Automatic chromatography process

Abstract

Abstract Automatic chromatography apparatus comprises a column packed with adsorbent or attractant material; means for supplying an elutant solution to the column; loading means for loading controlled quantitites of a material into the column, the material containing a substance to be eluted; automatic control means which control the loading means so as to load the column with a first charge of the material and subsequently to load the column with a second charge of the material, the first charge being sufficiently small to ensure that the capacity of the column is not exceeded and the second charge being sufficiently large to ensure that the capacity of the column is exceeded; first monitoring means for monitoring and controlling flow rate to provide accurate flow information to enable peak area integration; second monitoring means which monitor the peaks of elution of the substance from the column resulting from the first and second charges of material; processing means connected to the monitoring means and arranged to integrate the elution peak with respect to the first charge of material so as to derive the concentration of the substance in the material, and to determine from the peak of elution of the substance from the column resulting from the second charge of material the maximum practical capacity of the column for the said substance.

Inventors

• 👤 Andrew C. Kenney
• 👤 Philip W. Thompson
• 👤 John L. Harris

Bibliographic Details