Method of performing medical analysis on a liquid sample using at least one dry reagent, and apparatus for the method

Claim 1

A method of performing medical analysis on a liquid sample using at least one dry reagent together with a diluent, using a container having internal compartments including: a storage chamber for said liquid sample connected via a capillary duct to one end of a calibrated cell whose other end is connected via a capillary duct to an overflow chamber; a storage chamber for said liquid diluent; a pouring chamber, a capillary duct connected at one end to said pouring chamber and on the other end to said calibrated cell, a capillary duct connected at one end to said pouring chamber and at the outer end to said diluent storage chamber, and a further capillary duct connected at one end to said pouring chamber and at the other end with a first reaction chamber suitable for containing a dry reagent; and two reaction chambers suitable for containing dry reagents situated on either side of said first reaction chamber, and connected via capillary ducts having different angular orientations; a lid for closing said container fitted both with a sample storing receptacle connected directly with said sample storage chamber and situated thereabove, and with a removable stopper which penetrates into said pouring chamber to close the ends of said capillary ducts leading thereto; and means provided for mounting said container on the turntable of a centrifuge with the axis of the container offset from the axis of the turntable for rotation of the container about said container axis to a plurality of different predetermined positions relative to the turntable and through a given angle to permit selective alignment of a given capillary duct with the direction of centrifuging force resulting from turntable rotation; said method comprising the steps of: providing a liquid diluent initially in said diluent storage chamber and dry reagents in respective ones of said reaction chambers; inserting said sample into the sample storing receptacle and then flowing said sample under gravity into said sample storage chamber, removing said stopper, mounting said container on said centrifuge turntable, operating said turntable and performing a plurality of successive centrifuging operations, while setting the angular position of the container on each occasion from among said predetermined angular positions as a function of the orientation of the capillary duct concerned relative to the direction of centrifugal force in such a manner as to cause said sample to pass successively via said capillary ducts from said sample storage chamber into said calibrated cell, then into said pouring chamber, and then into said first reaction chamber, and subsequently into each of the other reaction chambers.

Claim 2

A method of performing medical analysis on a liquid sample using at least one dry reagent together with a diluent, using a container having internal compartments including: a storage chamber for said liquid sample connected via a capillary duct to one end of a calibrated cell whose other end is connected via a capillary duct to an overflow chamber; a storage chamber for said liquid diluent; a pouring chamber, a capillary duct connected at one end to said pouring chamber and on the other end to said calibrated cell, a capillary duct connected at one end to said pouring chamber and at the outer end to said diluent storage chamber, and a further capillary duct connected at one end to said pouring chamber and at the other end with a first reaction chamber suitable for containing a dry reagent; and two reaction chambers suitable for containing dry reagents situated on either side of said first reaction chamber, and connected via capillary ducts having different angular orientations; a lid for closing said container fitted both with a sample storing receptacle connected directly with said sample storage chamber and situated thereabove, and with a removable stopper which penetrates into said pouring chamber to close the ends of said capillary ducts leading thereto; and means provided for mounting said container on the turntable of a centrifuge with the axis of the container offset from the axis of the turntable for rotation of the container about said container axis to a plurality of different predetermined positions relative to the turntable and through a given angle to permit selective alignment of a given capillary duct with the direction of centrifuging force resulting from turntable rotation; said method comprising the steps of: providing a liquid diluent initially in said diluent storage chamber and dry reagents in respective ones of said reaction chambers; inserting said sample into the sample storing receptacle and then flowing said sample under gravity into said sample storage chamber, removing said stopper, mounting said container on said centrifuge turntable, operating said turntable and performing a plurality of successive centrifuging operations, while setting the angular position of the container on each occasion from among said predetermined angular positions as a function of the orientation of the capillary duct concerned relative to the direction of centrifugal force in such a manner as to cause said sample to pass successively via said capillary ducts from said sample storage chamber into said calibrated cell, then into said pouring chamber, and then into said first reaction chamber, and subsequently into each of the other reaction chambers.