Patent US1819482A successfully refreshed from Google Patents.
US4517291A
Expired / Lapsed
US
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Biological detection process using polymer-coated electrodes
Assignee: Dade Behring Inc
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Filing: 1983-08-15
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Granted: 1985-05-14
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Expires: 2003-08-15
Abstract
Abstract A method is described for the amperometric determination of the concentration in a biological sample of a constituent which is a substrate for an oxidase enzyme by (1) catalyzing the substrate with the enzyme, thereby consuming O 2 and producing H 2 O 2 and (2) measuring the O 2 consumption or H 2 O 2 production by means of an inert metal indicator electrode intimately coated with a thin film of a perfluorosulfonic acid polymer. A reaction chamber is described which provides means for contacting the sample with the enzyme and means for making amperometric measurements utilizing inert metal indicator electrodes coated with a thin film of a perfluorosulfonic acid polymer.
Inventors
- 👤 James L. Seago
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 17:45
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; C12M; C12M41/00; C12M41/30; C12M41/36; Y; Y10; Y10S; Y10S435/00; Y10S435/817; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
127 words
In a method for the amperometric determination of the concentration of a constituent of interest in a biological sample, said method comprising: (1) contacting said constituent with an oxidase enzyme for which the constituent is a substrate, thereby producing a change in the concentration of an electroactive substance in said sample; (2) contacting said sample containing said electroactive substance with an inert metal indicator electrode; (3) measuring the current flow between said indicator electrode and a counter electrode at a preselected potential difference between said electrodes; and (4) relating the current to the concentration of the substrate initially present in the biological sample; the improvement comprising: using as the inert metal indicator electrode a noble metal having intimately coated thereon a thin film of perfluorosulfonic acid polymer.
Claim 2
Dependent Claim
127 words
In a method for the amperometric determination of the concentration of a constituent of interest in a biological sample, said method comprising: (1) contacting said constituent with an oxidase enzyme for which the constituent is a substrate, thereby producing a change in the concentration of an electroactive substance in said sample; (2) contacting said sample containing said electroactive substance with an inert metal indicator electrode; (3) measuring the current flow between said indicator electrode and a counter electrode at a preselected potential difference between said electrodes; and (4) relating the current to the concentration of the substrate initially present in the biological sample; the improvement comprising: using as the inert metal indicator electrode a noble metal having intimately coated thereon a thin film of perfluorosulfonic acid polymer.
Claim 3
Dependent Claim
19 words
The method of claim 1 wherein the noble metal is selected from the group consisting of platinum and gold.
Claim 4
Dependent Claim
11 words
The method of claim 2 wherein the noble metal is platinum.
Claim 5
Dependent Claim
19 words
The method of claim 1 wherein the constituent of interest is glucose and the oxidase enzyme is glucose oxidase.
Claim 6
Dependent Claim
11 words
The method of claim 4 wherein the noble metal is platinum.
Claim 7
Dependent Claim
19 words
The method of claim 1 wherein the perfluorosulfonic acid polymer has an equivalent weight of at least about 900.
Claim 8
Dependent Claim
17 words
The method of claim 6 wherein the perfluorosulfonic acid polymer has an equivalent weight of about 1100.
Claim 9
Dependent Claim
15 words
The method of claim 1 wherein the thin film is less than about 0.005 inch.
Claim 10
Dependent Claim
13 words
The method of claim 1 wherein the thin film is about 0.001 inch.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-05-13 | 1988-11-12 | 1989-05-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-05-13 | 1992-11-12 | 1993-05-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-05-13 | 1996-11-12 | 1997-05-14 | $7700.00 | Window Closed / Lapsed |