Patent US4859583A successfully refreshed from Google Patents.
US4859583A
Expired / Lapsed
US
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Chemiluminescent immunochemical technique for low molecular weight antigens
Assignee: BP Corp North America Inc
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Filing: 1985-02-25
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Granted: 1989-08-22
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Expires: 2005-02-25
Abstract
Abstract A sample device and method for assaying samples containing members of immunological pairs by chemiluminescence include a first chamber wherein antibody-conjugated reagent antigen labelled with a chemiluminescence moiety is displaced from the antibody by sample antigen. The labelled reagent antigen diffuses into a second chamber, wherein the chemiluminescence moiety reacts with cofactors to produce a light emission proportional to the amount of sample antigen present.
Inventors
- 👤 Michael J. Heller
- 👤 Larry E. Morrison
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 05:33
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; G01N33/58; G01N33/582; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/968; Y10S436/00; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 40
Claim 1
Independent Claim
177 words
A sample device for assaying fluid samples potentially containing an analyte, wherein said analyte includes a member of a liquid-antiligand pair, comprising: a containment vessel, adapted to receive a fluid sample, and containing a reagent, said vessel having a first chamber, a second chamber and cofactor blocking means, wherein said reagent includes a cofactor source and reagent members of a liquid-antiligand pair specific to the analyte of interest, one of said reagent members being restricted in said first chamber, the opposing reagent member having a label agent, said cofactor source providing cofactors which participate in a reaction with said label agent, which reaction is detectable, said first chamber being in fluid communication with said second chamber, said cofactor blocking means interposed between said first and second chambers limiting the presence of said cofactor to said second chamber, allowing said label agent to be detected in said second chamber to the exclusion of said first chamber, said opposing reagent member having a label agent assuming a concentration in said second chamber in relation to the concentration of analyte.
Claim 2
Dependent Claim
177 words
A sample device for assaying fluid samples potentially containing an analyte, wherein said analyte includes a member of a liquid-antiligand pair, comprising: a containment vessel, adapted to receive a fluid sample, and containing a reagent, said vessel having a first chamber, a second chamber and cofactor blocking means, wherein said reagent includes a cofactor source and reagent members of a liquid-antiligand pair specific to the analyte of interest, one of said reagent members being restricted in said first chamber, the opposing reagent member having a label agent, said cofactor source providing cofactors which participate in a reaction with said label agent, which reaction is detectable, said first chamber being in fluid communication with said second chamber, said cofactor blocking means interposed between said first and second chambers limiting the presence of said cofactor to said second chamber, allowing said label agent to be detected in said second chamber to the exclusion of said first chamber, said opposing reagent member having a label agent assuming a concentration in said second chamber in relation to the concentration of analyte.
Claim 3
Dependent Claim
19 words
The sample device of claim 1 wherein said label agent is a chemiluminescence moiety which participates in light-producing reactions.
Claim 4
Dependent Claim
16 words
The sample device of claim 2 further comprising means for detecting light within said second chamber.
Claim 5
Dependent Claim
22 words
The sample device of claim 3 wherein said means for detecting light within said second chamber comprises at least one transparent surface.
Claim 6
Dependent Claim
14 words
The sample device of claim 2 wherein said cofactor source includes a cofactor-generating catalyst.
Claim 7
Dependent Claim
12 words
The sample device of claim 5 wherein the cofactor-generating catalyst is immobilized.
Claim 8
Dependent Claim
13 words
The sample device of claim 2 wherein blocking means is a permeable membrane.
Claim 9
Dependent Claim
15 words
The sample device of claim 7 wherein said blocking means is a cofactor scavenger catalyst.
Claim 10
Dependent Claim
16 words
The sample device of claim 8 wherein said cofactor scavenger catalyst is immobilized on said membrane.
Claim 11
Dependent Claim
22 words
The sample device of claim 9 wherein said scavenger catalyst is immobilized on the side of the membrane within the first chamber.
Claim 12
Dependent Claim
24 words
The sample device of claim 5 wherein the second chamber contains a substrate for producing a cofactor in the presence of the cofactor-generating catalyst.
Claim 13
Dependent Claim
21 words
The sample device of claim 1 wherein the reagent members in the first chamber are conjugated with said opposing reagent members.
Claim 14
Dependent Claim
24 words
The sample device of claim 7 wherein said reagent members in the first chamber are affixed to macromolecules unable to diffuse through said membrane.
Claim 15
Dependent Claim
40 words
The sample device of claim 1 further comprising means for introducing a sample into the first chamber wherein said analyte members are able to enter said first chamber in close proximity to said reagent members restricted to said first chamber.
Claim 16
Dependent Claim
45 words
The sample device of claim 14 wherein said means for introducing a sample comprises a reservoir in fluid communication with said first chamber and a sample membrane separating said reservoir from said first chamber to which reagent members restricted to said first chamber are affixed.
Claim 17
Dependent Claim
20 words
The sample device of claim 4 wherein the cofactor source in the second chamber is immobilized on said transparent surface.
Claim 18
Dependent Claim
24 words
The sample device of claim 4 wherein the transparent surface of the second chamber has immobilized reagent members specific to said labelled reagent member.
Claim 19
Dependent Claim
12 words
The sample device of claim 2 wherein said label agent is peroxidase.
Claim 20
Dependent Claim
13 words
The sample device of claim 5 wherein the cofactor-generating catalyst is glucose oxidase.
Claim 21
Dependent Claim
15 words
The sample device of claim 5 wherein glucose oxidase is immobilized on a transparent surface.
Claim 22
Dependent Claim
12 words
The sample device of claim 8 wherein the scavenger catalyst is catalase.
Claim 23
Dependent Claim
170 words
A sample device for assaying fluid samples potentially containing an analyte, wherein said analyte is a member of an immunological pair, comprising: a containment vessel adapted to receive a fluid sample, and containing a reagent, said vessel having a first chamber, a second chamber, and cofactor blocking means, wherein said reagent includes a cofactor source and reagent members of an immunological pair specific to the analyte of interest, said reagent immunological pair members including one reagent member restricted to said first chamber, the opposing reagent member having a chemiluminescence label agent, said cofactor source providing cofactors which participate in a reaction with said chemiluminescent label agent to produce a detectable light response, said first chamber being in fluid communication with said second chamber, said cofactor blocking means allowing light detection of said chemiluminescent label agent in said second chamber to the exclusion of said first chamber, said opposing reagent member having a chemiluminescent label agent assuming a concentration in said second chamber in relation to the concentration of the analyte.
Claim 24
Dependent Claim
170 words
A sample device for assaying fluid samples potentially containing an analyte, wherein said analyte is a member of an immunological pair, comprising: a containment vessel adapted to receive a fluid sample, and containing a reagent, said vessel having a first chamber, a second chamber, and cofactor blocking means, wherein said reagent includes a cofactor source and reagent members of an immunological pair specific to the analyte of interest, said reagent immunological pair members including one reagent member restricted to said first chamber, the opposing reagent member having a chemiluminescence label agent, said cofactor source providing cofactors which participate in a reaction with said chemiluminescent label agent to produce a detectable light response, said first chamber being in fluid communication with said second chamber, said cofactor blocking means allowing light detection of said chemiluminescent label agent in said second chamber to the exclusion of said first chamber, said opposing reagent member having a chemiluminescent label agent assuming a concentration in said second chamber in relation to the concentration of the analyte.
Claim 25
Dependent Claim
36 words
The sample device of claim 22 further comprising means for introducing sample into the first chamber wherein said sample analyte enters said first chamber in close proximity to said reagent members restricted to said first chamber.
Claim 26
Dependent Claim
44 words
The sample device of claim 23 wherein said means for introducing sample is a sample reservoir in communication with said first chamber, said sample reservoir separated from said first chamber by permeable membrane on which said reagent members within the first chamber are immobilized.
Claim 27
Dependent Claim
14 words
The sample device of claim 22 wherein said blocking means includes a permeable membrane.
Claim 28
Dependent Claim
14 words
The sample device of claim 25 wherein said permeable membrane is a scavenger catalyst.
Claim 29
Independent Claim
195 words
A method for assaying a fluid sample potentially containing an analyte of interest, wherein said analyte is a member of a liquid-antiligand pair, comprising: (a) introducing a fluid sample potentially containing the analyte into a containment vessel containing a reagent and having a first chamber, a second chamber, and cofactor blocking means, wherein said reagent includes a cofactor source and reagent members of a ligand-antiligand pair specific to the analyte of interest, including one reagent member restricted to said first chamber, the opposing reagent member having a label agent, said cofactor source providing cofactors which participate in a reaction with said label agent which reaction produces a detectable response, wherein said analyst may compete with said reagent ligand-antiligand pair and allow said labelled opposing reagent to enter said second chamber, said cofactor blocking means limiting the presence of said cofactor to said second chamber and allowing said label agent to be detected in said second chamber to the exclusion of said first chamber, said opposing reagent member having a label agent assuming a concentration in said second chamber in relation to the concentration of analyte; and, (b) monitoring said second chamber for said label agent.
Claim 30
Independent Claim
195 words
A method for assaying a fluid sample potentially containing an analyte of interest, wherein said analyte is a member of a liquid-antiligand pair, comprising: (a) introducing a fluid sample potentially containing the analyte into a containment vessel containing a reagent and having a first chamber, a second chamber, and cofactor blocking means, wherein said reagent includes a cofactor source and reagent members of a ligand-antiligand pair specific to the analyte of interest, including one reagent member restricted to said first chamber, the opposing reagent member having a label agent, said cofactor source providing cofactors which participate in a reaction with said label agent which reaction produces a detectable response, wherein said analyst may compete with said reagent ligand-antiligand pair and allow said labelled opposing reagent to enter said second chamber, said cofactor blocking means limiting the presence of said cofactor to said second chamber and allowing said label agent to be detected in said second chamber to the exclusion of said first chamber, said opposing reagent member having a label agent assuming a concentration in said second chamber in relation to the concentration of analyte; and, (b) monitoring said second chamber for said label agent.
Claim 31
Dependent Claim
23 words
The method of claim 27 wherein said reagent members of a ligand-antiligand pair are conjugated prior to the introduction of said fluid sample.
Claim 32
Dependent Claim
11 words
The method of claim 27 wherein said label agent is peroxidase.
Claim 33
Dependent Claim
13 words
The method of claim 28 wherein said cofactor source is a cofactor-generating catalyst.
Claim 34
Dependent Claim
17 words
The method of claim 27 wherein cofactor blocking means prevents said cofactor from entering said first chamber.
Claim 35
Dependent Claim
20 words
The method of claim 27 wherein said cofactor blocking means deactivates said cofactor as said cofactor enters said first chamber.
Claim 36
Dependent Claim
17 words
The method of claim 27 wherein said label agent participates in reactions which produce a light emission.
Claim 37
Dependent Claim
25 words
The method of claim 33 wherein said cofactor is selected from the group of cofactors consisting essentially of hydrogen peroxide, luminol, glucose, and glucose oxidase.
Claim 38
Dependent Claim
13 words
The method of claim 27 wherein said blocking means is a light screen.
Claim 39
Dependent Claim
33 words
The method of claim 27 wherein said blocking means is label direction means positioned relative to said first chamber to detect label in said second chamber to the exclusion of said first chamber.
Claim 40
Dependent Claim
25 words
The sample device of claim 7 wherein said cofactor is associated with a macromolecule within said second chamber and is impermeable to said permeable membrane.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-08-21 | 1993-02-20 | 1993-08-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-08-21 | 1997-02-20 | 1997-08-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-08-21 | 2001-02-20 | 2001-08-22 | $7700.00 | Window Closed / Lapsed |