EP0166944A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method of releasing protein-bound calcium
Assignee: Nova Biomedical Corp
•
Filing: 1985-05-23
•
Granted: —
•
Expires: 2005-05-23
Abstract
Abstract releasing reagent for releasing, as ionized calcium, protein-bound calcium in a biological sample for measurement of the ionized calcium, together with any ionized calcium originally present in the sample, using a calcium ion specific electrode, the releasing reagent including monochloroacetate salt, formate salt, and formic acid, in concentrations sufficient to render the pH of the sample, when mixed with the reagent, in the range of 4.0 to 5.5.
Inventors
- 👤 Chung Chang Young
- 👤 Linda M. Mulholland
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 18:16
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/84
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
71 words
A releasing reagent for releasing, as ionized calcium, protein-bound calcium in a liquid sample for measurement of said ionized calcium, together with any ionized calcium originally present in said sample, using a calcium ion specific electrode, said releasing reagent characterized in that it comprises formate salt and formic acid, in concentrations sufficient to render the pH of said sample, when mixed with said reagent, in the range of 4.0 to 5.5.
Claim 2
Dependent Claim
71 words
A releasing reagent for releasing, as ionized calcium, protein-bound calcium in a liquid sample for measurement of said ionized calcium, together with any ionized calcium originally present in said sample, using a calcium ion specific electrode, said releasing reagent characterized in that it comprises formate salt and formic acid, in concentrations sufficient to render the pH of said sample, when mixed with said reagent, in the range of 4.0 to 5.5.
Claim 3
Dependent Claim
35 words
The releasing reagent of claim 1 further characterized in that the concentration of formic acid is between 0.105 Molar and 0.195 Molar and the concentration of formate salt is between 0.21 Molar and 0.39 Molar.
Claim 4
Dependent Claim
35 words
The releasing reagent of claim 2 further characterized in that said formic acid is present in a concentration of about 0.15 Molar, and said formate salt is present in a concentration of about 0.30 Molar.
Claim 5
Dependent Claim
37 words
The releasing reagent of any of the preceding claims wherein said sample contains protein and said releasing reagent is further characterized in that it comprises a component capable of inhibiting precipation of said protein in said sample.
Claim 6
Dependent Claim
22 words
The releasing reagent of claim 4 further characterized in that said precipitation inhibiting component comprises a salt of a strong organic acid.
Claim 7
Dependent Claim
18 words
The releasing reagent of claim 5 further characterized in that said strong organic acid comprises an acetate salt.
Claim 8
Dependent Claim
16 words
The releasing reagent of claim 6 further characterized in that said acetate salt comprises monochloroacetate salt.
Claim 9
Dependent Claim
24 words
The releasing reagent of claim 7 further characterized in that said monochloroacetate salt is present in a concentration between 0.385 Molar and 0.715 Molar.
Claim 10
Dependent Claim
23 words
The releasing reagent of claim 8 further characterized in that said releasing reagent further comprises monochloroacetate as a concentration of about 0.55 Molar.
Claim 11
Dependent Claim
16 words
The releasing reagent of claim 6 further characterized in that said acetate salt comprises trifluoroacetate salt.
Claim 12
Dependent Claim
24 words
The releasing reagent of claim 10 further characterized in that said trifluoroacetate salt is present in a concentration between 0.224 Molar and 0.416 Molar.
Claim 13
Dependent Claim
22 words
The releasing reagent of claim 11 further characterized in that said releasing further comprises trifluoroacetate at a concentration of about 0.32 Molar.
Claim 14
Dependent Claim
17 words
The releasing reagent of any of the preceding claims further characterized in that it comprises ammonium chloride.
Claim 15
Dependent Claim
25 words
The releasing reagent of claim 13 further characterized in that said ammonium chloride is present in a concentration of between 1.5 Molar and 3.0 Molar.
Claim 16
Dependent Claim
22 words
The releasing reagent of claim 14 further characterized in that said ammonium chloride is present in a concentration of about 2.0 Molar.
Claim 17
Dependent Claim
49 words
The releasing reagent of any of claims 13-15 wherein said reagent is for use in apparatus in which said reagent is added to said sample at the same time a reference solution is added to said sample, said releasing reagent further characterized in that it comprises said reference solution.
Claim 18
Independent Claim
75 words
The method of releasing protein-bound calcium as ionized calcium in a liquid sample for measurement of said ionized calcium, together with any ionized calcium originally present in said sample, using a calcium ion specific electrode, characterized in that said sample is mixed with a releasing reagent comprising formate salt and formic acid in concentrations sufficient to render the pH of the mixture of said sample and said reagent in the range of 4.0 to 5.5.
Claim 19
Independent Claim
75 words
The method of releasing protein-bound calcium as ionized calcium in a liquid sample for measurement of said ionized calcium, together with any ionized calcium originally present in said sample, using a calcium ion specific electrode, characterized in that said sample is mixed with a releasing reagent comprising formate salt and formic acid in concentrations sufficient to render the pH of the mixture of said sample and said reagent in the range of 4.0 to 5.5.
Claim 20
Dependent Claim
34 words
The method of claim 17 further characterized in that the concentration of formic acid is between 0.105 Molar and 0.195 Molar and the concentration of formate salt is between 0.21 Molar and 0.39 Molar.
Claim 21
Dependent Claim
34 words
The method of claim 18 further characterized in that said formic acid is present in a concentration of about 0.15 Molar, and said formate salt is present in a concentration of about 0.30 Molar.
Claim 22
Independent Claim
36 words
The method of any of the preceding claims wherein said sample contains protein and said releasing reagent is further characterized in that it comprises a component capable of inhibiting precipation of said protein in said sample.
Claim 23
Dependent Claim
21 words
The method of claim 20 further characterized in that said precipitation inhibiting component comprises a salt of a strong organic acid.
Claim 24
Dependent Claim
17 words
The method of claim 21 further characterized in that said strong organic acid comprises an acetate salt.
Claim 25
Dependent Claim
15 words
The method of claim 22 further characterized in that said acetate salt comprises monochloroacetate salt.
Claim 26
Dependent Claim
23 words
The method of claim 23 further characterized in that said monochloroacetate salt is present in a concentration between 0.385 Molar and 0.715 Molar.
Claim 27
Dependent Claim
22 words
The method of claim 24 further characterized in that said releasing reagent further comprises monochloroacetate as a concentration of about 0.55 Molar.
Claim 28
Dependent Claim
15 words
The method of claim 22 further characterized in that said acetate salt comprises trifluoroacetate salt.
Claim 29
Dependent Claim
23 words
The method of claim 26 further characterized in that said trifluoroacetate salt is present in a concentration between 0.224 Molar and 0.416 Molar.
Claim 30
Dependent Claim
21 words
The method of claim 27 further characterized in that said releasing further comprises trifluoroacetate at a concentration of about 0.32 Molar.
Claim 31
Independent Claim
16 words
The method of any of the preceding claims further characterized in that it comprises ammonium chloride.
Claim 32
Dependent Claim
24 words
The method of claim 29 further characterized in that said ammonium chloride is present in a concentration of between 1.5 Molar and 3.0 Molar.
Claim 33
Dependent Claim
21 words
The method of claim 30 further characterized in that said ammonium chloride is present in a concentration of about 2.0 Molar.
Claim 34
Independent Claim
48 words
The method of any of claims 29-31 wherein said reagent is for use in apparatus in which said reagent is added to said sample at the same time a reference solution is added to said sample, said releasing reagent further characterized in that it comprises said reference solution.
References Cited (Backward Citations)
US4382122A
Cited
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.