US4801504A
Expired / Lapsed
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Fluorescent labels having a polysaccharide bound to polymeric particles
Assignee: Clinical Diagnostic Systems Inc
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Filing: 1987-09-24
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Granted: 1989-01-31
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Expires: 2007-09-24
Abstract
Abstract Fluorescent labels comprise a polysaccharide bound to a polymeric particle which contains a fluorescent rare earth chelate. These labels can be attached to any of a variety of physiologically reactive species to provide labeled species which have improved stability in aqueous solutions. The labeled species are particularly useful in specific binding assays to determine an immunologically reactive ligand, e.g. a hapten, in either solution or dry analytical techniques.
Inventors
- 👤 Brent A. Burdick
- 👤 Susan J. Danielson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 18:32
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/582; G01N33/52; G01N33/525; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/585; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/805; Y10S436/807; Y10S436/808; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
52 words
A fluorescent label comprising a polysaccharide bound to a polymeric particle which is derived from a loadable latex having a discontinuous phase which consists essentially of a polymer prepared from one or more ethylenically unsaturated polymerizable monomers, and an aqueous phase, said polymeric partilce having incorporated therein a fluorescent rare earth chelate.
Claim 2
Dependent Claim
52 words
A fluorescent label comprising a polysaccharide bound to a polymeric particle which is derived from a loadable latex having a discontinuous phase which consists essentially of a polymer prepared from one or more ethylenically unsaturated polymerizable monomers, and an aqueous phase, said polymeric partilce having incorporated therein a fluorescent rare earth chelate.
Claim 3
Dependent Claim
28 words
The label of claim 1 wherein said polysaccharide is a dextran or lipopolysaccharide and has a molecular weight of from about 10 4 to about 10 7 daltons.
Claim 4
Dependent Claim
26 words
The label of claim 1 wherein the chelate is a chelate of europium or terbium and a 1,3-diketone, phthalate, naphthoate, dipyridine, terpyridine or phenanthroline chelating agent.
Claim 5
Dependent Claim
116 words
The label of claim 1 wherein said polymer comprises (a) from 0 to 100 weight percent of units derived from a styrene or vinyl naphthyl monomer, (b) from 0 to about 90 weight percent of units derived from an ethylenically unsaturated monomer of the structure ##STR5## wherein R is hydrogen or alkyl, R 1 is hydrogen or methyl, R 2 is hydrogen, halogen, alkyl, cyano, ##STR6## wherein R 3 is hydrogen or R 4 , and R 4 is an aliphatic group, and (c) from 0 to about 10 weight percent of units derived from an ethylenically unsaturated monomer containing one or more sulfonic acid or carboxy groups, or an ammonium or alkali metal salt thereof.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-01-31 | 1992-08-01 | 1993-01-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-01-31 | 1996-08-01 | 1997-01-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-01-31 | 2000-08-01 | 2001-01-31 | $7700.00 | Window Closed / Lapsed |