US4806352A
Expired / Lapsed
US
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Immunological lipid emulsion adjuvant
Assignee: Ribi Immunochem Research Inc
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Filing: 1986-04-15
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Granted: 1989-02-21
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Expires: 2006-04-15
Abstract
Abstract An immunological adjuvant is provided which enhances the immune response against antigens, and accordingly is useful in vaccines. The adjuvant system is comprised of (1) a lipid emulsion system containing a metabolizable oil, a low molecular weight polyol and lecithin and (2) at least one refined detoxified bacterial biological adjuvant.
Inventors
- 👤 John L. Cantrell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 11:23
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/39; A61K2039/555; A61K2039/55511; A61K2039/55544; A61K2039/5555; A61K2039/55566; A61K2039/55588; A61K2039/55594
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
41 words
An immunological adjuvant useful for enhancing the immune response against antigens, in a host, comprised of: (1) a lipid emulsion system containing, (a) a metabolizable oil, (b) a low molecular weight polyol, (c) lecithin, and (2) a refined, detoxified bacterial adjuvant.
Claim 2
Dependent Claim
41 words
An immunological adjuvant useful for enhancing the immune response against antigens, in a host, comprised of: (1) a lipid emulsion system containing, (a) a metabolizable oil, (b) a low molecular weight polyol, (c) lecithin, and (2) a refined, detoxified bacterial adjuvant.
Claim 3
Dependent Claim
25 words
The adjuvant of claim 1 wherein said metabolizable oil is a fatty oil comprised mainly of the diglycerides and triglycerides of oleic and linoleic acids.
Claim 4
Dependent Claim
21 words
The adjuvant of claim 1 wherein said metabolizable oil is selected from the group consisting of vegetable oils and animal oils.
Claim 5
Dependent Claim
25 words
The adjuvant of claim 3 wherein said vegetable oil is selected from the group consisting of peanut oil, sunflower seed oil and safflower seed oil.
Claim 6
Dependent Claim
11 words
The adjuvant of claim 3 wherein said animal oil is squalene.
Claim 7
Dependent Claim
48 words
The adjuvant of claim 1 wherein said lipid emulsion system is comprised of from about 30 to about 60 percent by weight of metabolizable oil; from about 30 to 60 percent by weight of a polyol, and from about 1 to about 15 percent by weight of lecithin.
Claim 8
Dependent Claim
13 words
An adjuvant system combining the immunological adjuvant of claim 1 with an antigen.
Claim 9
Dependent Claim
22 words
The adjuvant system of claim 7 wherein said antigen and said refined detoxified bacterial adjuvant are contained in a sterile saline solution.
Claim 10
Dependent Claim
43 words
The adjuvant of claim 8 wherein the concentration of antigen in said sterile saline solution is from about 5 to about 5000 μg/ml and the concentration of refined detoxified bacterial adjuvant in said sterile saline solution is from about 125 to 1000 μg/ml.
Claim 11
Dependent Claim
91 words
The adjuvant of claim 7 wherein said lipid emulsion system is comprised of a sterile saline solution of from about 30 to about 60 percent by weight of metabolizable oil; from about 30 to 60 percent by weight of a polyol, and from about 1 to about 15 percent by weight of lecithin, and the concentration of antigen in said sterile saline solution is from about 5 to about 5000 μg/ml and the concentration of refined detoxified bacterial adjuvant in said sterile saline solution is from about 125 to 1000 μg/ml.
Claim 12
Dependent Claim
28 words
The adjuvant of claim 7 wherein said refined detoxified bacterial adjuvant is selected from the group consisting of refined detoxified endotoxin, trehalose dimycolate or protein from Salmonella typhimurium.
Claim 13
Dependent Claim
15 words
The adjuvant of claim 7 wherein said refined detoxified bacterial adjuvant is refined detoxified endotoxin.
Claim 14
Dependent Claim
43 words
A method of enhancing the immune response in a host against an antigen which is capable of eliciting an immune response in said host, which comprises administering to said host an immune response enhancing amount of the immunological adjuvant system of claim 7.
Claim 15
Dependent Claim
43 words
A method of enhancing the immune response in a host against an antigen which is capable of eliciting an immune response in said host, which comprises administering to said host an immune response enhancing amount of the immunological adjuvant system of claim 10.
Claim 16
Dependent Claim
31 words
The method of claim 13 wherein the immunological adjuvant system is administered by a single injection containing from about 25 to about 200 μg of refined detoxified bacterial adjuvant per dose.
Claim 17
Dependent Claim
13 words
The method of claim 13 wherein said antigen is a genetically engineered protein.
Claim 18
Dependent Claim
14 words
The method of claim 13 wherein said antigen is an in vitro synthesized peptide.
Claim 19
Dependent Claim
16 words
The method of claim 13 wherein said antigen is a hepten conjugated with a carrier molecule.
Claim 20
Dependent Claim
13 words
The method of claim 13 wherein said antigen is a naturally occurring protein.
Claim 21
Dependent Claim
18 words
The method of claim 13 wherein said antigen is an inactivated preparation of virus, bacteria, fungi, or protozoa.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-02-21 | 1992-08-22 | 1993-02-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-02-21 | 1996-08-22 | 1997-02-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-02-21 | 2000-08-22 | 2001-02-21 | $7700.00 | Window Closed / Lapsed |