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US4822470A
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Method of and apparatus for cell poration and cell fusion using radiofrequency electrical pulses
Assignee: Baylor College of Medicine
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Filing: 1987-10-09
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Granted: 1989-04-18
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Expires: 2007-10-09
Abstract
Abstract Disclosed are an apparatus and a method for the poration and fusion of cells using radiofrequency electrical pulses. The electrodes of the apparatus can be hand held or part of integrated equipment with special containers for cells. The electrodes, which are positioned equal distance from each other, are attached to a power function generator. The power function generator can apply a continuous AC electrical field and/or a pulsed radiofrequency electrical field across the electrodes. The alternating electrical field induces cell dielectrophoresis. The pulsed radiofrequency electrical field porates or fuses the cells. The method can be used to fuse or porate a variety of cells including erythrocyte ghosts, liposomes, vesicles, isolated cells and cultured cells. During the poration or fusions a variety of chemical agents including antibodies, proteins, drugs, molecular probes, hormones, growth factors, DNA, RNA, enzymes, organic chemicals and inorganic chemicals can be introduced into these cells. The method can also be used to produce new species and to make hybridoma cells which produce monoclonal antibodies.
Inventors
- 👤 Donald C. Chang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 18:35
CPC Classifications
C; C12; C12M; C12M35/00; C12M35/02; C12M35/04; C12N; C12N13/00; C12N15/00; C12N15/09; C12N15/87
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
37 words
A method for poration of biological particles, comprising the steps of: placing a plurality of biological particles in solution between electrodes; and applying a pulsed radiofrequency oscillating electrical field with sufficient strength to cause portition to occur.
Claim 2
Independent Claim
37 words
A method for poration of biological particles, comprising the steps of: placing a plurality of biological particles in solution between electrodes; and applying a pulsed radiofrequency oscillating electrical field with sufficient strength to cause portition to occur.
Claim 3
Dependent Claim
14 words
The method of claim 1, wherein said biological particles are suspended cells in solution.
Claim 4
Dependent Claim
15 words
The method of claim 1, wherein said biological particles are attached cells in cell cultures.
Claim 5
Dependent Claim
44 words
The method of claim 1, wherein said pulsed radiofrequency field includes a frequency range of about 50 KHz to 500 MHz, a pulse width range of about 1 μsec to 10 msec, and a pulse amplitude range of about 1 KV/cm to 15 KV/cm.
Claim 6
Dependent Claim
37 words
The method of claim 1, wherein said pulsed radiofrequency oscillating field includes a frequency of about 0.1 to 10 MHz; a pulse width of about 20 to 200 μsec and a pulse amplitude of about 5 KV/cm.
Claim 7
Dependent Claim
26 words
The method of claim 1, wherein said frequency of said pulsed radiofrequency oscillating field is sufficient to cause the membrane of the biological particles to resonate.
Claim 8
Dependent Claim
14 words
The method of claim 1, wherein said pulsed radiofrequency oscillating field includes multifrequency components.
Claim 9
Dependent Claim
23 words
The method of claim 1, wherein said biological particles are selected from the group consisting of biological cells, vesicles, erythrocyte ghosts and liposomes.
Claim 10
Independent Claim
42 words
A method for fusing biological particles, comprising the steps of: placing a plurality of biological particles in solution between electrodes; positioning said particles in close proximity; and fusing said biological particles by applying a pulsed radiofrequency oscillating electrical field across said electrodes.
Claim 11
Independent Claim
42 words
A method for fusing biological particles, comprising the steps of: placing a plurality of biological particles in solution between electrodes; positioning said particles in close proximity; and fusing said biological particles by applying a pulsed radiofrequency oscillating electrical field across said electrodes.
Claim 12
Dependent Claim
24 words
The method of claim 9, wherein said positioning step includes placing suspended biological particles in a container which allows said biological particles to congregate.
Claim 13
Dependent Claim
25 words
The method of claim 9, wherein said positioning step includes: applying an alternating electrical field across said electrodes for bringing said particles into close proximity.
Claim 14
Dependent Claim
17 words
The method of claim 11, wherein said applying an alternating electrical field occurs before said porating step.
Claim 15
Dependent Claim
17 words
The method of claim 11, wherein said applying an alternating electrical field occurs after said porating step.
Claim 16
Dependent Claim
19 words
The method of claim 11, wherein said applying an alternating electrical field occurs before and after said porating step.
Claim 17
Dependent Claim
30 words
The method of claim 9, wherein said biological particles include a target cell and a particle preloaded with chemical agents or molecules for introducing chemical agents and molecules into cells.
Claim 18
Dependent Claim
31 words
The method of claim 15, wherein said particle preloaded with said chemical agents or molecules is selected from the group consisting of erythrocyte ghosts, liposomes, vesicles, isolated cells and cultured cells.
Claim 19
Dependent Claim
34 words
The method of claim 15, wherein said chemical agents and molecules are selected from the group consisting of antibodies, proteins, drugs, molecular probes, hormones, growth factors, DNA, RNA, enzymes, organic chemicals and inorganic chemicals.
Claim 20
Dependent Claim
17 words
The method of claim 9, wherein said biological particles include cells from at least two different species.
Claim 21
Dependent Claim
20 words
The method of claim 9, wherein said biological particles includes an antibody producing cell and a continuously dividing cancer cell.
Claim 22
Independent Claim
17 words
The method of claims 9, wherein said biological particles includes hyperimmunized mouse spleen cells and myeloma cells.
Claim 23
Dependent Claim
17 words
The method of claim 9, wherein said biological particles includes cells of at least two different sizes.
Claim 24
Independent Claim
185 words
A method for fusing biological particles, comprising the steps of: placing a plurality of biological particles in solution between electrodes, wherein said biological particles are selected from the group consisting of biological cells, vesicles, erythrocyte ghosts and liposomes; providing a low amplitude alternating electrical field across said electrodes for bringing said particles into close proximity by dielectrophoresis, wherein said electrical field has a frequency range of about 60 Hz to about 10 mega Hz and a field strength of about 100 to 400 V/cm.; applying a pulsed multifrequency radiofrequency oscillating electrical field across said electrodes for porating said particles, wherein said radiofrequency oscillating electrical field includes a frequency range of about 50 KHz to 500 KHz; a pulse width range of about 1 μsec to 10 msec, and a pulse amplitude range of about 1 KV/cm to 15 KV/cm; and providing an alternating electrical field across said electrodes for bringing said particles into close proximity for fusion, wherein said electrical field has a frequency range of about 60 Hz to about 10 mega Hz and a field strength of about 100 V/cm to 400 V/cm.
Claim 25
Independent Claim
185 words
A method for fusing biological particles, comprising the steps of: placing a plurality of biological particles in solution between electrodes, wherein said biological particles are selected from the group consisting of biological cells, vesicles, erythrocyte ghosts and liposomes; providing a low amplitude alternating electrical field across said electrodes for bringing said particles into close proximity by dielectrophoresis, wherein said electrical field has a frequency range of about 60 Hz to about 10 mega Hz and a field strength of about 100 to 400 V/cm.; applying a pulsed multifrequency radiofrequency oscillating electrical field across said electrodes for porating said particles, wherein said radiofrequency oscillating electrical field includes a frequency range of about 50 KHz to 500 KHz; a pulse width range of about 1 μsec to 10 msec, and a pulse amplitude range of about 1 KV/cm to 15 KV/cm; and providing an alternating electrical field across said electrodes for bringing said particles into close proximity for fusion, wherein said electrical field has a frequency range of about 60 Hz to about 10 mega Hz and a field strength of about 100 V/cm to 400 V/cm.
Claim 26
Dependent Claim
71 words
As a composition of matter, a hybridoma cell made by a method for fusing antibody producing cells with continuously dividing cancer cells, comprising the steps of: suspending antibody producing cells and cancer cells in solution between electrodes; providing an alternating electrical field wherein said electric field forms pearl chains by allowing the cells to move dielectrophoretically; and forming said hybridoma cell by applying a pulsed radiofrequency oscillating field across said electrodes.
Claim 27
Dependent Claim
71 words
As a composition of matter, a hybridoma cell made by a method for fusing antibody producing cells with continuously dividing cancer cells, comprising the steps of: suspending antibody producing cells and cancer cells in solution between electrodes; providing an alternating electrical field wherein said electric field forms pearl chains by allowing the cells to move dielectrophoretically; and forming said hybridoma cell by applying a pulsed radiofrequency oscillating field across said electrodes.
Claim 28
Dependent Claim
23 words
The hybridoma cell of claim 23, wherein said antibody producing cells are hyperimmunized mouse spleen cells and the cancer cells are myeloma cells.
References Cited (Backward Citations)
US4452747A
Cited
US4578167A
Cited
US4441972A
Cited
US4476004A
Cited
US4524079A
Cited
US4661451A
Cited
US4665898A
Cited
US4561961A
Cited
US4578168A
Cited
WO1986002377A1
Cited
US4623302A
Cited
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