US4818690A
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Method for the determination of plasminogen activators (PA)
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1986-04-09
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Granted: 1989-04-04
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Expires: 2006-04-09
Abstract
Abstract A method for the determination of urokinase (UK) or tissue plasminogen activator (t-PA) is described, in which the activator is incubated with plasminogen, with a polysulfuric ester of a saccharide or with a sulfated sugar and, where appropriate, fibrin or fibrin degradation products, and the conversion of plasminogen into plasmin is determined, and agents suitable for this method are described.
Inventors
- 👤 Eric P. Paques
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 10:10
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/56; C12Q2337/00; C12Q2337/10; C12Q2337/12; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/968; G01N2333/972; G01N2333/9723; G01N2333/9726; G01N2400/00; G01N2400/10; G01N2400/38; G01N2400/40
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
68 words
A method for the quantitative determination of a plasminogen activator (PA) from the group consisting of urokinase (UK) and tissue plasminogen activator (tPA) in body fluids, which comprises the steps of incubating PA with plasminogen and with a polysulfuric ester of a saccharide or with a sulfated sugar under conditions effective to allow conversion of the plasminogen into plasmin; and determining quantitatively the conversion of plasminogen into plasmin.
Claim 2
Independent Claim
68 words
A method for the quantitative determination of a plasminogen activator (PA) from the group consisting of urokinase (UK) and tissue plasminogen activator (tPA) in body fluids, which comprises the steps of incubating PA with plasminogen and with a polysulfuric ester of a saccharide or with a sulfated sugar under conditions effective to allow conversion of the plasminogen into plasmin; and determining quantitatively the conversion of plasminogen into plasmin.
Claim 3
Dependent Claim
21 words
The method of claim 1, wherein said incubating step is further conducted in the presence of fibrin or fibrin degredation products.
Claim 4
Dependent Claim
20 words
The method of claim 2, wherein the determining step is further conducted with the use of a chromogenic substrate indicator.
Claim 5
Dependent Claim
25 words
The method as claimed in claim 3, wherein cell membrane constituents are used as the polysulfuric ester of a saccharide or as the sulfated sugar.
Claim 6
Dependent Claim
13 words
The method as claimed in claim 3, wherein the plasminogen activator is t-PA.
Claim 7
Dependent Claim
16 words
The method as claimed in claim 3, wherein the polysulfuric ester of a saccharide is heparin.
Claim 8
Dependent Claim
23 words
The method as claimed in claim 3, wherein heparin is used in a concentration of 10 -8 to 1 mg/ml of assay mixture.
Claim 9
Dependent Claim
45 words
The method as claimed in claim 3, wherein the polysulfuric ester of a saccharide or the sulfated sugar is heparan sulfate, dermatan sulfate, chondroitin sulfate, pentosan sulfate, keratan sulfate or mucopolysaccharide polyslfate, in a concentration of 10 -8 to 1 mg/ml in the assay mixture.
Claim 10
Dependent Claim
30 words
An agent for the quantitative determination of a plasminogen activator comprising at least from about 0.01 to about 1 CTA/ml plasminogen and from about 10 -4 to 1 g/ml heparin.
Claim 11
Dependent Claim
30 words
An agent for the quantitative determination of a plasminogen activator comprising at least from about 0.01 to about 1 CTA/ml plasminogen and from about 10 -4 to 1 g/ml heparin.
Claim 12
Dependent Claim
18 words
The agent of claim 9 further comprising from about 0.01 to about 0.1 ml/100 ml polyoxyethylene sorbitan monooleate.
Claim 13
Dependent Claim
10 words
The agent of claim 10 further comprising fibrin degradation products.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-04-03 | 1992-10-03 | 1993-04-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-04-03 | 1996-10-03 | 1997-04-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-04-03 | 2000-10-03 | 2001-04-04 | $7700.00 | Window Closed / Lapsed |