US4859584A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell growth rate determination by measurement of changes in cyanine dye levels in plasma membranes
Assignee: SKB AND ZYNAXIS CELL SCIENCE Inc A CORP OF PENNSYLVANIA
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Filing: 1986-10-31
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Granted: 1989-08-22
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Expires: 2006-10-31
Abstract
Abstract Methods for determining growth rate of cells growing in vivo and in vitro. Cells are labelled with cyanine dyes and changes in plasma membrane cyanine dye levels are used to determine growth rate. Cell growth rate determinations are utilized to monitor transplanted bone marrow cell engraftment and post-surgical corneal epitheal cell growth. The invented methods also are used to determine tumor cell sensitivity to cancer therapeutic agents, yeast sensitivity to antifungal agents, and bacteria sensitivity to antibacterial agents.
Inventors
- 👤 Paul K. Horan
- 👤 Bruce D. Jensen
- 👤 Sue E. Slezak
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 01:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; A; A61; A61K; A61K49/00; A61K49/001; A61K49/0013; A61K49/0017; A61K49/0019; A61K49/0021; A61K49/0032; C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/18; G01N33/50; G01N33/5005
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
44 words
A method for determining cell growth rate that comprises measuring changes in levels of cyanine dye in the plasma membranes of daughter cells derived from parent cells labelled with a cyanine dye wherein fluorescence is used to measure changes in levels of cyanine dye.
Claim 2
Independent Claim
44 words
A method for determining cell growth rate that comprises measuring changes in levels of cyanine dye in the plasma membranes of daughter cells derived from parent cells labelled with a cyanine dye wherein fluorescence is used to measure changes in levels of cyanine dye.
Claim 3
Dependent Claim
12 words
A method of claim 1 wherein the cells are tissue culture cells.
Claim 4
Dependent Claim
12 words
A method of claim 2 wherein the cells are human tumor cells.
Claim 5
Dependent Claim
28 words
A method of claim 3 wherein the human tumor cells are grown in media containing cancer therapeutic agents so that tumor cell sensitivity to these agents is determined.
Claim 6
Dependent Claim
12 words
A method of claim 2 wherein the cells are white blood cells.
Claim 7
Dependent Claim
16 words
The method claim 2 wherein the cyanine dye is DiSC 14 (5) or DiOC 14 (3).
Claim 8
Dependent Claim
10 words
A method of claim 1 wherein the cells are bacteria.
Claim 9
Dependent Claim
24 words
A method of claim 7 wherein the bacteria are grown in media containing antibacterial agents so that bacterial sensitivity to these agents is determined.
Claim 10
Dependent Claim
17 words
A method of claim 8 wherein the cyanine dye is DiSC 14 (5) or DiOC 14 (3).
Claim 11
Dependent Claim
10 words
A method of claim 1 wherein the cells are yeast.
Claim 12
Dependent Claim
24 words
A method of claim 1 wherein the yeast are grown in media containing antifungal agents so that sensitivity to these agents can be determined.
Claim 13
Dependent Claim
17 words
A method of claim 11 wherein the cyanine dye is DiSC 14 (5) or DiOC 14 (3).
Claim 14
Dependent Claim
13 words
A method of claim 1 wherein the cells are in vivo tissue cells.
Claim 15
Dependent Claim
14 words
A method of claim 13 wherein the in vivo tissue cells are transplanted cells.
Claim 16
Dependent Claim
13 words
A method of claim 14 wherein the transplanted cells are bone marrow cells.
Claim 17
Dependent Claim
15 words
A method of claim 13 wherein the in vivo tissue cells are corneal epithelial cells.
Claim 18
Dependent Claim
17 words
A method of claim 1 wherein the cyanine dye is DiSC 14 (5) or DiOC 14 (3).
Claim 19
Dependent Claim
18 words
A method of claim 1 wherein the cyanine dye absorbs light at a wavelength greater than 680 nm.
Claim 20
Dependent Claim
18 words
A method of claim 16 wherein the cyanine dye absorbs light at a wavelength greater than 680 nm.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-08-21 | 1993-02-20 | 1993-08-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-08-21 | 1997-02-20 | 1997-08-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-08-21 | 2001-02-20 | 2001-08-22 | $7700.00 | Window Closed / Lapsed |