Claim 8
Dependent Claim
40 words
A device according to claim 1 in which the inlet means includes flow distribution means effective to distribute incoming liquid over a substantial area of the housing before the liquid makes contact with the first surface of the membrane assembly.
Claim 9
Independent Claim
109 words
A device for the separation of molecular components from liquids containing the same, comprising a housing, a multi-layer microporous membrane assembly within the housing comprising a membrane sheet wound around a porous core to form a plurality of layers with the edges of each layer lying adjacent to a barrier, means for substantially preventing flow between the membrane edges and the barrier, inlet means for directing incoming liquid on to a first surface of the membrane assembly, and outlet means for gathering and directing outgoing liquid from a second surface of the membrane assembly, the membrane material of the assembly having bound thereto a ligand or an immobilised enzyme.
Claim 10
Independent Claim
109 words
A device for the separation of molecular components from liquids containing the same, comprising a housing, a multi-layer microporous membrane assembly within the housing comprising a membrane sheet wound around a porous core to form a plurality of layers with the edges of each layer lying adjacent to a barrier, means for substantially preventing flow between the membrane edges and the barrier, inlet means for directing incoming liquid on to a first surface of the membrane assembly, and outlet means for gathering and directing outgoing liquid from a second surface of the membrane assembly, the membrane material of the assembly having bound thereto a ligand or an immobilised enzyme.
Claim 11
Dependent Claim
49 words
A device according to claim 8 in which the wound edges of the membrane sheet abut against a barrier formed by sealant composition bonded to the edge regions of the membrane sheet, the composition also forming the means for substantially preventing flow between the membrane edges and the barrier.
Claim 12
Independent Claim
166 words
A device for the separation of molecular components from liquids containing the same, comprising a housing, a multi-layer microporous membrane assembly within the housing with edges of each membrane within the assembly lying adjacent to a barrier, means for substantially preventing flow between the membrane edges and the barrier, inlet means for directing incoming liquid on to a first surface of the membrane assembly including a flow distribution means effective to distribute incoming liquid over a substantial area of the housing before the liquid makes contact with the first surface of the membrane assembly comprising a porous disc overlying the first surface of the membrane assembly, said disc having a solid, non-porous section immediately aligned with an inlet opening, and a distributor arrangement for distributing incoming liquid over the surface of the porous disc, and outlet means for gathering and directing outgoing liquid from a second surface of the membrane assembly, the membrane material of the assembly having bound thereto a ligand or an immobilised enzyme.
Claim 13
Independent Claim
166 words
A device for the separation of molecular components from liquids containing the same, comprising a housing, a multi-layer microporous membrane assembly within the housing with edges of each membrane within the assembly lying adjacent to a barrier, means for substantially preventing flow between the membrane edges and the barrier, inlet means for directing incoming liquid on to a first surface of the membrane assembly including a flow distribution means effective to distribute incoming liquid over a substantial area of the housing before the liquid makes contact with the first surface of the membrane assembly comprising a porous disc overlying the first surface of the membrane assembly, said disc having a solid, non-porous section immediately aligned with an inlet opening, and a distributor arrangement for distributing incoming liquid over the surface of the porous disc, and outlet means for gathering and directing outgoing liquid from a second surface of the membrane assembly, the membrane material of the assembly having bound thereto a ligand or an immobilised enzyme.
Claim 14
Dependent Claim
53 words
A device according to claim 10 in which the inlet opening is formed in a cap forming an end wall of the housing, an inner surface of the cap lies in contact with the porous disc, and the distributor arrangement comprises a series of channels formed in the inner surface of the cap.
Claim 15
Dependent Claim
26 words
A device according to claim 11 in which the total void volume in the channels does not exceed 1% of the volume of the membrane assembly.
Claim 16
Dependent Claim
30 words
. A device according to claim 11 in which the flow regime throughout the channel system is substantially constant with a Reynolds number not exceeding 1500 under normal operating conditions.
Claim 17
Dependent Claim
36 words
A device according to claim 11 in which the cap is circular, the inlet is at the centre of the cap, and the channels form a dendritic arrangement radiating outwardly from the centre of the cap.
Claim 18
Dependent Claim
40 words
A device according to claim 14 in which the sum of the shortest paths from any point on the inner surface of the cap to the two nearest channels does not exceed one tenth the diameter of the membrane assembly.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-01-22 | 1993-07-24 | 1994-01-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-01-22 | 1997-07-24 | 1998-01-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-01-22 | 2001-07-24 | 2002-01-23 | $7700.00 | Window Closed / Lapsed |