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US4876190A
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Peridinin-chlorophyll complex as fluorescent label
Assignee: Becton Dickinson and Co
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Filing: 1987-10-21
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Granted: 1989-10-24
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Expires: 2007-10-21
Abstract
Abstract Peridinin-chlorophyll-protein complexes are provided for use as fluorescent labels and are particularly useful in diagnostic assays employing as a reagent a fluorescent compound conjugated to a member of a specific binding pair, wherein the pair consists of a biochemical ligand and a receptor and the diagnostic assay comprises a step in which the conjugate binds to its complementary binding-pair member.
Inventors
- 👤 Diether J. Recktenwald
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 15:26
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/58; G01N33/582; Y; Y10; Y10S; Y10S435/00; Y10S435/968; Y10S436/00; Y10S436/80; Y10S530/00; Y10S530/802
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
70 words
In a diagnostic assay employing as a reagent a fluorescent compound conjugated to a member of a specific binding pair, wherein said pair comprises two biochemical molecules that bind specifically to each other and said diagnostic assay comprises a step in which the conjugated member of the specific binding pair binds to the other member of the specific binding pair, the improvement wherein said flurescent compound is a peridinin-chloro-phyll-protein complex.
Claim 2
Dependent Claim
70 words
In a diagnostic assay employing as a reagent a fluorescent compound conjugated to a member of a specific binding pair, wherein said pair comprises two biochemical molecules that bind specifically to each other and said diagnostic assay comprises a step in which the conjugated member of the specific binding pair binds to the other member of the specific binding pair, the improvement wherein said flurescent compound is a peridinin-chloro-phyll-protein complex.
Claim 3
Dependent Claim
21 words
The diagnostic assay of claim 1, wherein the other member of said binding pair is a cell or cell surface antigen.
Claim 4
Dependent Claim
14 words
The diagnostic assay of claim 1, wherein said complex is a peridinin-chlorophyll a-protein complex.
Claim 5
Dependent Claim
26 words
The diagnostic assay of claim 1, wherein said complex is a peridinin-chlorophyll a-protein complex and said conjugated member of the specific binding pair is an immunoglobulin.
Claim 6
Dependent Claim
29 words
A fluorescent compound comprising a peridinin-chlorophyll-protein complex conjugated to a member of a specific binding pair, wherein said pair comprises two biochemical molecules that bind specifically to each other.
Claim 7
Dependent Claim
29 words
A fluorescent compound comprising a peridinin-chlorophyll-protein complex conjugated to a member of a specific binding pair, wherein said pair comprises two biochemical molecules that bind specifically to each other.
Claim 8
Dependent Claim
17 words
The compound of claim 5, wherein said conjugated member of said specific binding pair is a protein.
Claim 9
Dependent Claim
26 words
The compound of claim 6, wherein said conjugated member of said specific binding pair is such that it binds to a cell surface receptor or antigen.
Claim 10
Dependent Claim
34 words
The compound of claim 7, wherein said conjugated memer of said specific binding pair is an antibody and said specific binding pair is said antibody and an antigen to which said antibody specifically binds.
Claim 11
Dependent Claim
13 words
The compound of claim 5, wherein said complex is a peridinin-chlorophyll a-protein complex.
Claim 12
Dependent Claim
32 words
The compound of claim 5, wherein said conjugated member of said specific binding pair is an antigen and said specific binding pair is said antigen and an antibody specific for said antigen.
References Cited (Backward Citations)
US4745285A
Cited
US4745285B1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-10-23 | 1993-04-24 | 1993-10-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-10-23 | 1997-04-24 | 1997-10-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-10-23 | 2001-04-24 | 2001-10-24 | $7700.00 | Window Closed / Lapsed |