Patent US4812404A successfully refreshed from Google Patents.
US4812404A
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Apatite immobilized glucanase
Assignee: Dental Kagaku KK
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Filing: 1986-06-25
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Granted: 1989-03-14
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Expires: 2006-06-25
Abstract
Abstract Apatite containing immobilized glucanase for decomposition of polysaccharies associated with dental carries is prepared by adding dropwise glutaraldehyde to an aqueous solution in which glucanase, protein and apatite are present in a mixed state. The protein in preferably lysozyme.
Inventors
- 👤 Yoshinori Kuboki
- 👤 Daisaburo Fujimoto
- 👤 Hideki Aoki
- 👤 Keijiro Fujita
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 21:25
CPC Classifications
A; A61; A61K; A61K8/00; A61K8/18; A61K8/19; A61K8/24; A61K8/30; A61K8/64; A61K8/66; A61Q; A61Q11/00; C; C12; C12N; C12N11/00; C12N11/14; C12N11/18; A61K2800/00; A61K2800/40; A61K2800/56
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
38 words
A method for preparing an apatite having glucanase immobilized therein, comprising adding dropwise glutaraldehyde to an aqueous solution in which glucanase, lysozyme and apatite are present in a mixed state and recovering an apatite having glucanase immobilized therein.
Claim 2
Independent Claim
38 words
A method for preparing an apatite having glucanase immobilized therein, comprising adding dropwise glutaraldehyde to an aqueous solution in which glucanase, lysozyme and apatite are present in a mixed state and recovering an apatite having glucanase immobilized therein.
Claim 3
Dependent Claim
24 words
The method according to claim 1, wherein the aqueous solution used is a buffer solution having a pH value ranging from 5.6 to 9.0.
Claim 4
Dependent Claim
21 words
The method according to claim 1, wherein the amount of glutaraldehyde is 0.3 to 6% relative to the amount of lysozyme
Claim 5
Dependent Claim
18 words
The method according to claim 1, wherein the apatite has a particle size of 2 to 200 microns.
Claim 6
Dependent Claim
10 words
The method of claim 1, wherein the glucanase is levanase.
Claim 7
Dependent Claim
10 words
The method of claim 1, wherein the glucanase is dextranase.
Claim 8
Dependent Claim
10 words
The method of claim 1, wherein the glucanase is mutanase.
Claim 9
Dependent Claim
10 words
The method of claim 1, wherein the apatite is hydroxyapatite.
Claim 10
Dependent Claim
10 words
The method of claim 1, wherein the apatite is fluro-apatite.
Claim 11
Independent Claim
85 words
A method of preparing an apatite with glucanase immobilized therein, comprising mixing a buffered aqueous solution having a pH ranging from 5.6 to 9.0 containing glucanase and lysozyme in equivalent amounts and apatite, said apatite having a particle size of 2 to 200 microns in an amount of 10 to 100 times as large as the amount of lysozyme, while adding glutaraldehyde dropwise thereto in an amount of 0.3% to 6% relative to the amount of lysozyme, and recovering an apatite having glucanase immobilized therein.
Claim 12
Independent Claim
85 words
A method of preparing an apatite with glucanase immobilized therein, comprising mixing a buffered aqueous solution having a pH ranging from 5.6 to 9.0 containing glucanase and lysozyme in equivalent amounts and apatite, said apatite having a particle size of 2 to 200 microns in an amount of 10 to 100 times as large as the amount of lysozyme, while adding glutaraldehyde dropwise thereto in an amount of 0.3% to 6% relative to the amount of lysozyme, and recovering an apatite having glucanase immobilized therein.
References Cited (Backward Citations)
US4464468A
Cited
US4155546A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-03-13 | 1992-09-12 | 1993-03-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-03-13 | 1996-09-12 | 1997-03-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-03-13 | 2000-09-12 | 2001-03-14 | $7700.00 | Window Closed / Lapsed |