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US4837312A
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Chelator-functionalized nucleosides and nucleotides and methods for making same
Assignee: California Institute of Technology
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Filing: 1985-01-25
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Granted: 1989-06-06
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Expires: 2005-01-25
Abstract
Abstract Nucleosides and nucleotides functionalized with a metal chelator, and methods of synthesizing such nucleosides and nucleotides are described. These compounds are useful, for example, as constituents of nucleic acid probes for diagnostic and therapeutic purposes.
Inventors
- 👤 Peter B. Dervan
- 👤 Geoffrey B. Dreyer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 21:01
CPC Classifications
C; C07; C07H; C07H23/00; C07H21/00
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
168 words
A compound of structure: ##STR2## where Z is hydrogen or hydroxy; Y is NHR 1 , W where w is a hydroxyl protecting group or ##STR3## T is hydrogen or hydroxyl or ##STR4## where Q is OR 1 , R 1 or NHR 1 , or ##STR5## where V is OR 1 , R 1 or NHR 1 and where U is a polyribonucleotide or polydeoxyribonucleotide sequence; and where X is a nucleoside base selected from the group consisting of ##STR6## where R 1 is a thethered metal chelator said metal capable of reducing dioxygen; provided, however, that when Y is ##STR7## or NHR 1 , X is a nucleoside base selected from the group consisting of tethered metal chelator functionalized nucleoside bases guanine, adenine, cytosine, uracil and thymine, provided that when X is thymine, T is hydrogen; and provided that when T is ##STR8## X is a nucleoside base selected from the group consisting of tethered metal chelator functionalized nucleoside bases guanine, adenine, cytosine, uracil and thymine.
Claim 2
Dependent Claim
168 words
A compound of structure: ##STR2## where Z is hydrogen or hydroxy; Y is NHR 1 , W where w is a hydroxyl protecting group or ##STR3## T is hydrogen or hydroxyl or ##STR4## where Q is OR 1 , R 1 or NHR 1 , or ##STR5## where V is OR 1 , R 1 or NHR 1 and where U is a polyribonucleotide or polydeoxyribonucleotide sequence; and where X is a nucleoside base selected from the group consisting of ##STR6## where R 1 is a thethered metal chelator said metal capable of reducing dioxygen; provided, however, that when Y is ##STR7## or NHR 1 , X is a nucleoside base selected from the group consisting of tethered metal chelator functionalized nucleoside bases guanine, adenine, cytosine, uracil and thymine, provided that when X is thymine, T is hydrogen; and provided that when T is ##STR8## X is a nucleoside base selected from the group consisting of tethered metal chelator functionalized nucleoside bases guanine, adenine, cytosine, uracil and thymine.
Claim 3
Dependent Claim
53 words
A compound of structure ##STR9## where Z is hydrogen or hydroxyl; Y is hydroxyl or W where W is a hydroxyl-protecting group; T is hydroxyl and X is a nucleoside base selected from the group consisting of ##STR10## and where R 1 is a tethered metal chelator said metal capable of reducing dioxygen.
Claim 4
Dependent Claim
53 words
A compound of structure ##STR9## where Z is hydrogen or hydroxyl; Y is hydroxyl or W where W is a hydroxyl-protecting group; T is hydroxyl and X is a nucleoside base selected from the group consisting of ##STR10## and where R 1 is a tethered metal chelator said metal capable of reducing dioxygen.
Claim 5
Dependent Claim
16 words
The compound according to claim 1 or 2 wherein the hydroxyl protecting group is dimethoxytrityl chloride.
Claim 6
Dependent Claim
25 words
The compound according to claim 1 or 2 wherein said tether is a hydrocarbon-amide linkage and said metal chelator is a polyamino carboxylic acid chelator.
Claim 7
Dependent Claim
18 words
The compound according to claim 4 wherein said tether is the structure: ##STR11## and said chelator is EDTA.
Claim 8
Dependent Claim
23 words
The compound according to claim 4 wherein the metal chelator is selected from the group consisting of EDTA, DCTA, DTPA, EDDHA and HEEDTA.
Claim 9
Dependent Claim
6 words
The chelator-functionalized nucleoside of structure: ##STR12##
Claim 10
Dependent Claim
6 words
The chelator-functionalized nucleoside of structure: ##STR12##
Claim 11
Dependent Claim
6 words
The chelator-functionalized nucleoside of structure: ##STR13##
Claim 12
Dependent Claim
6 words
The chelator-functionalized nucleoside of structure: ##STR13##
References Cited (Backward Citations)
US4529796A
Cited
US4665184A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-06-05 | 1992-12-05 | 1993-06-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-06-05 | 1996-12-05 | 1997-06-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-06-05 | 2000-12-05 | 2001-06-06 | $7700.00 | Window Closed / Lapsed |