US4870024A
Expired / Lapsed
US
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Concurrent analysis of a fluid sample for calcium and a monovalent ion
Assignee: IL Holding Spa
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Filing: 1987-07-17
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Granted: 1989-09-26
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Expires: 2007-07-17
Abstract
Abstract A method for determining the concentrations of total calcium and at least one monovalent ion in a sample includes the steps of mixing the sample with a diluent that has a pH within the range pH 6.5 to 7.0 and includes 2-amino-2-hydroxymethyl-1,3 propanediol phosphate and is free of the monovalent ion. An aliquot of the diluted sample is concurrently contacted with a calcium-specific ion selective electrode and an ion selective electrode specifically responsive to the monovalent ion, the response of the calcium-specific ion selective electrode is measured as an indication of the concentration of total calcium in the sample, and the response of the monovalent ion specific ion selective electrode is measured as an indication of the concentration of the monovalent ion in the sample.
Inventors
- 👤 John Musacchio
- 👤 Carolyn Bergkuist
- 👤 Angelo Manzoni
- 👤 Pietro Premoli
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 05:08
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/84
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
169 words
A method for determining the concentrations of total calcium and least one monovalent ion in a sample which may contain protein bound calcium, comprising the steps of admixing said sample with a diluent, said diluent comprising 2 amino 2 hydroxymethyl-1,3-propanediol phosphate, said diluent having a pH within the range pH 6.5 to pH 7.0, said diluent being free of said monovalent ion, said admixing forming a diluted sample in which any said protein bound calcium in said sample is rendered detectable by a calcium specific ion selective electrode that is brought into contact with said diluted sample, contacting an aliquot of said diluted sample with a calcium specific ion selective electrode and an ion selective electrode specifically responsive to said monovalent ion, measuring the response of said calcium specific ion selective electrode as an indication of the concentration of total calcium in said sample, and measuring the response of said monovalent ion specific ion selective electrode as an indication of the concentration of said monovalent ion in said sample.
Claim 2
Independent Claim
169 words
A method for determining the concentrations of total calcium and least one monovalent ion in a sample which may contain protein bound calcium, comprising the steps of admixing said sample with a diluent, said diluent comprising 2 amino 2 hydroxymethyl-1,3-propanediol phosphate, said diluent having a pH within the range pH 6.5 to pH 7.0, said diluent being free of said monovalent ion, said admixing forming a diluted sample in which any said protein bound calcium in said sample is rendered detectable by a calcium specific ion selective electrode that is brought into contact with said diluted sample, contacting an aliquot of said diluted sample with a calcium specific ion selective electrode and an ion selective electrode specifically responsive to said monovalent ion, measuring the response of said calcium specific ion selective electrode as an indication of the concentration of total calcium in said sample, and measuring the response of said monovalent ion specific ion selective electrode as an indication of the concentration of said monovalent ion in said sample.
Claim 3
Dependent Claim
14 words
The method of claim 1 wherein said monovalent ion is sodium, potassium, or chloride.
Claim 4
Dependent Claim
13 words
The method of claim 1 wherein said diluent further comprises a reducing agent.
Claim 5
Dependent Claim
12 words
The method of claim 3 wherein said reducing agent is an aldehyde.
Claim 6
Dependent Claim
12 words
The method of claim 1 wherein said diluent further comprises a surfactant.
Claim 7
Dependent Claim
13 words
The method of claim 5 wherein said surfactant is a non ionic surfactant.
Claim 8
Dependent Claim
56 words
The method of claim 1 and further including the step of contacting said calcium specific ion selective electrode and said ion selective electrode specifically responsive to said monovalent ion with a reference solution that has the same chemical composition as said diluent except that said reference solution also contains calcium ion and said monovalent ion sources.
Claim 9
Dependent Claim
22 words
The method of claim 1 wherein the volume to volume ratio of said sample to said diluent is between 1:30 and 1:60.
Claim 10
Dependent Claim
22 words
The method of claim 1 wherein the concentration of said 2-amino-2-hydroxymethyl-1,3-propanediol phosphate in said diluent is at least 0.1 millimol per liter.
Claim 11
Dependent Claim
23 words
The method of claim 1 wherein the concentration of said 2-amino-2-hydroxymethyl-1,3-propanediol phosphate in said diluent is between 0.1 and 0.2 millimol per liter.
Claim 12
Dependent Claim
38 words
The method of claim 1 wherein chloride-specific, sodium specific and potassium specific ion selective electrodes are contacted with said aliquot of diluted sample concurrently with the contacting of aliquot of diluted sample with said calcium-specific ion selective electrode.
Claim 13
Independent Claim
249 words
A method for determining the concentration of calcium, chloride, sodium, and potassium in a sample of a biological fluid which may contain protein-bound calcium, comprising the steps of (a) admixing said sample with a diluent, said diluent comprising 2-amino-2-hydroxymethyl-1,3-propanediol phosphate, said diluent having a pH within the range pH 6.5 to pH 7.0, said diluent being free of ions of calcium, sodium, potassium, and chloride, said admixing forming a diluted sample in which any said protein bound calcium in said sample is rendered detectable by a calcium-specific ion selective electrode that is brought into contact with said diluted sample, (b) contacting an aliquot of said diluted sample with a calcium-specific ion selective electrode, said calcium specific ion selective electrode comprising a calcium ionophore capable of selectively binding calcium ions, (c) measuring the response of said calcium specific ion selective electrode as an indication of the concentration of total calcium in said sample, (d) contacting said aliquot with a chloride specific ion selective electrode, (e) measuring the response of said chloride specific ion selective electrode as an indication of the concentration of chloride in said sample, (f) contacting said aliquot with a sodium specific ion selective electrode, (g) measuring the response of said sodium-specific ion selective electrode as an indication of the concentration of sodium in said sample, (h) contacting said aliquot with a potassium-specific ion selective electrode, and (i) measuring the response of said potassium-specific ion selective electrode as an indication of the concentration of potassium in said sample.
Claim 14
Independent Claim
249 words
A method for determining the concentration of calcium, chloride, sodium, and potassium in a sample of a biological fluid which may contain protein-bound calcium, comprising the steps of (a) admixing said sample with a diluent, said diluent comprising 2-amino-2-hydroxymethyl-1,3-propanediol phosphate, said diluent having a pH within the range pH 6.5 to pH 7.0, said diluent being free of ions of calcium, sodium, potassium, and chloride, said admixing forming a diluted sample in which any said protein bound calcium in said sample is rendered detectable by a calcium-specific ion selective electrode that is brought into contact with said diluted sample, (b) contacting an aliquot of said diluted sample with a calcium-specific ion selective electrode, said calcium specific ion selective electrode comprising a calcium ionophore capable of selectively binding calcium ions, (c) measuring the response of said calcium specific ion selective electrode as an indication of the concentration of total calcium in said sample, (d) contacting said aliquot with a chloride specific ion selective electrode, (e) measuring the response of said chloride specific ion selective electrode as an indication of the concentration of chloride in said sample, (f) contacting said aliquot with a sodium specific ion selective electrode, (g) measuring the response of said sodium-specific ion selective electrode as an indication of the concentration of sodium in said sample, (h) contacting said aliquot with a potassium-specific ion selective electrode, and (i) measuring the response of said potassium-specific ion selective electrode as an indication of the concentration of potassium in said sample.
Claim 15
Dependent Claim
17 words
The method of claim 12 wherein said contacting steps (b), (d), (f) and (h) are performed concurrently.
Claim 16
Dependent Claim
16 words
The method of claim 12 wherein said diluent further comprises a reducing agent and a surfactant.
Claim 17
Dependent Claim
20 words
The method of claim 14 wherein said reducing agent is an aldehyde and said surfactant is a non ionic surfactant.
Claim 18
Dependent Claim
65 words
The method of claim 12 further comprising the steps of (j) contacting said ion selective electrodes with an aliquot of a reference solution, said reference solution comprising 2-amino-2-hydroxymethyl-1,3-propanediol phosphate and known concentrations of the ions of each of calcium, sodium, chloride, and potassium, and (k) measuring the response of said selective electrodes to said known concentrations of said ions as a calibration of said electrodes.
Claim 19
Dependent Claim
22 words
The method of claim 16 wherein each of said diluent and said reference solution further comprises a reducing agent and a surfactant.
Claim 20
Dependent Claim
19 words
The method of claim 17 wherein said reducing agent is an aldehyde and said surfactant is a non-ionic surfactant.
Claim 21
Dependent Claim
22 words
The method of claim 18 wherein the volume to volume ratio of said sample to said diluent is between 1:30 and 1:60.
Claim 22
Dependent Claim
22 words
The method of claim 19 wherein the concentration of said 2-amino-2-hydroxymethyl-1,3-propanediol phosphate in said diluent is at least 0.1 millimol per liter.
Claim 23
Dependent Claim
23 words
The method of claim 20 wherein the concentration of said 2-amino-2-hydroxymethyl-1,3-propanediol phosphate in said diluent is between 0.1 and 0.2 millimol per liter.
References Cited (Backward Citations)
US4618587A
Cited
EP0166944A1
Cited
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