US4847201A
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US
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DNA encoding for CSF-1 and accompanying recombinant systems
Assignee: Novartis Vaccines and Diagnostics Inc
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Filing: 1988-02-09
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Granted: 1989-07-11
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Expires: 2008-02-09
Abstract
Abstract A colony stimulating factor, CSF-1, is a lymphokine useful in overcoming the immunosuppression induced by chemotherapy or resulting from other causes. CSF-1 is obtained in usable amounts by recombinant methods, including cloning and expression of the murine and human DNA sequences encoding this protein.
Inventors
- 👤 Ernest S. Kaswasaki
- 👤 Martha B. Ladner
- 👤 Janelle N. Van Arsdell
- 👤 Alice M. Wang
- 👤 Peter Ralph
- 👤 Mazie Y. Coyne
- 👤 Mary K. Warren
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 05:39
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/73; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/53; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/14; Y10S930/145
Claims Analysis
Total Claims: 37
Claim 1
Independent Claim
31 words
An isolated DNA sequence which encodes for human CSF-1 polypeptide or a murine CSF-1 polypeptide, wherein said polypeptide stimulates the formation of primarily macrophage colonies in the in vitro CSF-1 assay.
Claim 2
Dependent Claim
31 words
An isolated DNA sequence which encodes for human CSF-1 polypeptide or a murine CSF-1 polypeptide, wherein said polypeptide stimulates the formation of primarily macrophage colonies in the in vitro CSF-1 assay.
Claim 3
Dependent Claim
10 words
The DNA sequence of claim 1 which encodes human CSF-1.
Claim 4
Dependent Claim
23 words
The DNA sequence of claim 2 wherein the human CSF-1 has an amino acid sequence substantially equivalent to that of native human mCSF-1.
Claim 5
Dependent Claim
48 words
The recombinant DNA sequence of claim 2 wherein the amino acid sequence of the human CSF-1 is related to that of mCSF-1 by the deletion or conservative substitution of one or more amino acids residing between positions 150 and 224 inclusive of mCSF-1 as shown in FIG. 5.
Claim 6
Dependent Claim
54 words
The DNA sequence of claim 2 wherein the human CSF-1 has an amino acid sequence which is related to that of mCSF-1 by the deletion or conservative substitution of one or more of the amino acids at positions 51 and 52 and/or positions 191, 192 and 193 of mCSF-1 as shown in FIG. 5.
Claim 7
Dependent Claim
49 words
The DNA sequence of claim 2 wherein the human CSF-1 has an amino acid sequence which is related to that of mCSF-1 by the deletion or conservative substitution of one or more of the amino acids at positions 15-20 and/or positions 75-84 of mCSF-1 as shown in FIG. 5.
Claim 8
Dependent Claim
36 words
The DNA sequence of claim 2 wherein the human CSF-1 has an amino acid sequence which is related to that of mCSF-1 by the deletion or substitution of the tyrosine residue at position 59 of mCSF-1.
Claim 9
Dependent Claim
37 words
The DNA sequence of claim 2 wherein the human CSF-1 has an amino acid sequence which is selected from the group consisting of mCSF-1, ∇ 158- CSF-1, ∇ 150- CSF-1, gln 52 CSF-1, and asp 59 CSF-1.
Claim 10
Dependent Claim
18 words
The DNA sequence of claim 1 wherein the CSF-1 has the amino acid sequence of native murine CSF-1.
Claim 11
Dependent Claim
21 words
The DNA sequence of claim 2 which encodes a protein of the amino acid sequence encoded in pHCSF-1a or in CSF-17.
Claim 12
Dependent Claim
44 words
A replicative cloning vector which comprises the DNA sequence encoding for a human CSF-1 polypeptide or a murine CSF-1 polypeptide, wherein said polypeptide stimulates the formation of primarily macrophage colonies in the in vitro CSF-1 assay, and a replicon operative in a unicellular organism.
Claim 13
Dependent Claim
44 words
A replicative cloning vector which comprises the DNA sequence encoding for a human CSF-1 polypeptide or a murine CSF-1 polypeptide, wherein said polypeptide stimulates the formation of primarily macrophage colonies in the in vitro CSF-1 assay, and a replicon operative in a unicellular organism.
Claim 14
Dependent Claim
26 words
The vector of claim 11 wherein the sequence encoding CSF-1 encodes a protein which has an amino acid sequence substantially equivalent to that in human mCSF-1.
Claim 15
Dependent Claim
39 words
The vector of claim 12 wherein the sequence encoding CSF-1 encodes a protein which has an amino acid sequence selected from the group consisting of mCSF-1, ∇ 158- CSF-1, ∇ 150- CSF-1, gln 52 CSF-1, and asp 59 CSF-1.
Claim 16
Dependent Claim
23 words
The vector of claim 11 wherein the sequence encoding CSF-1 encodes a protein of the amino acid sequence encoded in pHCSF-1a or pcCSF-17.
Claim 17
Dependent Claim
16 words
An expression system which comprises the DNA of claim 1 operably linked to suitable control sequences.
Claim 18
Dependent Claim
27 words
The expression system of claim 15 wherein the recombinant DNA sequence encodes human CSF-1 which has an amino acid sequence substantially equivalent to that of human mCSF-1.
Claim 19
Dependent Claim
40 words
The expression system of claim 15 wherein the recombinant DNA sequence encodes human CSF-1 which has an amino acid sequence selected from the group consisting of mCSF-1, ∇ 158- CSF-1, ∇ 150- CSF-1, gln 52 CSF-1, and asp 59 CSF-1.
Claim 20
Dependent Claim
17 words
The expression system of claim 15 wherein the recombinant DNA sequence encoding CSF-1 comprises pHCSF-1a or pcCSF-17.
Claim 21
Dependent Claim
17 words
The expression system of claim 15 disposed in a vector capable of replication in suitable host cells.
Claim 22
Dependent Claim
17 words
The expression system of claim 16 disposed in a vector capable of replication in suitable host cells.
Claim 23
Dependent Claim
11 words
Recombinant host cells transformed with the expression system of claim 15.
Claim 24
Dependent Claim
11 words
Recombinant host cells transformed with the expression system of claim 16.
Claim 25
Dependent Claim
23 words
A method of produciang recombinant CSF-1 which comprises culturing the cells of claim 21 under conditions effective for the production of said CSF-1.
Claim 26
Dependent Claim
23 words
A method of producing recombinant CSF-1 which comprises culturing the cells of claim 22 under conditions effective for the production of said CSF-1.
Claim 27
Dependent Claim
11 words
The DNA of claim 2 which is disposed in clone pHCSF-1a.
Claim 28
Dependent Claim
11 words
The DNA of claim 2 which is disposed in clone pcCSF-17.
Claim 29
Dependent Claim
39 words
An isolated DNA sequence which encodes for a human CSF-1 polypeptide wherein the CSF-1 has an N-terminal amino acid sequence comprising: Glu-Glu-Val-Ser-Glu-Tyr-Cys-Ser-His-Met-lle-Gly-Ser-Gly-His-Leu-Gln-Ser-Leu-Gln-Arg-Leu-lle-Asp-Ser-Gln-Met-Glu-Thr-Ser-Cys-Gln-lle-Thr-Phe-Glu-Phe-Val-Asp-Gln-Glu-Gln-Leu and wherein said polypeptide stimulates the formation of primarily macrophage colonies in the in vitro CSF-1 assay.
Claim 30
Dependent Claim
39 words
An isolated DNA sequence which encodes for a human CSF-1 polypeptide wherein the CSF-1 has an N-terminal amino acid sequence comprising: Glu-Glu-Val-Ser-Glu-Tyr-Cys-Ser-His-Met-lle-Gly-Ser-Gly-His-Leu-Gln-Ser-Leu-Gln-Arg-Leu-lle-Asp-Ser-Gln-Met-Glu-Thr-Ser-Cys-Gln-lle-Thr-Phe-Glu-Phe-Val-Asp-Gln-Glu-Gln-Leu and wherein said polypeptide stimulates the formation of primarily macrophage colonies in the in vitro CSF-1 assay.
Claim 31
Dependent Claim
39 words
An isolated DNA sequene which encodes for a murine CSF-1 polypeptide wherein the CSF-1 has an N-terminal amino acid sequence comprisng: Lys-Glu-Val-Ser-Glu-His-Cys-Ser-His-Met-Ile-Gly-Asn-Gly-His-Leu-Lys-Val-Leu-Gln-Gln-Leu-Ile-Asp-Ser-Gln-Met-Glu-Thr-Ser- and wherein said polypeptide stimulates the formation of primarily macrophage colonies in the in vitro CSF-1 assay.
Claim 32
Dependent Claim
39 words
An isolated DNA sequene which encodes for a murine CSF-1 polypeptide wherein the CSF-1 has an N-terminal amino acid sequence comprisng: Lys-Glu-Val-Ser-Glu-His-Cys-Ser-His-Met-Ile-Gly-Asn-Gly-His-Leu-Lys-Val-Leu-Gln-Gln-Leu-Ile-Asp-Ser-Gln-Met-Glu-Thr-Ser- and wherein said polypeptide stimulates the formation of primarily macrophage colonies in the in vitro CSF-1 assay.
Claim 33
Dependent Claim
22 words
A replicative cloning vector which comprises the DNA sequence of claim 27 or 28 and a replicon operative in a unicellular organism.
Claim 34
Dependent Claim
19 words
An expression system which comprises the DNA sequence of claim 27 or 28 operably linked to suitable control sequences.
Claim 35
Dependent Claim
24 words
Recombinant host cells transformed with an expression system which comprises the DNA sequence of claim 27 or 28 operably linked to suitable control sequences.
Claim 36
Dependent Claim
40 words
A method of producing recombinant CSF-1 which comprises culturing recombinant host cells transformed with an expression system which comprises the DNA sequence of claim 23 operably linked to suitable control sequences under conditions effective for the production of said CSF-1.
Claim 37
Dependent Claim
40 words
A method of producing recombinant CSF-1 which comprises culturing recombinant host cells transformed with an expression system which comprises the DNA sequence of claim 28 operably linked to suitable control sequences under conditions effective for the production of said CSF-1.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-07-10 | 1993-01-09 | 1993-07-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-07-10 | 1997-01-09 | 1997-07-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-07-10 | 2001-01-09 | 2001-07-11 | $7700.00 | Window Closed / Lapsed |