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US4818685A
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Method and kit for diagnosing bloom's syndrome
Assignee: Temple University
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Filing: 1987-02-12
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Granted: 1989-04-04
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Expires: 2007-02-12
Abstract
Abstract A method and kit for diagnosing Bloom's syndrome are provided relying on specific monoclonal antibodies, such as ATCC HB-9311, which recognize the base excision repair pathway enzyme uracil DNA glycosylase from normal healthy individuals and individuals afflicted with genetic syndromes, but fails to recognize uracil DNA glycosylase from individuals afflicted with Bloom's Syndrome.
Inventors
- 👤 Michael A. Sirover
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 05:57
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/40; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S436/00; Y10S436/811
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
38 words
A method for diagnosing Bloom's syndrome comprising: contacting a human UDG-containing sample with a first anti-human UDG monoclonal antibody which does not recognize Bloom's syndrome UDG; and assaying said sample for binding of UDG by said monoclonal antibody.
Claim 2
Independent Claim
38 words
A method for diagnosing Bloom's syndrome comprising: contacting a human UDG-containing sample with a first anti-human UDG monoclonal antibody which does not recognize Bloom's syndrome UDG; and assaying said sample for binding of UDG by said monoclonal antibody.
Claim 3
Dependent Claim
47 words
A method according to claim 1 including the further step of contacting said sample with a second anti-human UDG monoclonal antibody which recognizes both normal UDG and Bloom's syndrome UDG as a control, and assaying said sample for the binding of UDG by said second monoclonal antibody.
Claim 4
Dependent Claim
42 words
A method of according to claim 2 comprising the further control step of contacting said first monoclonal antibody with normal UDG and assaying for binding of said normal UDG by said antibody to ensure the functional integrity of said first monoclonal antibody.
Claim 5
Dependent Claim
13 words
A method according to claim 1 wherein assaying comprises an enzyme-linked immunosorbent assay.
Claim 6
Dependent Claim
15 words
A method according to claim 4 wherein the monoclonal antibody is a mouse monoclonal antibody.
Claim 7
Dependent Claim
26 words
A method according to claim 5 wherein assaying binding of UDG by said first monoclonal antibody is by means of a detectably labelled anti-mouse Ig antibody.
Claim 8
Dependent Claim
20 words
A method according to claim 6 wherein alkaline phosphatase is conjugated to said anti-mouse Ig anti-body as the detectable label.
Claim 9
Independent Claim
19 words
A method according to any of claims 1, 2, 4 and 6 wherein the monoclonal antibody is ATCC HB-9311.
Claim 10
Dependent Claim
23 words
A method according to claim 2 wherein said second monoclonal antibody is selected from the group consisting of ATCC HB-9312 and ATCC HB-9313.
Claim 11
Dependent Claim
60 words
A kit for the diagnosis of Bloom's syndrome comprising: a carrier for receiving one or more container means; a first container means comprising a first detectably-labelled anti-human UDG monoclonal antibody which does not recognize Bloom's syndrome UDG; and a second container means comprising as a control a detectably-labelled anti-human monoclonal antibody which recognizes both normal UDG and Bloom's syndrome UDG.
Claim 12
Dependent Claim
60 words
A kit for the diagnosis of Bloom's syndrome comprising: a carrier for receiving one or more container means; a first container means comprising a first detectably-labelled anti-human UDG monoclonal antibody which does not recognize Bloom's syndrome UDG; and a second container means comprising as a control a detectably-labelled anti-human monoclonal antibody which recognizes both normal UDG and Bloom's syndrome UDG.
Claim 13
Dependent Claim
81 words
A kit for the diagnosis of Bloom's syndrome comprising: a carrier for receiving one or more container means; a first container means comprising a first anti-human UDG monoclonal antibody which does not recognize Bloom's syndrome UDG; a second container means comprising as a control a second anti-human UDG monoclonal antibody which recognizes both normal UDG and Bloom's syndrome UDG; and a third container means comprising a detectably-labelled secondary antibody or F(ab) fragment capable of binding the first or second monoclonal antibodies.
Claim 14
Dependent Claim
81 words
A kit for the diagnosis of Bloom's syndrome comprising: a carrier for receiving one or more container means; a first container means comprising a first anti-human UDG monoclonal antibody which does not recognize Bloom's syndrome UDG; a second container means comprising as a control a second anti-human UDG monoclonal antibody which recognizes both normal UDG and Bloom's syndrome UDG; and a third container means comprising a detectably-labelled secondary antibody or F(ab) fragment capable of binding the first or second monoclonal antibodies.
Claim 15
Dependent Claim
17 words
A kit according to claim 10 or 11 wherein said antibodies are detectably-labelled with an enzyme label.
Claim 16
Dependent Claim
13 words
A kit according to claim 12 wherein said enzyme label is alkaline phosphatase.
Claim 17
Dependent Claim
21 words
A kit according to claim 10 or 11 wherein the first monoclonal antibody in said first compartment means is ATCC HB-9311.
Claim 18
Dependent Claim
27 words
A kit according to claim 14 wherein the monoclonal antibody in said second compartment means is selected from the group consisting of ATCC HB-9312 and ATCC HB-9313.
Claim 19
Dependent Claim
17 words
A kit according to claim 10 or 11 including an additional container means comprising purified normal UDG.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-04-03 | 1992-10-03 | 1993-04-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-04-03 | 1996-10-03 | 1997-04-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-04-03 | 2000-10-03 | 2001-04-04 | $7700.00 | Window Closed / Lapsed |