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US4833080A
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Regulation of eucaryotic gene expression
Assignee: Harvard University
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Filing: 1985-12-12
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Granted: 1989-05-23
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Expires: 2005-12-12
Abstract
Abstract Regulation of eucaryotic gene expression is controlled by procaryotic peptides. The peptides recognize specific DNA sequences present in the gene, which may be derived from procaryotic genes, and either activate or repress gene transcription. Hybrid procaryotic peptides may be used containing both repressor and activator peptides.
Inventors
- 👤 Roger Brent
- 👤 Mark S. Ptashne
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-10 01:51
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C07; C07K; C07K14/00; C07K14/195; C07K14/24; C07K14/245; C07K14/37; C07K14/39; C07K2319/00; C07K2319/80; C07K2319/81
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
65 words
A method of regulating the expression of a gene in a eucaryotic cell, said method comprising: providing in said eucaryotic cell, a peptide, derived from or substantially similar to a peptide of a procaryotic cell able to bind to DNA upstream from or within said gene, the amount of said peptide being sufficient to bind to said gene and thereby control expression of said gene.
Claim 2
Independent Claim
65 words
A method of regulating the expression of a gene in a eucaryotic cell, said method comprising: providing in said eucaryotic cell, a peptide, derived from or substantially similar to a peptide of a procaryotic cell able to bind to DNA upstream from or within said gene, the amount of said peptide being sufficient to bind to said gene and thereby control expression of said gene.
Claim 3
Dependent Claim
17 words
The method of claim 1 wherein said peptide is substantially similar to a peptide of E. coli.
Claim 4
Dependent Claim
20 words
The method of claim 2 wherein said peptide is the product of the lexA, lacI, trpR, or Lambda cI genes.
Claim 5
Dependent Claim
12 words
The method of claim 2 wherein said peptide is a fusion peptide.
Claim 6
Dependent Claim
16 words
The method of claim 4 wherein said fusion peptide is the product of a gene fusion.
Claim 7
Dependent Claim
24 words
The method of claim 5 wherein said gene fusion comprises the fusion of GAL4 with the lexA, lacI, trpR, or Lambda cI repressor genes.
Claim 8
Dependent Claim
15 words
The method of claim 1 wherein said peptide is encoded by a plasmid borne gene.
Claim 9
Dependent Claim
17 words
The method of claim 1 wherein said peptide is present in the cytoplasm of said eucaryotic cell.
Claim 10
Dependent Claim
21 words
The method of claim 1 wherein said peptide is encoded by a gene integrated into a chromosome of said eucaryotic cell.
Claim 11
Dependent Claim
27 words
The method of claim 1 wherein said method further comprises regulating the expression of said peptide and thence the expression of said gene in said eucaryotic cell.
Claim 12
Dependent Claim
25 words
The method of claim 10 wherein said regulation of said peptide is thermal, chemical, or by the product of another gene within said eucaryotic cell.
Claim 13
Dependent Claim
18 words
The method of claim 1 wherein said gene in said eucaryotic cell comprises a procaryotic gene operator sequence.
Claim 14
Dependent Claim
26 words
The method of claim 12 wherein said operator is the operator sequence to which the product of the lexA, lacI, trpR, or Lambda cI gene binds.
Claim 15
Dependent Claim
44 words
The method of claim 1 wherein said eucaryotic cell comprises a second peptide, derived from or substantially similar to a second peptide of a procaryotic cell able to bind to DNA upstream from or within said cell, and thereby control expression of said gene.
Claim 16
Dependent Claim
20 words
The method of claim 14 wherein said peptide is a repressor peptide and said second peptide is an activator peptide.
Claim 17
Dependent Claim
25 words
The method of claim 15 wherein said peptide and said second peptide compete for a region on said DNA upstream from or within said gene.
Claim 18
Dependent Claim
37 words
The method of claim 16 wherein said peptide or said second peptide may be inactivated and thereby allow said remaining peptide or said remaining second peptide to bind to said DNA upstream from or within said gene.
Claim 19
Dependent Claim
22 words
The method of claim 17 wherein said inactivation is thermal, chemical, or by the product of another gene within said eucaryotic cell.
Claim 20
Dependent Claim
32 words
A hybrid gene comprising DNA encoding a specific DNA binding procaryotic peptide and DNA encoding a gene activating eucaryotic peptide, said gene activating peptide being effective to enhance transcription of adjacent DNA.
Claim 21
Dependent Claim
32 words
A hybrid gene comprising DNA encoding a specific DNA binding procaryotic peptide and DNA encoding a gene activating eucaryotic peptide, said gene activating peptide being effective to enhance transcription of adjacent DNA.
Claim 22
Dependent Claim
23 words
The hybrid gene of claim 19 wherein said specific binding peptide is a segment of a first protein derived from a procaryotic cell.
Claim 23
Dependent Claim
20 words
The hybrid gene of claim 20 wherein said first protein represses the transcription of a gene within said procaryotic cell.
Claim 24
Dependent Claim
13 words
The hybrid gene of claim 21 wherein said procaryotic cell is E. coli.
Claim 25
Dependent Claim
22 words
The hybrid gene of claim 22 wherein said first protein is the product of the lexA, lacI, trpR, or Lambda cI genes.
Claim 26
Dependent Claim
23 words
The hybrid gene of claim 19 wherein said gene activating peptide is a segment of a second protein derived from a eucaryotic protein.
Claim 27
Dependent Claim
20 words
The hybrid gene of claim 24 wherein said second protein activates the transcription of a gene in said eucaryotic cell.
Claim 28
Dependent Claim
12 words
The hybrid gene of claim 25 wherein said eucaryotic cell is yeast.
Claim 29
Dependent Claim
18 words
The hybrid gene of claim 26 wherein said gene activating peptide is the product of the GAL4 gene.
Claim 30
Dependent Claim
36 words
A eucaryotic cell comprising a peptide derived from or substantially similar to a peptide of a procaryotic cell able to bind to DNA upstream from or within said gene, and thereby control expression of said gene.
Claim 31
Dependent Claim
36 words
A eucaryotic cell comprising a peptide derived from or substantially similar to a peptide of a procaryotic cell able to bind to DNA upstream from or within said gene, and thereby control expression of said gene.
Claim 32
Dependent Claim
22 words
A eucaryotic cell as claimed in claim 28 in which said peptide represses the transcription of a gene in said eucaryotic cell.
Claim 33
Dependent Claim
12 words
The eucaryotic cell of claim 29 wherein said eucaryotic cell is yeast.
Claim 34
Dependent Claim
22 words
The eucaryotic cell of claim 30 wherein said procaryotic protein is derived from or substantially similar to a protein in E. coli.
Claim 35
Dependent Claim
22 words
The eucaryotic cell of claim 31 wherein said procaryotic protein is the product of the lexA, lacI, trpR, or Lambda cI genes.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-05-22 | 1992-11-21 | 1993-05-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-05-22 | 1996-11-21 | 1997-05-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-05-22 | 2000-11-21 | 2001-05-23 | $7700.00 | Window Closed / Lapsed |