Patent US4889919A successfully refreshed from Google Patents.
US4889919A
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Biologically active PDGF derived A-chain homodimers
Assignee: Panasonic Holdings Corp
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Filing: 1986-12-15
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Granted: 1989-12-26
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Expires: 2006-12-15
Abstract
Abstract Dimeric proteins having substantially the same biological activity as PDGF are disclosed. More specifically, the protein may have two substantially identical polypeptide chains, each of the chains being substantially homologous to the A-chain of PDGF. Alternatively, the protein may have two polypeptide chains that are substantially identical to the A-chain of PDGF. In addition, proteins comprising polypeptides that are variants or derivatives of the A-chain of PDGF are also disclosed. Therapeutic compositions containing these proteins and methods for enhancing the wound-healing process in warm-blooded animals are also disclosed.
Inventors
- 👤 Mark J. Murray
- 👤 James D. Kelly
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 12:56
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C07K14/435; C07K14/475; C07K14/49; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/62; C12N15/625; C12N15/63; C12N15/79; C12N15/80; C12N15/81; A; A61; A61K; A61K38/00; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/036; C07K2319/70; C07K2319/74; C07K2319/75; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/11011; C12N2740/11022; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/12; Y10S930/22; Y10S930/221
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
128 words
A substantially pure protein having two disulfide-bonded polypeptide chains, each of said chains comprising the amino acid sequence: V X1 K T R T V I Y E I P R S Q V D P T S A N F L I W F P X2 V E V K R X3 T G X4 X5 X6 T S S V K C Q P S R V H H R S V K V A K V E Y V R K K P K L K E V Q V R L E E H L E C A C A T T S L N P D Y R E wherein each of X1, X2, X3, X4, X5, and X6 is any amino acid.
Claim 2
Dependent Claim
128 words
A substantially pure protein having two disulfide-bonded polypeptide chains, each of said chains comprising the amino acid sequence: V X1 K T R T V I Y E I P R S Q V D P T S A N F L I W F P X2 V E V K R X3 T G X4 X5 X6 T S S V K C Q P S R V H H R S V K V A K V E Y V R K K P K L K E V Q V R L E E H L E C A C A T T S L N P D Y R E wherein each of X1, X2, X3, X4, X5, and X6 is any amino acid.
Claim 3
Dependent Claim
112 words
A substantially pure protein having two disulfide-bonded polypeptide chains, each of said chains comprising the amino acid sequence: Q V D P T S A N F L I W P P X2 V E V K R X3 T G X4 X5 X6 T S S V K C Q P S R V H H R S V K V A K V E Y V R K K P K L K E V Q V R L E E H L E C A C A T T S L N P D Y E wherein each of X2, X3, X4, X5, and X6 is any amino acid.
Claim 4
Dependent Claim
112 words
A substantially pure protein having two disulfide-bonded polypeptide chains, each of said chains comprising the amino acid sequence: Q V D P T S A N F L I W P P X2 V E V K R X3 T G X4 X5 X6 T S S V K C Q P S R V H H R S V K V A K V E Y V R K K P K L K E V Q V R L E E H L E C A C A T T S L N P D Y E wherein each of X2, X3, X4, X5, and X6 is any amino acid.
Claim 5
Dependent Claim
119 words
A substantially pure protein having two disulfide-bonded polypeptide chains, each of said chains comprising the amino acid sequence: V X1 K T R T V I Y E I P R S Q V D P T S A N F L I W P P X2 V E V K R X3 T G X4 X5 X6 T S S V K C Q P S R V H H R S V K V A K V E Y V R K K P K L K E V Q V R L E E H L E C A C A T wherein each of X1, X2, X3, X4, X5, and X6 is any amino acid.
Claim 6
Dependent Claim
119 words
A substantially pure protein having two disulfide-bonded polypeptide chains, each of said chains comprising the amino acid sequence: V X1 K T R T V I Y E I P R S Q V D P T S A N F L I W P P X2 V E V K R X3 T G X4 X5 X6 T S S V K C Q P S R V H H R S V K V A K V E Y V R K K P K L K E V Q V R L E E H L E C A C A T wherein each of X1, X2, X3, X4, X5, and X6 is any amino acid.
Claim 7
Dependent Claim
103 words
A substantially pure protein having two disulfide-bonded polypeptide chains, each of said chains comprising the amino sequence: Q V D P T S A N F L I W P P X2 V E V K R X3 T G X4 X5 X6 T S S V K C Q P S R V H H R S V K V A K V E Y V R K K P K L K E V Q V R L E E H L E C A C A T wherein each of X2, X3, X4, X5 and X6 is any amino acid.
Claim 8
Dependent Claim
103 words
A substantially pure protein having two disulfide-bonded polypeptide chains, each of said chains comprising the amino sequence: Q V D P T S A N F L I W P P X2 V E V K R X3 T G X4 X5 X6 T S S V K C Q P S R V H H R S V K V A K V E Y V R K K P K L K E V Q V R L E E H L E C A C A T wherein each of X2, X3, X4, X5 and X6 is any amino acid.
Claim 9
Dependent Claim
127 words
A substantially pure protein having two disulfide-bonded polypeptide chains, each of said chains comprising the amino acid sequence. S I E E A V P A V X1 K T R T V I Y E I P R S Q V D P T S A N F L I W P P X2 V E V K R X3 T G X4 X5 X6 T S S V K C Q P S R V H H R S V K V A K V E Y V R K K P K L K E V Q V R L E E H L E C A C A T wherein each of X1, X2, X3, X4, X5 and X6 is any amino acid.
Claim 10
Dependent Claim
127 words
A substantially pure protein having two disulfide-bonded polypeptide chains, each of said chains comprising the amino acid sequence. S I E E A V P A V X1 K T R T V I Y E I P R S Q V D P T S A N F L I W P P X2 V E V K R X3 T G X4 X5 X6 T S S V K C Q P S R V H H R S V K V A K V E Y V R K K P K L K E V Q V R L E E H L E C A C A T wherein each of X1, X2, X3, X4, X5 and X6 is any amino acid.
Claim 11
Dependent Claim
132 words
A substantially pure protein having two disulfide-bonded polypeptide chains, each of said chains comprising the amino acid sequence: S I E E A V P A V X1 K T R T V I Y E I P R S Q V D P T S A N F L I W P P X V E V K R X3 T G X4 X5 X6 T S S V K C Q P S V H H S V V A K V E Y V R K K P K L K E Q V R L E E H L E C A C A T T S L N P D Y R E wherein each of X1, X2, X3, X4, X5 and X6 is any amino acid.
Claim 12
Dependent Claim
132 words
A substantially pure protein having two disulfide-bonded polypeptide chains, each of said chains comprising the amino acid sequence: S I E E A V P A V X1 K T R T V I Y E I P R S Q V D P T S A N F L I W P P X V E V K R X3 T G X4 X5 X6 T S S V K C Q P S V H H S V V A K V E Y V R K K P K L K E Q V R L E E H L E C A C A T T S L N P D Y R E wherein each of X1, X2, X3, X4, X5 and X6 is any amino acid.
Claim 13
Dependent Claim
16 words
A protein according to any one of claims 1 to 48 wherein said protein is unglycosylated.
Claim 14
Dependent Claim
17 words
A protein according to any one of claims 1 to 48 wherein X6 is a glutamine residue.
Claim 15
Dependent Claim
18 words
A protein according to any one of claims 3, 5 and 6 wherein X1 is a serine residue.
Claim 16
Dependent Claim
18 words
A composition comprising a protein according to any of claims 1-9 and a physiologically acceptable carrier or diluent.
Claim 17
Dependent Claim
22 words
The composition of claim 10 wherein said carrier or diluent is selected from the group consisting of albumin, sterile water and saline.
Claim 18
Dependent Claim
9 words
The composition of claim 10, further comprising an adjuvant.
Claim 19
Dependent Claim
24 words
The composition of claim 12 wherein said adjuvant is selected from the group consisting of collagen, hyaluronic acid, fibronection, factor XIII, and an antibiotic.
Claim 20
Dependent Claim
25 words
The composition of claim 10 wherein said protein is present in a concentration of from about 10 ng/ml to about 100 ug/ml of total volume.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-12-25 | 1993-06-26 | 1993-12-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-12-25 | 1997-06-26 | 1997-12-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-12-25 | 2001-06-26 | 2001-12-26 | $7700.00 | Window Closed / Lapsed |