US4894338A
Expired / Lapsed
US
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Yeast strains producing cellulolytic enzymes and methods and means for constructing them
Assignee: AB Enzymes Oy
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Filing: 1985-04-12
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Granted: 1990-01-16
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Expires: 2005-04-12
Abstract
Abstract The full length DNA sequence encoding the mature cellobiohydrolase II(CHBII), as well as the DNA sequence encoding the CBHII signal sequence, have been isolated and cloned from the fungus Trichoderma reesei. Recombinant vectors comprising these sequences, and yeast hosts transformed with such vectors are disclosed. Also disclosed are methods for constructing the vectors and hosts according to the invention, as well as methods for expressing the disclosed sequences. CBHII is involved in hydrolysis of cellulose.
Inventors
- 👤 Jonathan Knowles
- 👤 Merja Penttila
- 👤 Tuula Teeri
- 👤 Helena Nevalainen
- 👤 Irma Salovuori
- 👤 Paivi Lehtovaara-Helenius
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 13:32
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2437; C12N15/00; C12N15/09; C12N15/11; C12N15/62; C12N15/625; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C12Y; C12Y302/00; C12Y302/01; C12Y302/01091; C07; C07K; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/034; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/945
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
42 words
A substantially pure DNA sequence or its single or multiple base substitutions, deletions, insertions or inversions, encoding an amino acid sequence comprising the mature cellobiohydrolase II protein amino acid sequence shown in FIG. 5, or a portion thereof having cellobiohydrolase II activity.
Claim 2
Dependent Claim
42 words
A substantially pure DNA sequence or its single or multiple base substitutions, deletions, insertions or inversions, encoding an amino acid sequence comprising the mature cellobiohydrolase II protein amino acid sequence shown in FIG. 5, or a portion thereof having cellobiohydrolase II activity.
Claim 3
Dependent Claim
36 words
The DNA sequence of claim 1, additionally comprising a DNA. sequence encoding an amino acid sequence comprising the cellobiohydrolase II signal sequence shown in FIG. 5, or a portion thereof having cellobiohydrolase II signal sequence activity.
Claim 4
Dependent Claim
41 words
A substantially pure DNA sequence comprising the cDNA sequence shown in FIG. 5 encoding an amino acid sequence comprising the mature cellobiohydrolase II protein amino acid sequence, or a portion thereof which encodes an amino acid sequence having cellobiohydrolase II activity.
Claim 5
Dependent Claim
41 words
A substantially pure DNA sequence comprising the cDNA sequence shown in FIG. 5 encoding an amino acid sequence comprising the mature cellobiohydrolase II protein amino acid sequence, or a portion thereof which encodes an amino acid sequence having cellobiohydrolase II activity.
Claim 6
Dependent Claim
43 words
The DNA sequence of claim 3, additionally comprising the cDNA sequence shown in FIG. 5 which encodes an amino acid sequence comprising the cellobiohydrolase II signal sequence, or a portion thereof which encodes an amino acid sequence having cellobiohydrolase II signal sequence activity.
Claim 7
Dependent Claim
20 words
The DNA sequence of claim 3, wherein said cDNA is a cDNA copy of Trichoderma reesei mRNA encoding cellobiohydrolase II.
Claim 8
Dependent Claim
40 words
The DNA sequence of claim 4, wherein said cDNA sequence shown in FIG. 5 which encodes an amino acid sequence comprising the cellobiohydrolase II signal sequence, is a cDNA copy of Trichoderma reesei mRNA encoding the cellobiohydrolase II signal sequence.
Claim 9
Dependent Claim
30 words
A substantially pure DNA sequence encoding an amino acid sequence comprising the cellobiohydrolase II signal sequence shown in FIG. 5, or a portion thereof having cellobiohydrolase II signal sequence activity.
Claim 10
Dependent Claim
30 words
A substantially pure DNA sequence encoding an amino acid sequence comprising the cellobiohydrolase II signal sequence shown in FIG. 5, or a portion thereof having cellobiohydrolase II signal sequence activity.
Claim 11
Dependent Claim
20 words
A recombinant DNA vector comprising the DNA sequence of any of claims 1, 2, 3, 4, 5, 6, or 7.
Claim 12
Dependent Claim
10 words
A yeast host transformed with the vector of claim 8.
Claim 13
Dependent Claim
14 words
The yeast host of claim 9, wherein said host is of the genus Saccharomyces.
Claim 14
Dependent Claim
13 words
The yeast host of claim 10, wherein said Saccharomyces host is S. cerevisiae.
Claim 15
Independent Claim
31 words
A method for constructing a recombinant DNA vector, comprising ligating into a suitable plasmid or phage the DNA sequence of any of claims 1, 2, 3, 4, 5, 6, or 7.
Claim 16
Independent Claim
45 words
A method for DNA expression, comprising transforming a yeast host with a recombinant DNA vector comprising the DNA sequence of any of claims 1, 2, 3, 4, 5, 6, or 7, and culturing said host under conditions wherein said DNA sequence is transcribed and translated.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-01-15 | 1993-07-17 | 1994-01-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-01-15 | 1997-07-17 | 1998-01-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-01-15 | 2001-07-17 | 2002-01-16 | $7700.00 | Window Closed / Lapsed |