US4452888A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing a low-molecular weight peptide composition and nutrient agent containing the same
Assignee: Terumo Corp
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Filing: 1981-07-01
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Granted: 1984-06-05
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Expires: 2001-07-01
Abstract
Abstract A low-molecular weight peptide composition mainly based on dipeptides and tripeptides is produced by dispersing protein raw material from any suitable source in water at a concentration of 5 to 20 w/v %, adjusting the pH of the dispersion to 1 to 4 with an acid, adding at least two acid proteases to the dispersion, and permitting enzymatic proteolysis to take place for a desired period of time at a suitable temperature while supressing the formation of free amino acids. Also provided is a nutrient agent comprising the thus produced low-molecular weight peptide composition mainly based on dipeptides and tripeptides wherein the contents of free amino acids and those peptides having a molecular weight of at least 700 are individually 20% by weight or less. The nutrient agent is useful in medical treatment.
Inventors
- 👤 Ken-ichi Yamazaki
- 👤 Shoji Takao
- 👤 Hiroshi Hara
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 12:56
CPC Classifications
A; A23; A23J; A23J3/00; A23J3/30; A23J3/32; A23J3/34; A23L; A23L33/00; A23L33/10; A23L33/17; A23L33/18
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
104 words
A process for producing a low-molecular weight peptide composition comprising dispersing a protein raw material in water at a concentration of from 5 to 20 w/v%, adding an acid to the dispersion in an amount sufficient to adjust the pH of the dispersion to between 1 and 4, adding at least two different acid preteases to the pH adjusted dispersion simultaneously or sequentially, and permitting anzymatic proteolysis to take place for 8 to 72 hours at a temperature of from 25° to 60° C., thereby producing a low-molecular weight peptide composition containing primarily dipeptides and tripeptides while suppressing the formation of free amino acids.
Claim 2
Dependent Claim
104 words
A process for producing a low-molecular weight peptide composition comprising dispersing a protein raw material in water at a concentration of from 5 to 20 w/v%, adding an acid to the dispersion in an amount sufficient to adjust the pH of the dispersion to between 1 and 4, adding at least two different acid preteases to the pH adjusted dispersion simultaneously or sequentially, and permitting anzymatic proteolysis to take place for 8 to 72 hours at a temperature of from 25° to 60° C., thereby producing a low-molecular weight peptide composition containing primarily dipeptides and tripeptides while suppressing the formation of free amino acids.
Claim 3
Dependent Claim
46 words
The process according to claim 1, wherein the free amino acids are present in an amount of no more than 20% by weight and those peptides having a molecular weight of at least 700 are present in an amount of no more than 20% by weight.
Claim 4
Dependent Claim
36 words
The process according to claim 1, wherein the acid proteases are selected from the group consisting of an enzymatic preparation consisting essentially of Aspergillo peptidase A, Pepsin, and an acid protease obtained from the genus Rhizopus.
Claim 5
Dependent Claim
36 words
The process according to claim 2, wherein the acid proteases are selected from the group consisting of an enzymatic preparation consisting essentially of Aspergillo peptidase A, Pepsin, and an acid protease obtained from the genus Rhizopus.
Claim 6
Dependent Claim
35 words
The process according to claim 1, further comprising adding the acid proteases to the pH adjusted dispersion in an amount of at least 1% by weight based on the weight of the protein raw material.
Claim 7
Dependent Claim
35 words
The process according to claim 2, further comprising adding the acid proteases to the pH adjusted dispersion in an amount of at least 1% by weight based on the weight of the protein raw material.
Claim 8
Dependent Claim
31 words
The process according to claim 5, wherein the acid proteases are present in an amount of between 2 and 5% by weight based on the weight of the protein raw material.
Claim 9
Dependent Claim
31 words
The process according to claim 6, wherein the acid proteases are present in an amount of between 2 and 5% by weight based on the weight of the protein raw material.
Claim 10
Dependent Claim
18 words
The process according to claim 1, further comprising adding free amino acid to said low-molecular weight peptide composition.
Claim 11
Dependent Claim
18 words
The process according to claim 2, further comprising adding free amino acid to said low-molecular weight peptide composition.
Claim 12
Dependent Claim
9 words
A product produced by the process of claim 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-06-05 | 1987-12-05 | 1988-06-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-06-05 | 1991-12-05 | 1992-06-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-06-05 | 1995-12-05 | 1996-06-05 | $7700.00 | Window Closed / Lapsed |