US4036953A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for preparing vaccine adjuvant
Assignee: Bpifrance Financement SA
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Filing: 1972-11-17
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Granted: 1977-07-19
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Expires: 1992-11-17
Abstract
Abstract Water-soluble immunological adjuvants, extracted from mycobacteria and nocardia cells, useful for stimulating, in a host, the immune response to various kinds of antigens.
Inventors
- 👤 Arlette Adam
- 👤 Frank M. Berger
- 👤 Louis Chedid
- 👤 Edgar Lederer
- 👤 Jean-Francois Petit
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 15:27
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/66; A61K35/74; A61K39/00; A61K39/39; C; C07; C07K; C07K14/00; C07K14/195; C07K14/35; A61K2039/555; A61K2039/55511; A61K2039/55566; A61K2039/55588; A61K2039/55594; A61K38/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/863; Y10S435/866; Y10S435/867; Y10S435/869; Y10S435/872
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
82 words
In a process for preparing a water-soluble immunological non-specific adjuvant for enhancing the effect of vaccine, the improvement which comprises treating with a murolytic enzyme, in an aqueous solution, a suspension of Mycobacteria or Nocardia cells walls, said cell walls being substantially free of waxes, free lipids, free proteins and of nucleic acids, separating the solid residue including cell walls residue from the digested residue and, recovering the aqueous portion containing water-soluble immunological adjuvant, the adjuvant having reduced sensitizing action to tuberculin.
Claim 2
Dependent Claim
82 words
In a process for preparing a water-soluble immunological non-specific adjuvant for enhancing the effect of vaccine, the improvement which comprises treating with a murolytic enzyme, in an aqueous solution, a suspension of Mycobacteria or Nocardia cells walls, said cell walls being substantially free of waxes, free lipids, free proteins and of nucleic acids, separating the solid residue including cell walls residue from the digested residue and, recovering the aqueous portion containing water-soluble immunological adjuvant, the adjuvant having reduced sensitizing action to tuberculin.
Claim 3
Dependent Claim
11 words
The process of claim 1 wherein the murolytic enzyme is lysozyme.
Claim 4
Dependent Claim
38 words
The process of claim 2 which comprises the additional step of lyophilizing the aqueous fraction containing the immunological adjuvant and collecting a water-soluble product which is insoluble in ether, chloroform, acetone and a mixture of ethanol and chloroform.
Claim 5
Dependent Claim
20 words
The process of claim 3 which comprises the additional step of making a aqueous solution of the dry lyophilized extract.
Claim 6
Dependent Claim
37 words
The process of claim 4 which comprises the additional step of filtering the aqueous solution of the immunological adjuvant on a molecular sieve and recovering the filtrate containing the more purified aqueous solution of the immunological adjuvant.
Claim 7
Dependent Claim
14 words
The process of claim 5 wherein the molecular sieve is of a polydextran gel.
Claim 8
Dependent Claim
34 words
The process of claim 6 which comprises taking off several fractions from the column and collecting either the first or second fraction of the aqueous filtrate, said fractions containing the more purified immunological adjuvant.
References Cited (Backward Citations)
US3529057A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-07-18 | 1981-01-17 | 1981-07-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-07-18 | 1985-01-17 | 1985-07-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-07-18 | 1989-01-17 | 1989-07-19 | $7700.00 | Window Closed / Lapsed |