Patent US4804537A successfully refreshed from Google Patents.
US4804537A
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Sperm selection process using a salt of hyaluronic acid
Assignee: Select Medical Systems Inc
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Filing: 1986-10-17
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Granted: 1989-02-14
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Expires: 2006-10-17
Abstract
Abstract The present invention relates to a process and means for separating motile sperm cells in vitro from an aqueous sperm-containing sample by means of contacting said sample with a layer of an aqueous artificial penetration medium for sperm cells, said medium having incorportated therein a water-soluble physiologically acceptable salf of hyaluronic acid, whereby motile sperm cells are caused to migrate into said layer from said sperm-containing sample. The invention also comprises fertilization with sperm cells separated in this manner.
Inventors
- 👤 Per O. Bergman
- 👤 Yvonne M. Steen
- 👤 Bjorn G. Ingelman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 17:48
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0608; C12N5/0612; A; A61; A61K; A61K35/00; A61K35/12; A61K35/48; A61K35/52
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
66 words
In a process for separating motile sperm cells in vitro from an aqueous sperm-containing sample that involves contacting said sample with a layer of an aqueous artificial penetration medium for sperm cells, whereby motile sperm cells are caused to migrate from said sperm-containing sample into said layer, the improvement comprising that a water-soluble physiologically acceptable salt of hyaluronic acid has been incorporated in said penetration medium.
Claim 2
Dependent Claim
66 words
In a process for separating motile sperm cells in vitro from an aqueous sperm-containing sample that involves contacting said sample with a layer of an aqueous artificial penetration medium for sperm cells, whereby motile sperm cells are caused to migrate from said sperm-containing sample into said layer, the improvement comprising that a water-soluble physiologically acceptable salt of hyaluronic acid has been incorporated in said penetration medium.
Claim 3
Dependent Claim
26 words
A process according to claim 1 wherein the sperm cells which have migrated into said layer of artificial penetration medium are separated and recovered for fertilization.
Claim 4
Independent Claim
38 words
A method for fertilization which comprises using in the fertilization procedure a sperm sample from an artificial penetration medium containing a water-soluble physiologically acceptable salt of hyaluronic acid, into said motile sperm cells have been caused to migrate.
Claim 5
Independent Claim
38 words
A method for fertilization which comprises using in the fertilization procedure a sperm sample from an artificial penetration medium containing a water-soluble physiologically acceptable salt of hyaluronic acid, into said motile sperm cells have been caused to migrate.
References Cited (Backward Citations)
US4327177A
Cited
US4007087A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-02-14 | 1992-08-15 | 1993-02-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-02-14 | 1996-08-15 | 1997-02-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-02-14 | 2000-08-15 | 2001-02-14 | $7700.00 | Window Closed / Lapsed |