US4820399A
Expired / Lapsed
US
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Enzyme electrodes
Assignee: Shimadzu Corp
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Filing: 1985-08-28
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Granted: 1989-04-11
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Expires: 2005-08-28
Abstract
Abstract An enzyme electrode composed of a carrier, an enzyme immobilized on a parr of the surface of the carrier, a coating film consisting of a thin film coating the portion where the enzyme is immobilized and being permeable for the substrate for the enzyme and an internal electrode capable of applying voltage to the surface where the enzyme is immobilized, said carrier being impregnated with a substance which acts as an electron transfer mediator, which is useful for measurement of concentration of the substrate in vital samples such as blood serum, blood plasma, urine etc.
Inventors
- 👤 Mitsugi Senda
- 👤 Tokuji Ikeda
- 👤 Isao Katasho
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 15:55
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
67 words
An enzyme electrode including a carrier, said carrier comprising: (a) a storage layer impregnated with a substance capable of functioning as an electron transfer mediator; (b) an enzyme-immobilized layer, positioned adjacent to said storage layer, containing an immobilized enzyme; (c) a thin film coating said enzyme-immobilized layer permeable to a substrate for said enzyme; and (d) an internal electrode capable of applying voltage to said enzyme-immobilized layer.
Claim 2
Dependent Claim
67 words
An enzyme electrode including a carrier, said carrier comprising: (a) a storage layer impregnated with a substance capable of functioning as an electron transfer mediator; (b) an enzyme-immobilized layer, positioned adjacent to said storage layer, containing an immobilized enzyme; (c) a thin film coating said enzyme-immobilized layer permeable to a substrate for said enzyme; and (d) an internal electrode capable of applying voltage to said enzyme-immobilized layer.
Claim 3
Dependent Claim
26 words
The enzyme electrode as defined by claim 1, wherein said storage layer comprises a material selected from the group consisting of porous materials, pastes, and gels.
Claim 4
Dependent Claim
15 words
The enzyme electrode as defined by claim 2, wherein said material is at least semi-conductive.
Claim 5
Dependent Claim
30 words
The enzyme electrode as defined by claim 3, wherein said at least semi-conductive material is selected from the group consisting of graphite, noble metals, high molecular conductors, and inorganic semiconductors.
Claim 6
Dependent Claim
37 words
The enzyme electrode as defined by claim 1, wherein said substance capable of functioning as an electron transfer mediator is selected from the group consisting of p-benzoquinones, ubiquinones, potassium ferricyanide, dichlorophenolindophenol, phenazine methosulfate, and nicotinamide adenine dinucleotide.
Claim 7
Dependent Claim
47 words
The enzyme electrode as defined by claim 1, wherein said storage layer comprises a paste, said substance capable of functioning as an electron transfer mediator is p-benzoquinone, and said p-benzoquinone is present in said paste in an amount of between about 0.2 and 30 percent by weight.
Claim 8
Dependent Claim
51 words
The enzyme electrode as defined by claim 1, wherein said storage layer comprises a paste, said substance capable of functioning as an electron transfer mediator is nicotinamide adenine dinucleotide, and said nicotinamide adenine dinucleotide is present in said paste in an amount of between about 1 and 10 percent by weight.
Claim 9
Dependent Claim
22 words
The enzyme electrode as defined by claim 1, wherein said immobilized enzyme is selected from the group consisting of oxidases and dehydrogenases.
Claim 10
Dependent Claim
31 words
The enzyme electrode as defined by claim 1, wherein said thin film is selected from the group consisting of cellulose acetate film, nitrocellulose film, K-carrageenan gel film, and polyacrylamide gel film.
Claim 11
Dependent Claim
24 words
The enzyme electrode as defined by claim 1, wherein said internal electrode is selected from the group consisting of filamentary electrodes and grid electrodes.
Claim 12
Dependent Claim
36 words
The enzyme electrode as defined by claim 1, wherein said immobilized enzyme is an oxidase selected from the group consisting of glucose oxidase, galactose oxidase, alcohol oxidase, cholesterol oxidase, amino acid oxidase, and uric acid oxidase.
Claim 13
Dependent Claim
33 words
The enzyme electrode as defined by claim 1, wherein said immobilized enzyme is an dehydrogenase selected from the group consisting of glucose dehydrogenase, glutamate dehydrogenase, alcohol dehydrogenase, lactic acid dehydrogenase, and glycerol dehydrogenase.
Claim 14
Dependent Claim
28 words
The enzyme electrode as defined by claim 1, wherein a high enzyme reactivity of said immobilized enzyme is maintained at almost constant level for at least one week.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-04-10 | 1992-10-10 | 1993-04-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-04-10 | 1996-10-10 | 1997-04-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-04-10 | 2000-10-10 | 2001-04-11 | $7700.00 | Window Closed / Lapsed |