Patent US4806415A successfully refreshed from Google Patents.
US4806415A
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Method and system for determining the presence of adenosine triphosphate or flavin mononucleotide
Assignee: Bayer Corp
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Filing: 1984-09-13
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Granted: 1989-02-21
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Expires: 2004-09-13
Abstract
Abstract An enzymatic determination of one of the co-enzymes adenosine triphosphate (ATP) or flavin mono-nucleotide (FMN) based on the use of an flavine adenine dinucleotide (FAD) synthetase-active system in conjunction with the other coenzyme to generate FAD. The generated FAD can then be measured to determine the ATP or FMN concentration in the sample. Preferably, the generated FAD is measured by combination with a corresponding apoenzyme, preferably apo (glucose oxidase), and measuring the activity of the enzyme produced. The resultant active enzyme is preferably measured in a manner which results in a colorimetric response. The present invention provides an ATP or FMN assay which combines the sensitivity of bioluminescent assays with the convenience of a colorimetrically detectable response.
Inventors
- 👤 Piero Fossati
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 03:05
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48; C12Q1/008; C12Q1/02; C12Q1/04
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
72 words
A reagent system for the determination of a coenzyme selected from the group consisting adenosine triphosphate and flavin mononucleotide in an aqueous liquid sample, comprising: (a) a coenzyme selected from adenine flavin mononucleotide triphosphate and flavin mononucleotide, one of which is the substance to be determined and the other of which is a component of the reagent system; (b) an enzyme preparation having flavin adenine dinucleotide synthetase activity; and (c) apo(glucose oxidase).
Claim 2
Dependent Claim
72 words
A reagent system for the determination of a coenzyme selected from the group consisting adenosine triphosphate and flavin mononucleotide in an aqueous liquid sample, comprising: (a) a coenzyme selected from adenine flavin mononucleotide triphosphate and flavin mononucleotide, one of which is the substance to be determined and the other of which is a component of the reagent system; (b) an enzyme preparation having flavin adenine dinucleotide synthetase activity; and (c) apo(glucose oxidase).
Claim 3
Dependent Claim
29 words
The reagent system of claim 1, additionally comprising glucose, peroxidase and a chromogenic indicator capable of providing a detectable colorimetric response in the presence of hydrogen peroxide and peroxidase.
Claim 4
Dependent Claim
95 words
An analytical method for determining adenosine triphosphate ("ATP") in picomole concentrations, said method comprising the steps of: combining a liquid sample with a reagent system, said system comprising in a single solution: flavin mononucleotide ("FMN"); flavin adenine dinucleotide ("FAD") synthetase; apo(glucose oxidase); a peroxidatively active substance; glucose; a metal cation; and a photogen, whereby said photogen provides a detectable optical response in the presence of hydrogen peroxide and the peroxidatively active substance, and detecting the optical response said response being an indication of the amount of ATP down to picomole concentrations in said liquid sample.
Claim 5
Dependent Claim
95 words
An analytical method for determining adenosine triphosphate ("ATP") in picomole concentrations, said method comprising the steps of: combining a liquid sample with a reagent system, said system comprising in a single solution: flavin mononucleotide ("FMN"); flavin adenine dinucleotide ("FAD") synthetase; apo(glucose oxidase); a peroxidatively active substance; glucose; a metal cation; and a photogen, whereby said photogen provides a detectable optical response in the presence of hydrogen peroxide and the peroxidatively active substance, and detecting the optical response said response being an indication of the amount of ATP down to picomole concentrations in said liquid sample.
Claim 6
Dependent Claim
14 words
The analytical reagent system of claim 3 wherein the metal cation comprises magnesium ion.
Claim 7
Dependent Claim
32 words
A reagent test strip for determining adenosine triphosphate ("ATP") in concentrations as low as a picomole per liter, said strip having a carrier matrix comprising the analytical reagent system of claim 4.
Claim 8
Dependent Claim
30 words
An analytical assay method comprising the method of claim 3 whereby the determination of ATP is used to assay biological activities or substances which relate to the existence of ATP.
Claim 9
Dependent Claim
55 words
The analytical assay method of claim 6, whereby said method is used to assay creatin kinase, cyclic adenosine monophosphate, creatine phosphate, glucose, guanosine triphosphate, triglycerides, ATP-sulphurylase, ATP-ase, myokinase, pyruvate kinase, 5'-nucleotidase, hexokinase, guanosine monophosphate phosphodiesterase, protein binding reactions relating to the existence of ATP, sperm viability, erythrocyte viability, platelet viability, or tissue or cell viability.
Claim 10
Dependent Claim
96 words
An analytical method for determining flavin mononucleotide ("FMN") in picomole concentrations, said method comprising the steps of: combining a liquid sample with a reagent system, said system comprising in a single solution: adenosine triphosphate ("ATP"); flavin adenine dinucleotide ("FAD") synthetase; apo(glucose oxidase); a peroxidatively active substance; glucose; a metal cation; and a photogen, whereby said photogen provides a detectable optical response in the presence of a hydrogen peroxide and the peroxidatively active substance, and detecting the optical response said response being an indication of the amount of FMN down to picomole concentrations in said liquid sample.
Claim 11
Dependent Claim
96 words
An analytical method for determining flavin mononucleotide ("FMN") in picomole concentrations, said method comprising the steps of: combining a liquid sample with a reagent system, said system comprising in a single solution: adenosine triphosphate ("ATP"); flavin adenine dinucleotide ("FAD") synthetase; apo(glucose oxidase); a peroxidatively active substance; glucose; a metal cation; and a photogen, whereby said photogen provides a detectable optical response in the presence of a hydrogen peroxide and the peroxidatively active substance, and detecting the optical response said response being an indication of the amount of FMN down to picomole concentrations in said liquid sample.
Claim 12
Dependent Claim
14 words
The analytical reagent system of claim 8 wherein the metal cation comprises magnesium ion.
Claim 13
Dependent Claim
32 words
A reagent test strip for determining flavin mononucleotide ("FMN") in concentrations as low as a picomole per liter, said strip having a carrier matrix comprising the analytical reagent system of claim 9.
Claim 14
Dependent Claim
57 words
An analytical reagent system for determining adenosine triphosphate ("ATP") in picomole concentrations, said reagent system comprising: flavin mononucleotide ("FMN"); flavin adenine dinucleotide ("FAD") synthetase; apo(glucose oxidase); a peroxidatively active substance; glucose; a metal cation; and a photogen, whereby said photogen provides a detectable optical response in the presence of a hydrogen peroxide and the peroxidatively active substance.
Claim 15
Dependent Claim
57 words
An analytical reagent system for determining adenosine triphosphate ("ATP") in picomole concentrations, said reagent system comprising: flavin mononucleotide ("FMN"); flavin adenine dinucleotide ("FAD") synthetase; apo(glucose oxidase); a peroxidatively active substance; glucose; a metal cation; and a photogen, whereby said photogen provides a detectable optical response in the presence of a hydrogen peroxide and the peroxidatively active substance.
Claim 16
Dependent Claim
57 words
An analytical reagent system for determining flavin mononucleotide ("FMN") in picomole concentrations, said reagent system comprising: adenosine triphosphate ("ATP"); flavin adenine dinucleotide ("FAD") synthetase; apo(glucose oxidase); a peroxidatively active substance; glucose; a metal cation; and a photogen, whereby said photogen provides a detectable optical response in the presence of a hydrogen peroxide and the peroxidatively active substance.
Claim 17
Dependent Claim
57 words
An analytical reagent system for determining flavin mononucleotide ("FMN") in picomole concentrations, said reagent system comprising: adenosine triphosphate ("ATP"); flavin adenine dinucleotide ("FAD") synthetase; apo(glucose oxidase); a peroxidatively active substance; glucose; a metal cation; and a photogen, whereby said photogen provides a detectable optical response in the presence of a hydrogen peroxide and the peroxidatively active substance.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-02-21 | 1992-08-22 | 1993-02-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-02-21 | 1996-08-22 | 1997-02-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-02-21 | 2000-08-22 | 2001-02-21 | $7700.00 | Window Closed / Lapsed |