US4614620A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing i-brassicasterol
Assignee: Kureha Corp
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Filing: 1985-02-19
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Granted: 1986-09-30
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Expires: 2005-02-19
Abstract
Abstract Disclosed herein is a process for producing i-brassicasterol, comprising charging a mixture of i-sterols derived from a mixture of sterols including brassicasterol into a column packed with a filler obtained by chemically binding alkyl group(s) of 15 to 24 carbon atoms to silica, and subjecting said mixture of i-sterols to reversed-phase partition column-chromatography while using (i) an alcohol of one to three carbon atoms or a mixture therof, or (ii) a mixed solvent comprising more than 50% by volume of said alcohol(s) and a solvent other than said alcohol(s) as an eluent, thereby isolating and purifying i-brassicasterol.
Inventors
- 👤 Yutaka Konai
- 👤 Shoichiro Hayashi
- 👤 Yoshikazu Kubota
- 👤 Kouichi Kodama
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 16:47
CPC Classifications
C; C07; C07J; C07J53/00
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
159 words
A process for producing i-brassicasterol, comprising charging a mixture of i-sterols dervies from a mixture of sterols including brassicasterol into a column packed with a filler obtained by chemically binding alkyl group(s) of 15 to 24 carbon atoms to silica, and subjecting said mixture of i-sterols to reversed-phase partition column-chromatography while using (i) an alcohol of one to three carbon atoms or a mixture thereof, (ii) a mixed solvent comprising more than 50% by volume of said alcohol(s) and less than 50% by volume of water, or (iii) a mixed solvent comprising more than 50% by volume of said alcohol(s) and less than 50% by volume of a mixture of water and organic solvent(s) having a parameter of solvent strength (ε o ) of 0.4 to 0.7 selected from the group consisting of acetone, tetrahydrofuran, dioxane, chloroform, dichloromethane, methyl ethyl ketone, ethyl acetate, methyl acetate, dimethylsulfoxide, acetonitrile and a mixture thereof, as an eluent, thereby isolating and purifying i-brassicasterol.
Claim 2
Dependent Claim
159 words
A process for producing i-brassicasterol, comprising charging a mixture of i-sterols dervies from a mixture of sterols including brassicasterol into a column packed with a filler obtained by chemically binding alkyl group(s) of 15 to 24 carbon atoms to silica, and subjecting said mixture of i-sterols to reversed-phase partition column-chromatography while using (i) an alcohol of one to three carbon atoms or a mixture thereof, (ii) a mixed solvent comprising more than 50% by volume of said alcohol(s) and less than 50% by volume of water, or (iii) a mixed solvent comprising more than 50% by volume of said alcohol(s) and less than 50% by volume of a mixture of water and organic solvent(s) having a parameter of solvent strength (ε o ) of 0.4 to 0.7 selected from the group consisting of acetone, tetrahydrofuran, dioxane, chloroform, dichloromethane, methyl ethyl ketone, ethyl acetate, methyl acetate, dimethylsulfoxide, acetonitrile and a mixture thereof, as an eluent, thereby isolating and purifying i-brassicasterol.
Claim 3
Dependent Claim
41 words
A process according to claim 1, wherein said mixture of sterols including brassicaterol is an unsaponifiable material obtained from a distillate from natural oils and fats in the step of deodorization thereof or a distillation residue of distillation of fatty acids.
Claim 4
Dependent Claim
42 words
A process according to claim 1, wherein said mixture of i-sterols is obtained by converting said mixture of sterols including brassicaterol into an ester of sulfonic acid with alkylsulfonyl halide or arylsulfonyl halide, and hydrolyzing the thus obtained ester of sulfonic acid.
References Cited (Backward Citations)
JPS6013800A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-09-29 | 1990-03-31 | 1990-09-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-09-29 | 1994-03-31 | 1994-09-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-09-29 | 1998-03-31 | 1998-09-30 | $7700.00 | Window Closed / Lapsed |