Patent US4890925A successfully refreshed from Google Patents.
US4890925A
Expired / Lapsed
US
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Method and apparatus for detecting particular particulate substance
Assignee: Hitachi Ltd
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Filing: 1987-08-10
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Granted: 1990-01-02
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Expires: 2007-08-10
Abstract
Abstract By use of an excitation beam having a wavelength which is equal to, or close to, the sizes of particular particulate substances as the object of analysis, the particulate substances such as an immunoreaction product between an antigen and an antibody or between the antigen and a material supporting the antibody thereon are detected selectively. Very small particles having specific sizes such as rheumatuid factors, cancer specific antigens can be detected with high sensitivity.
Inventors
- 👤 Takehiko Kitamori
- 👤 Kazuo Yasuda
- 👤 Yasushi Nomura
- 👤 Kazumichi Suzuki
- 👤 Haruo Fujimori
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 10:53
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/1702; G01N15/00; G01N15/06; G01N21/75; G01N33/00; G01N33/48; G01N33/50; G01N33/53
Claims Analysis
Total Claims: 73
Claim 1
Independent Claim
56 words
A method of detecting a particular particulate substance comprising the steps of: adjusting or selecting a wavelength of a ray of light to be substantially equal to the size of the particular particulate substance; radiating the ray of light to the particulate substance; and measuring the magnitude of a photoacoustic signal generated from the particulate substance.
Claim 2
Independent Claim
56 words
A method of detecting a particular particulate substance comprising the steps of: adjusting or selecting a wavelength of a ray of light to be substantially equal to the size of the particular particulate substance; radiating the ray of light to the particulate substance; and measuring the magnitude of a photoacoustic signal generated from the particulate substance.
Claim 3
Dependent Claim
31 words
A method according to claim 1, wherein said particulate substance is an immunoreaction product between an antigen and an antibody or between the antigen and a material supporting the antibody thereon.
Claim 4
Dependent Claim
39 words
A method according to claim 2, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range capable of embracing the particulate size distribution of the particulate substance.
Claim 5
Dependent Claim
33 words
A method according to claim 2, wherein the support material for tha antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 6
Dependent Claim
31 words
A method according to claim 1, wherein said particulate substance is an immunoreaction product between an antibody and an antigen or between the antibody and a material supporting the antigen thereon.
Claim 7
Dependent Claim
39 words
A method according to claim 5, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range capable of embracing the particulate size distribution of the particulate substance.
Claim 8
Dependent Claim
33 words
A method according to claim 5, wherein the support material for said antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 9
Dependent Claim
39 words
A method according to claim 1, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light with a wavelength range capable of embracing the particulate size distribution of the particulate substance.
Claim 10
Independent Claim
82 words
A method of detecting a particular particulate substance comprising the steps of: adjusting or selecting a wavelength of a ray of light to be substantially equal to the size of the particular particulate substance; radiating the ray of light to the particulate substance; measuring the magnitude of a photoacoustic signal generated from the particular substance; and determining the concentration of the particulate substance by use of a relationship between the magnitude of photoacoustic signals and the concentration of a particular particulate substance.
Claim 11
Independent Claim
82 words
A method of detecting a particular particulate substance comprising the steps of: adjusting or selecting a wavelength of a ray of light to be substantially equal to the size of the particular particulate substance; radiating the ray of light to the particulate substance; measuring the magnitude of a photoacoustic signal generated from the particular substance; and determining the concentration of the particulate substance by use of a relationship between the magnitude of photoacoustic signals and the concentration of a particular particulate substance.
Claim 12
Dependent Claim
31 words
A method according to claim 9, wherein the particular substance is an immunoreaction product between an antigen and an antibody or between the antigen and a material supporting the antibody thereon.
Claim 13
Dependent Claim
39 words
A method according to claim 10, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of the particulate substance.
Claim 14
Dependent Claim
33 words
A method according to claim 10, wherein the support material for the antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 15
Dependent Claim
31 words
A method according to claim 9, wherein the particulate substance is an immunoreaction product between an antibody and an antigen or between the antibody and a material supporting the antigen thereon.
Claim 16
Dependent Claim
39 words
A method according to claim 13, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of the particulate substance.
Claim 17
Dependent Claim
33 words
A method according to claim 13, wherein the support material for the antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 18
Dependent Claim
39 words
A method according to claim 9, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of the particulate substance.
Claim 19
Independent Claim
50 words
A method of detecting a particular particulate substance comprising the steps of: radiating a ray of light of a wavelength selected to be substantially equal to the size of the particular particulate substance to the particulate substance; and measuring the magnitude of a photoacoustic signal generated from the particulate substance.
Claim 20
Independent Claim
50 words
A method of detecting a particular particulate substance comprising the steps of: radiating a ray of light of a wavelength selected to be substantially equal to the size of the particular particulate substance to the particulate substance; and measuring the magnitude of a photoacoustic signal generated from the particulate substance.
Claim 21
Dependent Claim
31 words
A method according to claim 17, wherein the particulate substance is an immunoreaction product between the antigen and an antibody or between the antigen and a material supporting the antibody thereon.
Claim 22
Independent Claim
39 words
A method according to clam 18, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of the particulate substance.
Claim 23
Independent Claim
39 words
A method according to clam 18, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of the particulate substance.
Claim 24
Dependent Claim
33 words
A method according to claim 18, wherein the support material for the antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 25
Dependent Claim
31 words
A method according to claim 17, wherein the particulate substance is an immunoreaction product between an antibody and an antigen or between the antibody and a material supporting the antigen thereon.
Claim 26
Dependent Claim
38 words
A method according to claim 21, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range of embracing the particle size distribution of the particulate substance.
Claim 27
Dependent Claim
33 words
A method according to claim 21, wherein the support material for the antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 28
Dependent Claim
39 words
A method according to claim 17, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of the particulate substance.
Claim 29
Independent Claim
77 words
A method of detecting a particular particulate substance comprising the steps of: radiating a ray of a light of wavelength selected to be substantially equal to the diameter of the particular particulate substance to the particulate substance, and measuring the magnitude of a photoacoustic signal generated from the particulate substance; and determining the concentration of the particulate substance by use of a relationship between the magnitude of photoacoustic signals and the concentration of a particular particulate substance.
Claim 30
Independent Claim
77 words
A method of detecting a particular particulate substance comprising the steps of: radiating a ray of a light of wavelength selected to be substantially equal to the diameter of the particular particulate substance to the particulate substance, and measuring the magnitude of a photoacoustic signal generated from the particulate substance; and determining the concentration of the particulate substance by use of a relationship between the magnitude of photoacoustic signals and the concentration of a particular particulate substance.
Claim 31
Dependent Claim
31 words
A method according to claim 25, wherein the particulate substance is an immunoreaction product between an antigen and an antibody or between the antigen and a material supporting the antibody thereon.
Claim 32
Dependent Claim
39 words
A method according to claim 26, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of the particulate substance.
Claim 33
Dependent Claim
33 words
A method according to claim 26, wherein the support material for the antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 34
Dependent Claim
31 words
A method according to claim 25, wherein the particulate substance is an immunoreaction product between an antibody and an antigen or between the antibody and a material supporting the antigen thereon.
Claim 35
Dependent Claim
39 words
A method according to claim 29, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of the particulate substance.
Claim 36
Dependent Claim
33 words
A method according to claim 29, wherein the support material for the antibody ia a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 37
Dependent Claim
39 words
A method according to claim 25, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of the particulate substance.
Claim 38
Independent Claim
56 words
A method of detecting a particular particulate substance comprising the steps of: adjusting or selecting the size of the particular particulate substance to be substantially equal to the wavelength of a ray of light; radiating the ray of light to the particular substance; and measuring the magnitude of a photoacoustic signal generated from the particulate substance.
Claim 39
Independent Claim
56 words
A method of detecting a particular particulate substance comprising the steps of: adjusting or selecting the size of the particular particulate substance to be substantially equal to the wavelength of a ray of light; radiating the ray of light to the particular substance; and measuring the magnitude of a photoacoustic signal generated from the particulate substance.
Claim 40
Dependent Claim
31 words
A method according to claim 33, wherein the particulate substance is an immunoreaction product between an antigen and an antibody or between the antigen and an material supporting the antibody thereon.
Claim 41
Dependent Claim
39 words
A method according to claim 34, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of the particulate substance.
Claim 42
Dependent Claim
33 words
A method according to claim 34, wherein the support material for the antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 43
Dependent Claim
31 words
A method according to claim 33, wherein the particulate substance is an immunoreaction product between an antibody and an antigen or between the antibody and a material supporting the antigen thereon.
Claim 44
Dependent Claim
39 words
A method according to claim 37, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of the particulate substance.
Claim 45
Dependent Claim
33 words
A method according to claim 37, wherein the support material for the antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 46
Dependent Claim
39 words
A method according to claim 33, wherein the ray of light to be radiated to the particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of the particulate substance.
Claim 47
Independent Claim
91 words
An apparatus for detecting a particular particulate substance comprising: a light source; wavelength converter means for converting a wavelength of a ray of light provided from said light source to a wavelength selected to be substantially equal to the size of said particular particulate substance; a cell into which said particular substance is charged and which receives the ray of light whose wavelength is converted, and outputs a photoacoustic signal; a data processor for inputting said photoacoustic signal and discriminating and outputting a photoacoustic signal generated from said particular particulate substance.
Claim 48
Independent Claim
91 words
An apparatus for detecting a particular particulate substance comprising: a light source; wavelength converter means for converting a wavelength of a ray of light provided from said light source to a wavelength selected to be substantially equal to the size of said particular particulate substance; a cell into which said particular substance is charged and which receives the ray of light whose wavelength is converted, and outputs a photoacoustic signal; a data processor for inputting said photoacoustic signal and discriminating and outputting a photoacoustic signal generated from said particular particulate substance.
Claim 49
Dependent Claim
31 words
An apparatus according to claim 41, wherein said particulate substance is an immunoreaction product between an antigen and an antibody or between the antigen and a material supporting the antibody thereon.
Claim 50
Dependent Claim
39 words
An apparatus according to claim 42, wherein the ray of light to be radiated to said particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of said particulate substance.
Claim 51
Dependent Claim
33 words
An apparatus according to claim 42, wherein said support material for said antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 52
Dependent Claim
31 words
An apparatus according to claim 41, wherein said particulate substance is an immunoreaction product between an antibody and an antigen or between the antibody and a material supporting the antigen thereon.
Claim 53
Dependent Claim
39 words
An apparatus according to claim 45, wherein the ray of light to be radiated to said particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of said particulate substance.
Claim 54
Dependent Claim
33 words
An apparatus according to claim 45, wherein said support material for said antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 55
Dependent Claim
39 words
An apparatus according to claim 41, wherein the ray of light to be radiated to said particulate substance is a ray of continuous light within a wavelength range capable of embracing the particulate size distribution of said particulate substance.
Claim 56
Independent Claim
127 words
An apparatus for detecting a particular particulate substance comprising: a light source; wavelength converter means for converting a wavelength of a ray of light provided from said light source to a wavelength selected to be substantially equal to the size of said particular particulate substance; a cell into which said particulate substance as the object of analysis is charged and which receives the ray of light whose wavelength is converted, and outputs a photoacoustic signal; a data processor for inputting said photoacoustic signal and determining the concentration of said particulate substance by use of a relationship between the magnitude of photoacoustic signals and the concentration of a particular particulate substance that is previously stored therein; and a display for displaying the output information of said date processor.
Claim 57
Independent Claim
127 words
An apparatus for detecting a particular particulate substance comprising: a light source; wavelength converter means for converting a wavelength of a ray of light provided from said light source to a wavelength selected to be substantially equal to the size of said particular particulate substance; a cell into which said particulate substance as the object of analysis is charged and which receives the ray of light whose wavelength is converted, and outputs a photoacoustic signal; a data processor for inputting said photoacoustic signal and determining the concentration of said particulate substance by use of a relationship between the magnitude of photoacoustic signals and the concentration of a particular particulate substance that is previously stored therein; and a display for displaying the output information of said date processor.
Claim 58
Dependent Claim
31 words
An apparatus according to claim 49, wherein said particulate substance is an immunoreaction product between an antigen and an antibody or between the antigen and a material supporting the antibody thereon.
Claim 59
Dependent Claim
39 words
An apparatus according to claim 50, wherein the ray of light to be radiated to said particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of said particulate substance.
Claim 60
Dependent Claim
33 words
An apparatus according to claim 50, wherein said support material for said antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 61
Dependent Claim
31 words
An apparatus according to claim 49, wherein said particulate substance is an immunoreaction product between an antibody and an antigen or between the antibody and a material supporting the antigen thereon.
Claim 62
Dependent Claim
39 words
An apparatus according to claim 53, wherein the ray of light to be radiated to said particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of said particulate substance.
Claim 63
Dependent Claim
33 words
An apparatus according to claim 53, wherein said support material for said antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 64
Dependent Claim
39 words
An apparatus according to claim 49, wherein the ray of light to be radiated to said particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of said particulate substance.
Claim 65
Independent Claim
111 words
An apparatus for detecting a particular particulate substance comprising: means for radiating a ray of light of a wavelength selected to be substantially equal to the size of said particular particulate substance to said particulate substance; a cell into which said particulate substance as the object of analysis is charged and which receives the ray of light from said radiating means, and outputs a photoacoustic signal; a reactor for reacting a sample and a reagent; means for moving said sample including an immunoreaction product from said reactor to said cell; and a data processor for inputting said photoacoustic signal and discriminating and outputting a photoacoustic signal from said particular particulate substance.
Claim 66
Independent Claim
111 words
An apparatus for detecting a particular particulate substance comprising: means for radiating a ray of light of a wavelength selected to be substantially equal to the size of said particular particulate substance to said particulate substance; a cell into which said particulate substance as the object of analysis is charged and which receives the ray of light from said radiating means, and outputs a photoacoustic signal; a reactor for reacting a sample and a reagent; means for moving said sample including an immunoreaction product from said reactor to said cell; and a data processor for inputting said photoacoustic signal and discriminating and outputting a photoacoustic signal from said particular particulate substance.
Claim 67
Dependent Claim
31 words
An apparatus according to claim 57, wherein said particulate substance is an immunoreaction product between an antigen and an antibody or between the antigen and a material support the antibody thereon.
Claim 68
Dependent Claim
39 words
An apparatus according to claim 58, wherein the ray of light to be radiated to said particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of said particulate substance.
Claim 69
Dependent Claim
33 words
An apparatus according to claim 58, wherein said support material for said antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 70
Dependent Claim
31 words
An apparatus according to claim 57, wherein said particulate substance is an immunoreaction product between an antibody and an antigen or between the antibody and a material support the antigen thereon.
Claim 71
Dependent Claim
39 words
An apparatus according to claim 61, wherein the ray of light to be radiated to said particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of said particulate substance.
Claim 72
Dependent Claim
33 words
An apparatus according to claim 61, wherein said support material for said antibody is a material having a small light absorption coefficient for the wavelength of the ray of light to be radiated.
Claim 73
Dependent Claim
39 words
An apparatus according to claim 57, wherein the ray of light to be radiated to said particulate substance is a ray of continuous light within a wavelength range capable of embracing the particle size distribution of said particulate substance.
References Cited (Backward Citations)
US4747685A
Cited
JPH102541A
Cited
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