Patent US4829000A successfully refreshed from Google Patents.
US4829000A
Expired / Lapsed
US
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Reconstituted basement membrane complex with biological activity
Assignee: DEPARTMENT OF HEALTH AND HUMAN SERVICES United States, THE, Secretary of
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Filing: 1986-05-27
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Granted: 1989-05-09
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Expires: 2006-05-27
Abstract
Abstract The present invention discloses a biologically active basement membrane composition. When polymerized under physiological conditions, the composition forms gel-like structures whose ultrastructure resembles interconnected thin sheets of the lamina densa zone of basement membrane. The major components of the composition include laminin, type IV collagen, heparin sulfate proteoglycan, entactin and nidogen. These components polymerize in constant proportions when redissolved and allowed to reconstitute. Molecular sieve studies on the soluble extract demonstrate that laminin, entactin and nidogen are associated in a large but dissociable complex. The reconstituted matrix is biologically active and stimulates the growth and differentiation of a variety of cells, including epithelial cells, nerve cells, hair follicles and the like. The reconstituted matrix can also be used for determining metastatic potential of tumor cells and for isolating metastatic tumor cells.
Inventors
- 👤 Hynda K. Kleinman
- 👤 George R. Martin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 11:37
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0018; A; A61; A61K; A61K35/00; A61K35/12; A61K35/48; A61K35/50; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5008; G01N33/5014; G01N33/5017; C12N2500/00; C12N2500/70; C12N2500/80; C12N2500/84; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
80 words
In a cell culture composition promoting the growth and differentiation of neurite and epithelial cells comprising a cell innoculum and a cell growth media, the improvement comprising the addition of a protein extract in a concentration of at least 3.7 mg/ml containing in parts by weight about 60-85% laminin, 5-30% collagen IV, 1-10% nidogen, 1-10% heparan sulfate proteoglycan and 1-5% entactin; said protein extract polymerizing into a rigid stable gel in at least 20 minutes on heating at 24°-37° C.
Claim 2
Dependent Claim
80 words
In a cell culture composition promoting the growth and differentiation of neurite and epithelial cells comprising a cell innoculum and a cell growth media, the improvement comprising the addition of a protein extract in a concentration of at least 3.7 mg/ml containing in parts by weight about 60-85% laminin, 5-30% collagen IV, 1-10% nidogen, 1-10% heparan sulfate proteoglycan and 1-5% entactin; said protein extract polymerizing into a rigid stable gel in at least 20 minutes on heating at 24°-37° C.
Claim 3
Dependent Claim
13 words
The composition of claim 1 wherein said extract is derived from Engelbreth-Holm-Swarm tumor.
Claim 4
Dependent Claim
13 words
The composition of claim 1 wherein said extract is derived from human placenta.
Claim 5
Dependent Claim
12 words
The composition of claim 3 having a three dimensional matrix upon polymerization.
Claim 6
Dependent Claim
191 words
A biologically active extract for promoting cell growth and differentiation derived from Engelberth-Holm-Swarm tumor by a process carried out at about 4° C. comprising the sequential steps of: (a) homogenizing the tumor multiple times in a suitable first buffer containing at least 3.4M salt and discarding the soluble fraction after each homogenization so as to remove undesirable components; (b) extracting the residual tumor in a suitable urea buffer containing at leat 2M urea keeping the extract stirred for about 16-18 hours; (c) separating the extract form step (b) and saving the extract while repeating step (b) with the residual tumor fraction and again saving the extract resulting therefrom; (d) combining the extracts from step (c) and dialyzing against a suitable sterilizing buffer with multiple changes of the dialyzing buffer; (e) further dialyzing against a suitable buffer not containing a sterilizing component; (f) recovering a dialysate from step (e); and (g) polymerizing the dialysate at 24°-32° C. for at least 20 minutes, said dialysate in a concentration of at least 3.7 mg/ml containing in parts by weight about 60-85% laminin, 5-30% collagen IV, 1-10% nidogen, 1-101% heparin sulfate proteoglycan and 1-5% entactin.
Claim 7
Dependent Claim
191 words
A biologically active extract for promoting cell growth and differentiation derived from Engelberth-Holm-Swarm tumor by a process carried out at about 4° C. comprising the sequential steps of: (a) homogenizing the tumor multiple times in a suitable first buffer containing at least 3.4M salt and discarding the soluble fraction after each homogenization so as to remove undesirable components; (b) extracting the residual tumor in a suitable urea buffer containing at leat 2M urea keeping the extract stirred for about 16-18 hours; (c) separating the extract form step (b) and saving the extract while repeating step (b) with the residual tumor fraction and again saving the extract resulting therefrom; (d) combining the extracts from step (c) and dialyzing against a suitable sterilizing buffer with multiple changes of the dialyzing buffer; (e) further dialyzing against a suitable buffer not containing a sterilizing component; (f) recovering a dialysate from step (e); and (g) polymerizing the dialysate at 24°-32° C. for at least 20 minutes, said dialysate in a concentration of at least 3.7 mg/ml containing in parts by weight about 60-85% laminin, 5-30% collagen IV, 1-10% nidogen, 1-101% heparin sulfate proteoglycan and 1-5% entactin.
Claim 8
Dependent Claim
20 words
The extract of claim 5 wherein the first buffer is a 3.4M NaCl buffer having a pH of about 7.4.
Claim 9
Dependent Claim
20 words
The extract of claim 5 wherein the urea buffer is a 2M urea buffer having a pH of about 7.4.
Claim 10
Dependent Claim
20 words
The extract of claim 5 wherein the sterilizing buffer is Eagle's minimal essential medium containing a sterilizing amount of chloroform.
Claim 11
Dependent Claim
12 words
The extract of claim 5 supporting growth and differentiation of epithelial cells.
Claim 12
Dependent Claim
12 words
The extract of claim 5 supporting growth and differentiation of neurtic cells.
Claim 13
Dependent Claim
195 words
A biologically active extract for promoting cell growth and differentiation derived from human placental tissue by a process carried out at about 4° C. comprising the sequential steps of: (a) homogenizing placental tissue multiple times in a suitable first buffer containing at least 3.4M salt and discarding the soluble fraction after each homogenization so as to remove undesirable components; (b) extracting the residual tissue in a suitable urea buffer containing at least 2M urea and keeping the extract stirred for about 16-18 hours; (c) separating the extract from step (b) and saving the extract while repeating step (b) with the residual tissue fraction and against saving the extract resulting therefrom; (d) combining the extracts from step (c) and transferring to a heparin affinity column to allow adsorption and then eluting the bound material with a salt solution of greater than physiological concentration; (e) dialyzing the eluate against a sterilizing buffer with multiple changes of the sterilizing buffer; (f) further dialyzing against a suitable buffer not containing a sterilizing component; (g) recovering a dialysate from step (f); and (h) polymerizing the dialysate at 24°-37° C. for at least 20 minutes, said dialysate being a protein extract.
Claim 14
Dependent Claim
195 words
A biologically active extract for promoting cell growth and differentiation derived from human placental tissue by a process carried out at about 4° C. comprising the sequential steps of: (a) homogenizing placental tissue multiple times in a suitable first buffer containing at least 3.4M salt and discarding the soluble fraction after each homogenization so as to remove undesirable components; (b) extracting the residual tissue in a suitable urea buffer containing at least 2M urea and keeping the extract stirred for about 16-18 hours; (c) separating the extract from step (b) and saving the extract while repeating step (b) with the residual tissue fraction and against saving the extract resulting therefrom; (d) combining the extracts from step (c) and transferring to a heparin affinity column to allow adsorption and then eluting the bound material with a salt solution of greater than physiological concentration; (e) dialyzing the eluate against a sterilizing buffer with multiple changes of the sterilizing buffer; (f) further dialyzing against a suitable buffer not containing a sterilizing component; (g) recovering a dialysate from step (f); and (h) polymerizing the dialysate at 24°-37° C. for at least 20 minutes, said dialysate being a protein extract.
References Cited (Backward Citations)
US4280954A
Cited
US4642292A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1992-05-08 | 1992-11-07 | 1993-05-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-05-08 | 1996-11-07 | 1997-05-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-05-08 | 2000-11-07 | 2001-05-09 | $7700.00 | Window Closed / Lapsed |