Patent US2314499A successfully refreshed from Google Patents.
Patent US4826771A successfully refreshed from Google Patents.
US4826771A
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US
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Procedure to be performed in conjunction with protein blotting or nucleic acid blotting
Assignee: Pfizer Health AB
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Filing: 1986-10-08
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Granted: 1989-05-02
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Expires: 2006-10-08
Abstract
Abstract Procedure to be performed in conjunction with protein blotting or nucleic acid blotting wherein the matrix to which said components have been transferred is contacted with reagent and wash solutions in a rotary drum.
Inventors
- 👤 Gosta Eggertsen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 17:21
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; C; C12; C12Q; C12Q1/00; C12Q1/68; G01N33/68; Y; Y10; Y10T; Y10T436/00; Y10T436/11; Y10T436/111666
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
177 words
In a method for processing at least one paper strip containing at least one blotted substance selected from the group consisting of proteins and nucleic acids, which method includes treating said paper strip and blotted substance with a treating liquid selected from the group consisting of a wash liquid and a reagent liquid, the improvement which consists essentially of the steps of (i) placing at least one paper strip containing at least one of said blotted substances against the interior wall surface of a cylindrical rotary drum that is disposed with its axis in a horizontal position, said at least one paper strip being arranged on the drum wall surface so as to extend in the rotational direction of the drum, (ii) introducing a treating liquid into the interior of the drum after step (i), and (iii) rotating said drum about its axis after steps (i) and (ii) so as to bring said treating liquid into contact with said at least one paper strip, the lateral flow of said treating liquid being unrestricted within said rotary drum.
Claim 2
Dependent Claim
177 words
In a method for processing at least one paper strip containing at least one blotted substance selected from the group consisting of proteins and nucleic acids, which method includes treating said paper strip and blotted substance with a treating liquid selected from the group consisting of a wash liquid and a reagent liquid, the improvement which consists essentially of the steps of (i) placing at least one paper strip containing at least one of said blotted substances against the interior wall surface of a cylindrical rotary drum that is disposed with its axis in a horizontal position, said at least one paper strip being arranged on the drum wall surface so as to extend in the rotational direction of the drum, (ii) introducing a treating liquid into the interior of the drum after step (i), and (iii) rotating said drum about its axis after steps (i) and (ii) so as to bring said treating liquid into contact with said at least one paper strip, the lateral flow of said treating liquid being unrestricted within said rotary drum.
Claim 3
Dependent Claim
21 words
A method according to claim 1 wherein less than 50% of the internal drum volume is filled with said treating liquid.
Claim 4
Dependent Claim
19 words
A method according to claim 1 wherein 5-10% of the internal drum volume is filled with said treating liquid.
Claim 5
Dependent Claim
11 words
A method according to claim 1 wherein said strip is paper.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-05-01 | 1992-10-31 | 1993-05-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-05-01 | 1996-10-31 | 1997-05-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-05-01 | 2000-10-31 | 2001-05-02 | $7700.00 | Window Closed / Lapsed |