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US4812220A
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Enzyme sensor for determining a concentration of glutamate
Assignee: Unitika Ltd
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Filing: 1987-08-12
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Granted: 1989-03-14
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Expires: 2007-08-12
Abstract
Abstract Disclosed is an enzyme sensor for determining a concentration of glutamate comprising an immobilize enzyme acting specifically to a substrate and a transducer for converting the quantitative change of a substance or heat which is produced or consumed during an enzyme reaction to an electrical signal, wherein the enzyme is glutamine synthetase and the transducer is the pH glass electrode or ion-sensitive field-effect transistor (ISFET). The enzyme sensor can be miniaturized and can accurately determine a concentration of glutamate even when it is low.
Inventors
- 👤 Takeaki Iida
- 👤 Takeshi Kawabe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 14:36
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; G; G01; G01N; G01N27/00; G01N27/26; G01N27/403; G01N27/414; G01N27/4145; G01N33/00; G01N33/02; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
62 words
An enzyme sensor for determining a concentration of glutamate comprising an immobilize enzyme acting specifically to a substrate and a transducer for converting the quantitative change of a substance or heat which is produced or consumed during an enzyme reaction to an electrical signal, wherein the enzyme is glutamine synthetase and the transducer is the pH glass electrode or ion-sensitive field-effect transistor.
Claim 2
Dependent Claim
62 words
An enzyme sensor for determining a concentration of glutamate comprising an immobilize enzyme acting specifically to a substrate and a transducer for converting the quantitative change of a substance or heat which is produced or consumed during an enzyme reaction to an electrical signal, wherein the enzyme is glutamine synthetase and the transducer is the pH glass electrode or ion-sensitive field-effect transistor.
Claim 3
Dependent Claim
26 words
The enzyme sensor according to claim 1 wherein glutamine synthetase is isolated from a microorganism having a most suitable growth temperature of 50° to 85° C.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-03-13 | 1992-09-12 | 1993-03-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-03-13 | 1996-09-12 | 1997-03-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-03-13 | 2000-09-12 | 2001-03-14 | $7700.00 | Window Closed / Lapsed |