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US4855405A Expired / Lapsed US ⏱️ 0.0 years remaining

Glutamate receptor inhibitor

Assignee: Tokyo Metropolitan Institute of Medical Science
•
Filing: 1987-06-08
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Granted: 1989-08-08
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Expires: 2007-06-08
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Abstract

Abstract A novel glutamate receptor inhibitor obtained from Joro spider venom glands, and its manufacturing method.

Inventors

  • 👤 Masanori Yoshioka
  • 👤 Hidemitsu Hou
  • 👤 Nobufumi Kawai

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-07 23:39
CPC Classifications
A; A61; A61K; A61K38/00; A61K38/16; A61K38/17; A61K38/1767; A01; A01N; A01N63/00; A01N63/10; A01N63/16; A61P; A61P43/00

Claims Analysis

Total Claims: 8
Claim 1
Independent Claim 191 words
An insecticidally effective composition comprising a glutamate receptor inhibitor, said glutamate receptor inhibitor having insecticidal properties when present in a concentration of at least 10 -9 M, said glutamate receptor inhibitor possessing only one single spot at the position of Rf value of substantially 0.20 on a Silica gel 60 thin-layer plate developed with phenol-water mixed solvent 3:1 (V/V), said glutamate receptor inhibitor being an organic compound obtained from Joro spider venom glands and which has the following properties: (a) molecular weight is within the range of 500±200; (b) it is substantially insoluble in ethyl acetate and soluble in water; (c) the activity remains unchanged when heated to 80° C. for 20 minutes in water; (d) the activity remains unchanged when heated to 80° C. for 20 minutes in 0.4 M perchloric acid; (e) it shows in electrophoresis neutral electric charge with use of 0.01 M phosphate buffer (pH 7) and positive electric charge using 0.2 M formate buffer (pH 2.25); (f) it develops color with ninhydrin and fluoresces with fluorescamine; (g) it shows maximum absorption substantially at 270 nm, and minimum absorption substantially at 245 nm in water (pH 6.1).
Claim 2
Dependent Claim 191 words
An insecticidally effective composition comprising a glutamate receptor inhibitor, said glutamate receptor inhibitor having insecticidal properties when present in a concentration of at least 10 -9 M, said glutamate receptor inhibitor possessing only one single spot at the position of Rf value of substantially 0.20 on a Silica gel 60 thin-layer plate developed with phenol-water mixed solvent 3:1 (V/V), said glutamate receptor inhibitor being an organic compound obtained from Joro spider venom glands and which has the following properties: (a) molecular weight is within the range of 500±200; (b) it is substantially insoluble in ethyl acetate and soluble in water; (c) the activity remains unchanged when heated to 80° C. for 20 minutes in water; (d) the activity remains unchanged when heated to 80° C. for 20 minutes in 0.4 M perchloric acid; (e) it shows in electrophoresis neutral electric charge with use of 0.01 M phosphate buffer (pH 7) and positive electric charge using 0.2 M formate buffer (pH 2.25); (f) it develops color with ninhydrin and fluoresces with fluorescamine; (g) it shows maximum absorption substantially at 270 nm, and minimum absorption substantially at 245 nm in water (pH 6.1).
Claim 3
Dependent Claim 30 words
An insecticidal composition comprising an amount of the glutamate receptor inhibitor of claim 1 in a concentration of at least 10 ppm and an agriculturally or horticulturally acceptable carrier therefor.
Claim 4
Dependent Claim 17 words
A method of combatting insects which comprises contacting said insects with an insecticidal composition of claim 2.
Claim 5
Dependent Claim 159 words
A glutamate receptor inhibiting compound obtained from Joro spider venom glands, said compound possessing only one single spot at the position of Rf value of substantially 0.20 on a Silica gel 60 thin-layer plate developed with phenol-water mixed solvent 3:1 (V/V), said compound having the following properties: (a) molecular weight is within the range of 500±200; (b) it is substantially insoluble in ethyl acetate and soluble in water; (c) the activity remains unchanged when heated to 80° C. for 20 minutes in water; (d) the activity remains unchanged when heated to 80° C. for 20 minutes in 0.4 M perchloric acid; (e) it shows in electrophoresis neutral electric charge with use of 0.01 M phosphate buffer (pH 7) and positive electric charge using 0.2 M formate buffer (pH 2.25); (f) it develops color with ninhydrin and fluoresces with fluorescamine; (g) it shows maximum absorption substantially at 270 nm, and minimum absorption substantially at 245 nm in water (pH 6.1).
Claim 6
Dependent Claim 159 words
A glutamate receptor inhibiting compound obtained from Joro spider venom glands, said compound possessing only one single spot at the position of Rf value of substantially 0.20 on a Silica gel 60 thin-layer plate developed with phenol-water mixed solvent 3:1 (V/V), said compound having the following properties: (a) molecular weight is within the range of 500±200; (b) it is substantially insoluble in ethyl acetate and soluble in water; (c) the activity remains unchanged when heated to 80° C. for 20 minutes in water; (d) the activity remains unchanged when heated to 80° C. for 20 minutes in 0.4 M perchloric acid; (e) it shows in electrophoresis neutral electric charge with use of 0.01 M phosphate buffer (pH 7) and positive electric charge using 0.2 M formate buffer (pH 2.25); (f) it develops color with ninhydrin and fluoresces with fluorescamine; (g) it shows maximum absorption substantially at 270 nm, and minimum absorption substantially at 245 nm in water (pH 6.1).
Claim 7
Dependent Claim 33 words
An insecticidal composition of claim 2 comprising an amount of the glutamate receptor inhibitor of claim 8 in a concentration of at least 10 ppm and an agriculturally or horticulturally acceptable carrier therefor.
Claim 8
Dependent Claim 17 words
A method of combatting insects which comprises contacting said insects with an insecticidal composition of claim 1.

References Cited (Backward Citations)

US4452888A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1992-08-07 1993-02-06 1993-08-08 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1996-08-07 1997-02-06 1997-08-08 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2000-08-07 2001-02-06 2001-08-08 $7700.00 Window Closed / Lapsed

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