Patent DE531946C successfully refreshed from Google Patents.
Patent US2325427A successfully refreshed from Google Patents.
Patent US4838888A successfully refreshed from Google Patents.
US4838888A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Calcification mitigation of implantable bioprostheses
Assignee: Edwards Lifesciences Corp
•
Filing: 1987-04-17
•
Granted: 1989-06-13
•
Expires: 2007-04-17
Abstract
Abstract A process for treating biological tissue prior to implantation to mitigate the calcification of the tissue following implantation comprises incorporating acetylsalicylic acid into the tissue in an amount effective to reduce calcification of the tissue after it is implanted. In accordance with one embodiment, the process involves convalently binding the acetylsalicylic acid to the tissue via a coupling agent.
Inventors
- 👤 Aws S. Nashef
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 21:55
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3683; A61L27/3687; A61F; A61F2/00; A61F2/02; A61F2/24; A61K; A61K35/00; A61K35/12; A61K35/30; A61K35/32; A61K35/34; A61L27/50; A61L2400/00; A61L2400/02; Y; Y10; Y10S; Y10S128/00; Y10S128/08; Y10S623/00; Y10S623/915; Y10S623/918
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
67 words
A process for treating animal biological tissue prior to implantation in an animal to reduce calcification of said tissue following implantation, which comprises: (a) fixing said tissue under tissue-fixing conditions, and (b) contacting said tissue with a solution of acetylsalicylic acid for a time sufficient to covalently bind said acetylsalicylic acid directly to said tissue in an amount effective to reduce calcification of said tissue after implantation.
Claim 2
Dependent Claim
67 words
A process for treating animal biological tissue prior to implantation in an animal to reduce calcification of said tissue following implantation, which comprises: (a) fixing said tissue under tissue-fixing conditions, and (b) contacting said tissue with a solution of acetylsalicylic acid for a time sufficient to covalently bind said acetylsalicylic acid directly to said tissue in an amount effective to reduce calcification of said tissue after implantation.
Claim 3
Dependent Claim
18 words
The process of claim 1, wherein said biological tissue is tendon, ligament, heart valve, dura mater or pericardium.
Claim 4
Dependent Claim
13 words
The process of claim 1, wherein said biological tissue is fixed with glutaraldehyde.
Claim 5
Dependent Claim
18 words
The process of claim 1, wherein the concentration of acetylsalicylic acid is from about 0.05M to about 0.2M.
Claim 6
Dependent Claim
23 words
The process of claim 4, wherein said tissue is contacted with said acetylsalicylic acid solution for about 20 minutes to about 60 minutes.
Claim 7
Dependent Claim
95 words
A process for treating animal biological tissue prior to implantation in an animal to reduce calcification of said tissue following implantation, which comprises: (a) fixing said tissue under tissue-fixing conditions, (b) contacting said fixed tissue with a solution of a coupling agent for a time sufficient to covalently bond said coupling agent to said tissue, and (c) contacting said tissue with a solution of acetylsalicylic acid for a time sufficient to covalently bond said acetylsalicylic acid to said tissue through said coupling agent in an amount effective to reduce calcification of said tissue after implantation.
Claim 8
Dependent Claim
95 words
A process for treating animal biological tissue prior to implantation in an animal to reduce calcification of said tissue following implantation, which comprises: (a) fixing said tissue under tissue-fixing conditions, (b) contacting said fixed tissue with a solution of a coupling agent for a time sufficient to covalently bond said coupling agent to said tissue, and (c) contacting said tissue with a solution of acetylsalicylic acid for a time sufficient to covalently bond said acetylsalicylic acid to said tissue through said coupling agent in an amount effective to reduce calcification of said tissue after implantation.
Claim 9
Dependent Claim
12 words
The process of claim 6, wherein said coupling agent is a diamine.
Claim 10
Dependent Claim
22 words
The process of claim 6, wherein said tissue is contacted with a carbodiimide between steps (b) and (c) and after step (c).
Claim 11
Dependent Claim
34 words
The process of claim 7, wherein said diamine has the formula R--(NH 2 ) 2 , wherein R is an aliphatic group having a straight, branched or cyclic chain or is an aromatic group.
Claim 12
Dependent Claim
10 words
The process of claim 9, wherein said diamine is ethylenediamine.
Claim 13
Dependent Claim
19 words
The process of claim 6, wherein said biological tissue is a tendon, ligament, heart valve, dura mater or pericardium.
Claim 14
Dependent Claim
13 words
The process of claim 6, wherein said biological tissue is fixed with glutaraldehyde.
Claim 15
Dependent Claim
20 words
The process of claim 6, wherein the concentration of coupling agent in solution is from about 0.01M to about 0.5M.
Claim 16
Dependent Claim
18 words
The process of claim 6, wherein the concentration of acetylsalicylic acid is from about 0.05M to about 0.2M.
Claim 17
Dependent Claim
26 words
The process of claim 14, wherein the tissue is contacted with said acetylsalicylic acid solution for a time of about 20 minutes to about 60 minutes.
Claim 18
Dependent Claim
33 words
The process of claim 6, wherein said tissue is maintained at a pH of from about 4 to about 5 as it is contacted with said coupling agent and with said acetylsalicylic acid.
Claim 19
Dependent Claim
66 words
A process for treating animal biological tissue prior to implantation in an animal to reduce calcification of said tissue following implantation, which comprises: (a) fixing said tissue under tissue fixing conditions, and (b) contacting said tissue with a solution of acetylsalicylic acid for a time sufficient to impregnate said acetylsalicylic acid in said tissue in an amount effective to reduce calcification of said tissue after implantation.
Claim 20
Dependent Claim
66 words
A process for treating animal biological tissue prior to implantation in an animal to reduce calcification of said tissue following implantation, which comprises: (a) fixing said tissue under tissue fixing conditions, and (b) contacting said tissue with a solution of acetylsalicylic acid for a time sufficient to impregnate said acetylsalicylic acid in said tissue in an amount effective to reduce calcification of said tissue after implantation.
Claim 21
Dependent Claim
32 words
Biological tissue having a reduced tendency toward calcification after implantation in an animal, said tissue being covalently bonded to acetylsalicyclic acid in accordance with the process of claim 1 or claim 6.
References Cited (Backward Citations)
US3625745A
Cited
US3975350A
Cited
US4304591A
Cited
US4396716A
Cited
US4378224A
Cited
US4323358A
Cited
US4648881A
Cited
US4481009A
Cited
WO1984001879A1
Cited
US4553974A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-06-12 | 1992-12-12 | 1993-06-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-06-12 | 1996-12-12 | 1997-06-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-06-12 | 2000-12-12 | 2001-06-13 | $7700.00 | Window Closed / Lapsed |