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US4806474A
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US
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Preparation of mycelial chitosan and glucan fractions from microbial biomass
Assignee: Haarmann and Reimer Corp
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Filing: 1985-06-10
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Granted: 1989-02-21
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Expires: 2005-06-10
Abstract
Abstract Chitin from microbial biomass is converted to chitosan and recovered as an essentially glucan-free product. The method involves contacting the biomass with a strong alkali solution at a temperature within the range of from 60 DEG to 90 DEG C. for a period of at least 10 hours to form an insoluble chitosan/glucan residue. The chitosan is then recovered by adding dilute acid to the residue to dissolve it without dissolving the glucan which can then be recovered separately.
Inventors
- 👤 Donald F. Hershberger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 22:23
CPC Classifications
C; C08; C08B; C08B37/00; C08B37/0006; C08B37/0024; C08B37/0027; C08B37/003; C12; C12P; C12P19/00; C12P19/26; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/913; Y10S435/917
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
115 words
A method for recovering chitosan from chitin containing biomass which comprises the steps of: (a) contacting damp biomass with a concentrated aqueous solution of a strong alkali wherein the amount of alkali is from 200% to 500% of the amount of biomass on a dry weight basis; (b) maintaining the biomass in contact with the alkali solution for a period of at least 10 hours while maintaining the mixture's temperature in the range of from 60° to 90° C. to convert the chitin in the biomass to chitosan; (c) separating the chitosan from the alkali solution and washing it; and (d) recovering the chitosan from undissolved residue from step c by dissolution in dilute acid.
Claim 2
Independent Claim
115 words
A method for recovering chitosan from chitin containing biomass which comprises the steps of: (a) contacting damp biomass with a concentrated aqueous solution of a strong alkali wherein the amount of alkali is from 200% to 500% of the amount of biomass on a dry weight basis; (b) maintaining the biomass in contact with the alkali solution for a period of at least 10 hours while maintaining the mixture's temperature in the range of from 60° to 90° C. to convert the chitin in the biomass to chitosan; (c) separating the chitosan from the alkali solution and washing it; and (d) recovering the chitosan from undissolved residue from step c by dissolution in dilute acid.
Claim 3
Dependent Claim
13 words
The method of claim 1 wherein the alkali is NaOH, LiOH or KOH.
Claim 4
Dependent Claim
10 words
The method of claim 2 wherein the alkali is NaOH.
Claim 5
Dependent Claim
24 words
The method of claim 1 wherein the alkali is at least 40% in terms of its content in the aqueous phase of the solution.
Claim 6
Dependent Claim
34 words
The method of claim 1 wherein the biomass is maintained in contact with the alkali solution for a period of from 16 to 24 hours at a temperature of from 75° to 85° C.
Claim 7
Dependent Claim
41 words
The method of claim 1 wherein the residue is placed in dilute acid solution by the addition of acetic, formic, citric or hydrochloric acid in sufficient quantity to lower the pH to a level within the range of 3 to 5.
Claim 8
Dependent Claim
21 words
The method of claim 1 wherein the biomass is recovered from the fermentation of an organism from the species Aspergillus niger.
Claim 9
Dependent Claim
33 words
The method of claim 1 wherein the chitosan is recovered from the dilute acid solution thereof formed in step d by adding base to the solution in sufficient amount to precipitate the chitosan.
Claim 10
Independent Claim
216 words
A method for the recovery of chitosan from chitin containing biomass produced by the deep fermentation of an organism of the species Aspergillus niger which fermentation process provides damp biomass upon filtration and which method comprises the steps of: (a) contacting the damp biomass with an aqueous solution of NaOH containing at least 40 weight percent NaOH in terms of its content in the aqueous phase of the solution wherein sufficient solution is used to provide an amount of NaOH of from 200% to 500% of the amount of biomass on a dry weight basis; (b) maintaining the biomass in contact with the NaOH solution for a period of time of from 16 to 24 hours while maintaining the temperature in the range of from 7520 C. to 85° C. to convert the chitin in the biomass to chitosan; (c) separating the chitosan from the NaOH solution by repeated slurrying in water followed by centrifugation and decantation to recover a washed undissolved residue containing chitosan and glucan; (d) recovering the chitosan from the washed residue by the addition thereto of sufficient acid to lower the pH to a lever of from 3 to 5 to solubilize the chitosan without solubilizing the glucan; and (e) separating the solubilized chitosan from the insoluble glucan by liquid/solid separatory techniques.
Claim 11
Independent Claim
216 words
A method for the recovery of chitosan from chitin containing biomass produced by the deep fermentation of an organism of the species Aspergillus niger which fermentation process provides damp biomass upon filtration and which method comprises the steps of: (a) contacting the damp biomass with an aqueous solution of NaOH containing at least 40 weight percent NaOH in terms of its content in the aqueous phase of the solution wherein sufficient solution is used to provide an amount of NaOH of from 200% to 500% of the amount of biomass on a dry weight basis; (b) maintaining the biomass in contact with the NaOH solution for a period of time of from 16 to 24 hours while maintaining the temperature in the range of from 7520 C. to 85° C. to convert the chitin in the biomass to chitosan; (c) separating the chitosan from the NaOH solution by repeated slurrying in water followed by centrifugation and decantation to recover a washed undissolved residue containing chitosan and glucan; (d) recovering the chitosan from the washed residue by the addition thereto of sufficient acid to lower the pH to a lever of from 3 to 5 to solubilize the chitosan without solubilizing the glucan; and (e) separating the solubilized chitosan from the insoluble glucan by liquid/solid separatory techniques.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-02-21 | 1992-08-22 | 1993-02-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-02-21 | 1996-08-22 | 1997-02-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-02-21 | 2000-08-22 | 2001-02-21 | $7700.00 | Window Closed / Lapsed |