Patent US4818527A successfully refreshed from Google Patents.
US4818527A
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US
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T cell epitopes of the hepatitis B virus nucleocapsid protein
Assignee: Scripps Research Institute
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Filing: 1986-12-09
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Granted: 1989-04-04
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Expires: 2006-12-09
Abstract
Abstract Polypeptides corresponding in amino acid residue sequence to T cell stimulating regions of the HBV nucleocapsid protein are disclosed. A method of enhancing the immunogenicity of a polypeptide immunogen comprising operatively linking the polypeptide through an amino acid residue side chain to core protein particles is also disclosed.
Inventors
- 👤 George B. Thornton
- 👤 Ann M. Moriarty
- 👤 David R. Milich
- 👤 Alan McLachlan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 03:10
CPC Classifications
C; C07; C07K; C07K7/00; C07K7/04; C07K7/06; C07K14/00; C07K14/005; A; A61; A61K; A61K39/00; C12; C12N; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122; Y; Y10; Y10S; Y10S530/00; Y10S530/806; Y10S530/807; Y10S930/00; Y10S930/01; Y10S930/22; Y10S930/223
Claims Analysis
Total Claims: 39
Claim 1
Independent Claim
19 words
An immunogenic polypeptide conjugate comprising HBcAg operatively linked through an amino acid residue side chain to a polypeptide immunogen.
Claim 2
Dependent Claim
19 words
An immunogenic polypeptide conjugate comprising HBcAg operatively linked through an amino acid residue side chain to a polypeptide immunogen.
Claim 3
Dependent Claim
17 words
The polypeptide conjugate of claim 1 wherein said HBcAg is present as core protein in particulate form.
Claim 4
Dependent Claim
41 words
The polypeptide conjugate of claim 1 wherein said polypeptide immunogen is a pathogen related immunogen, and said conjugate having the capacity of inducing the production of antibodies that immunoreact with said pathogen when injected in an effective amount into an animal.
Claim 5
Dependent Claim
23 words
The polypeptide conjugate of claim 1 wherein said polypeptide immunogen is a pathogen related immunogen that immunoreacts with antibodies induced by said pathogen.
Claim 6
Dependent Claim
17 words
The polypeptide conjugate of claim 3 wherein said pathogen is HBV and said polypeptide immunogen is HBsAg.
Claim 7
Dependent Claim
18 words
An immunogenic fusion protein comprising HBcAg protein operatively linked by a peptide bond to a pathogen related immunogen.
Claim 8
Dependent Claim
18 words
An immunogenic fusion protein comprising HBcAg protein operatively linked by a peptide bond to a pathogen related immunogen.
Claim 9
Dependent Claim
18 words
The fusion protein of claim 6 wherein said pathogen related immunogen immunoreacts with antibodies induced by said pathogen.
Claim 10
Dependent Claim
23 words
The fusion protein of claim 6 wherein said pathogen related immunogen is operatively linked to the amino-terminal amino acid residue of said HBcAg.
Claim 11
Dependent Claim
23 words
An immunogenic fusion protein comprising HBcAg protein operatively linked at its carboxy-terminus by a peptide bond to the amino-terminus of a polypeptide immunogen.
Claim 12
Dependent Claim
23 words
An immunogenic fusion protein comprising HBcAg protein operatively linked at its carboxy-terminus by a peptide bond to the amino-terminus of a polypeptide immunogen.
Claim 13
Dependent Claim
15 words
The immunogenic fusion protein of claim 9 wherein said polypeptide immunogen is pathogen related immunogen.
Claim 14
Dependent Claim
72 words
A T cell stimulating polypeptide consisting essentially of about 15 to about 70 amino acid residues having a sequence corresponding to a portion of the amino acid residue sequence of HBV core protein from about position 70 to about position 140 from the amino terminus thereof, said polypeptide including the amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: TWVGVNLEDPASRDLVVSYVNTNMG.
Claim 15
Dependent Claim
72 words
A T cell stimulating polypeptide consisting essentially of about 15 to about 70 amino acid residues having a sequence corresponding to a portion of the amino acid residue sequence of HBV core protein from about position 70 to about position 140 from the amino terminus thereof, said polypeptide including the amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: TWVGVNLEDPASRDLVVSYVNTNMG.
Claim 16
Dependent Claim
72 words
A T cell stimulating polypeptide consisting essentially of about 15 to about 70 amino acid residues having a sequence corresponding to a portion of the amino acid residue sequence of HBV core protein from about position 70 to about position 140 from the amino terminus thereof, said polypeptide including the amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: LLWFHISCLTFGRETVIEYLV.
Claim 17
Dependent Claim
72 words
A T cell stimulating polypeptide consisting essentially of about 15 to about 70 amino acid residues having a sequence corresponding to a portion of the amino acid residue sequence of HBV core protein from about position 70 to about position 140 from the amino terminus thereof, said polypeptide including the amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: LLWFHISCLTFGRETVIEYLV.
Claim 18
Dependent Claim
72 words
A T cell stimulating polypeptide consisting essentially of about 15 to about 70 amino acid residues having a sequence corresponding to a portion of the amino acid residue sequence of HBV core protein from about position 70 to about position 140 from the amino terminus thereof, said polypeptide including the amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: VVSYVNTNMGLKFRQL.
Claim 19
Dependent Claim
72 words
A T cell stimulating polypeptide consisting essentially of about 15 to about 70 amino acid residues having a sequence corresponding to a portion of the amino acid residue sequence of HBV core protein from about position 70 to about position 140 from the amino terminus thereof, said polypeptide including the amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: VVSYVNTNMGLKFRQL.
Claim 20
Dependent Claim
72 words
A T cell stimulating polypeptide consisting essentially of about 15 to about 70 amino acid residues having a sequence corresponding to a portion of the amino acid residue sequence of HBV core protein from about position 70 to about position 140 from the amino terminus thereof, said polypeptide including the amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: VSFGVWIRTPPAYRPPNAPIL.
Claim 21
Dependent Claim
72 words
A T cell stimulating polypeptide consisting essentially of about 15 to about 70 amino acid residues having a sequence corresponding to a portion of the amino acid residue sequence of HBV core protein from about position 70 to about position 140 from the amino terminus thereof, said polypeptide including the amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: VSFGVWIRTPPAYRPPNAPIL.
Claim 22
Dependent Claim
33 words
A T cell stimulating polypeptide wherein the amino acid residue sequence corresponds to the sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: TWVGVNLEDPASRDLVVSYVNTNMG.
Claim 23
Dependent Claim
33 words
A T cell stimulating polypeptide wherein the amino acid residue sequence corresponds to the sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: TWVGVNLEDPASRDLVVSYVNTNMG.
Claim 24
Dependent Claim
33 words
A T cell stimulating polypeptide wherein the amino acid residue sequence corresponds to the sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: LLWFHISCLTFGRETVIEYLV.
Claim 25
Dependent Claim
33 words
A T cell stimulating polypeptide wherein the amino acid residue sequence corresponds to the sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: LLWFHISCLTFGRETVIEYLV.
Claim 26
Dependent Claim
33 words
A T cell stimulating polypeptide wherein the amino acid residue sequence corresponds to the sequence taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: VVSYVNTNMGLKFRQL.
Claim 27
Dependent Claim
33 words
A T cell stimulating polypeptide wherein the amino acid residue sequence corresponds to the sequence taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: VVSYVNTNMGLKFRQL.
Claim 28
Dependent Claim
33 words
A T cell stimulating polypeptide wherein the amino acid residue sequence corresponds to the sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: VSFGVWIRTPPAYRPPNAPIL.
Claim 29
Dependent Claim
33 words
A T cell stimulating polypeptide wherein the amino acid residue sequence corresponds to the sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: VSFGVWIRTPPAYRPPNAPIL.
Claim 30
Dependent Claim
66 words
A composite polypeptide immunogen comprising a T cell stimulating polypeptide of no more than 70 residues operatively linked through an amino acid residue side chain to a polypeptide immunogen, said T cell stimulating polypeptide having an amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: LLWFHISCLTFGRETVIEYLV; and wherein said polypeptide immunogen is particulate HBsAg.
Claim 31
Dependent Claim
66 words
A composite polypeptide immunogen comprising a T cell stimulating polypeptide of no more than 70 residues operatively linked through an amino acid residue side chain to a polypeptide immunogen, said T cell stimulating polypeptide having an amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: LLWFHISCLTFGRETVIEYLV; and wherein said polypeptide immunogen is particulate HBsAg.
Claim 32
Dependent Claim
88 words
A composite polypeptide immunogen comprising a T cell stimulating polypeptide of no more than 70 residues operatively linked by a peptide bond to a polypeptide immunogen, said T cell stimulating polypeptide having an amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: LLWFHISCLTFGRETVIEYLV; and said polypeptide immunogen consists essentially of a polypeptide having an amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: DPRVRGLYFPAGG.
Claim 33
Dependent Claim
88 words
A composite polypeptide immunogen comprising a T cell stimulating polypeptide of no more than 70 residues operatively linked by a peptide bond to a polypeptide immunogen, said T cell stimulating polypeptide having an amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: LLWFHISCLTFGRETVIEYLV; and said polypeptide immunogen consists essentially of a polypeptide having an amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: DPRVRGLYFPAGG.
Claim 34
Dependent Claim
88 words
A composite polypeptide immunogen comprising a T cell stimulating polypeptide of no more than 70 residues operatively linked by a peptide bond to a polypeptide immunogen, said T cell stimulating polypeptide having an amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: VSFGVWIRTPPAYRPPNAPIL; and said polypeptide immunogen consists essentially of a polypeptide having an amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: DPRVRGLY.
Claim 35
Dependent Claim
88 words
A composite polypeptide immunogen comprising a T cell stimulating polypeptide of no more than 70 residues operatively linked by a peptide bond to a polypeptide immunogen, said T cell stimulating polypeptide having an amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: VSFGVWIRTPPAYRPPNAPIL; and said polypeptide immunogen consists essentially of a polypeptide having an amino acid residue sequence, taken from left to right and in the direction of amino-terminus to carboxy-terminus, represented by the formula: DPRVRGLY.
Claim 36
Independent Claim
24 words
A method of enhancing the immunogenicity of a polypeptide immunogen comprising operatively linking said immunogen to HBcAg through an amino acid residue side chain.
Claim 37
Independent Claim
24 words
A method of enhancing the immunogenicity of a polypeptide immunogen comprising operatively linking said immunogen to HBcAg through an amino acid residue side chain.
Claim 38
Independent Claim
22 words
A method of enhancing the immunogenicity of a pathogen related immunogen comprising operatively linking said immunogen to HBcAg by a peptide bond.
Claim 39
Independent Claim
22 words
A method of enhancing the immunogenicity of a pathogen related immunogen comprising operatively linking said immunogen to HBcAg by a peptide bond.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-04-03 | 1992-10-03 | 1993-04-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-04-03 | 1996-10-03 | 1997-04-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-04-03 | 2000-10-03 | 2001-04-04 | $7700.00 | Window Closed / Lapsed |