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US4884200A
Expired / Lapsed
US
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Signal detecting method in autoradiography
Assignee: Fujifilm Holdings Corp
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Filing: 1984-03-30
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Granted: 1989-11-28
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Expires: 2004-03-30
Abstract
Abstract A signal detecting method in autoradiography, which is applied to an autoradiograph having locational information on radioactively labeled substances such as radioactively labeled biopolymers distributed one-dimensionally, being recorded on a radiosensitive material as a visible image, which comprises steps of: (1) preliminarily scanning a part of the radiosensitive material with a light and photoelectrically reading out a portion of the visualized autoradiograph to give an electric signal, to which a signal processing is applied, to determine one-dimensional distribution direction of the radioactively labeled substances; and (2) finally scanning the radiosensitive material with a light along said one-dimensional distribution direction and photoelectrically reading out the visualized autoradiograph, to obtain the locational information on the radioactively labeled substances as a digital signal.
Inventors
- 👤 Tsutomu Kimura
- 👤 Kazuhiro Hishinuma
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 14:59
CPC Classifications
G; G01; G01T; G01T1/00; G01T1/16; G01T1/20; G01T1/2012; G01T1/2014; G01T1/29; G01T1/2914; G01T1/2921; G01T1/2942
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
198 words
A signal detecting method in autoradiography, which is applied to an autoradiograph having locational information on radioactively labeled substances distributed one-dimensionally, being recorded on a radiosensitive material as a visible image, which comprises steps of: (1) scanning the radiosensitive material with a light in at least two different positions in such a manner that each scanning traverses the one-dimensional distribution of the radioactively labeled substances and photoelectrically reading out a portion of the visualized autoradiograph to give an electric signal, to which is applied a signal processing comprising stages of: detecting distribution points of the radioactively labeled substances for each scanning, and preparing a continuous line selected from the group consisting of a straight line, a polygonal line and a curved line along the corresponding distribution points of the radioactively labeled substances for each scanning, to assign said continuous line to one-dimensional distribution direction of the radioactively labeled substances; and (2) scanning the radiosensitive material with a light along said one-dimensional distribution direction of the radioactively labeled substances determined in the step (1) and photoelectrically reading out the visualized autoradiograph, to obtain the locational information on the radioactively labeled substances indicated by said autoradiograph as a digital signal.
Claim 2
Dependent Claim
198 words
A signal detecting method in autoradiography, which is applied to an autoradiograph having locational information on radioactively labeled substances distributed one-dimensionally, being recorded on a radiosensitive material as a visible image, which comprises steps of: (1) scanning the radiosensitive material with a light in at least two different positions in such a manner that each scanning traverses the one-dimensional distribution of the radioactively labeled substances and photoelectrically reading out a portion of the visualized autoradiograph to give an electric signal, to which is applied a signal processing comprising stages of: detecting distribution points of the radioactively labeled substances for each scanning, and preparing a continuous line selected from the group consisting of a straight line, a polygonal line and a curved line along the corresponding distribution points of the radioactively labeled substances for each scanning, to assign said continuous line to one-dimensional distribution direction of the radioactively labeled substances; and (2) scanning the radiosensitive material with a light along said one-dimensional distribution direction of the radioactively labeled substances determined in the step (1) and photoelectrically reading out the visualized autoradiograph, to obtain the locational information on the radioactively labeled substances indicated by said autoradiograph as a digital signal.
Claim 3
Dependent Claim
56 words
The signal detecting method in autoradiography as claimed in claim 1, wherein said scanning with the light in step (1) is carried out on the radiosensitive material in at least two different positions in such a manner that the scannings traversing the one-dimensional distribution of the radioactively labeled substances are made in parallel with each other.
Claim 4
Dependent Claim
76 words
The signal detecting method in autoradiography as claimed in claim 1, wherein said step (1) comprises: scanning the radiosensitive material with a light in two different positions to obtain an electric signal, to which is applied a signal processing comprising stages of: detecting distribution points of the radioactively labeled substances in two positions, and preparing a straight line between these two distribution points, to assign said line to one-dimensional distribution direction of the radioactively labeled substances.
Claim 5
Dependent Claim
54 words
The signal detecting method in autoradiography as claimed in claim 1, wherein the position and width of said scanning on the radiosensitive material with the light in the step (1) are set prior to the signal detecting as to match with the conditions of the one-dimensional distribution of the radioactively labeled substances under analysis.
Claim 6
Dependent Claim
36 words
The signal detecting method in autoradiography as claimed in claim 1, wherein the position and width of said scanning on the radiosensitive material with the light in the step (2) are determined in the step (1).
Claim 7
Dependent Claim
46 words
The signal detecting method in autoradiography as claimed in any one of the claims 1 through 5, wherein said one-dimensionally distributed radioactively labeled substances are biopolymers, derivatives thereof, or cleavage products thereof, labeled with a radioactive element and resolved in one-dimensional direction on the support medium.
Claim 8
Dependent Claim
23 words
The signal detecting method in autoradiography as claimed in claim 6, wherein said biopolymers are nucleic acids, derivatives thereof, or cleavage products thereof.
References Cited (Backward Citations)
US31847A
Cited
USRE31847E
Cited
SU738602A1
Cited
US4258264A
Cited
US4315318A
Cited
US4320415A
Cited
US4526865A
Cited
US4496973A
Cited
US4389670A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-11-27 | 1993-05-29 | 1993-11-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-11-27 | 1997-05-29 | 1997-11-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-11-27 | 2001-05-29 | 2001-11-28 | $7700.00 | Window Closed / Lapsed |