US4797361A
Expired / Lapsed
US
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Microorganism and process
Assignee: Lehigh University
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Filing: 1987-09-11
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Granted: 1989-01-10
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Expires: 2007-09-11
Abstract
Abstract This invention includes a process for producing and using increased quantities of extracellular glycoprotein (particularly cellulolytic) enzymes by permitting the enzyme-producing microbial strain to undergo a preliminary growth phase, preferably at about 37 DEG C., and then an enzyme-secretion phase. Also included is a specific microbial strain, RL-P37 of Trichoderma reesei, which grows well at 37 DEG C. and the cellulasic products thereof.
Inventors
- 👤 Bland S. Montenecourt
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 22:41
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/14; C12N1/145; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2437; C12Y; C12Y302/00; C12Y302/01; C12Y302/01004; C12R; C12R2001/00; C12R2001/645; C12R2001/885; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/945
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
8 words
A biologically pure culture of Trichoderma reesei RL-P37.
Claim 2
Dependent Claim
8 words
A biologically pure culture of Trichoderma reesei RL-P37.
Claim 3
Dependent Claim
41 words
A process utilizing Trichoderma reesei RL-P37 for the production of an extracellular glycoprotein enzyme, from the group consisting of carbohydrases, proteases, lipases, and nucleases, said process comprising culturing the microbial strain RL-P37 and permitting said cultured strain to secrete said enzyme.
Claim 4
Dependent Claim
41 words
A process utilizing Trichoderma reesei RL-P37 for the production of an extracellular glycoprotein enzyme, from the group consisting of carbohydrases, proteases, lipases, and nucleases, said process comprising culturing the microbial strain RL-P37 and permitting said cultured strain to secrete said enzyme.
Claim 5
Dependent Claim
42 words
A process utilizing Trichoderma reesei RL-P37 for the production of an extracellular glycoprotein enzyme, from the group consisting of cellulases, laminarinases, amylase, xylanases and pectinases, said process comprising culturing the microbial strain RL-P37 and permitting said cultured strain to secrete said enzyme.
Claim 6
Dependent Claim
42 words
A process utilizing Trichoderma reesei RL-P37 for the production of an extracellular glycoprotein enzyme, from the group consisting of cellulases, laminarinases, amylase, xylanases and pectinases, said process comprising culturing the microbial strain RL-P37 and permitting said cultured strain to secrete said enzyme.
Claim 7
Dependent Claim
55 words
A process utilizing Trichoderma reesei RL-P37 for the production of at least one cellulolytic enzyme, from the group consisting of exocellobiohydrolase, endoglucanase, β-glucosidase, and cellobiase, said process comprising culturing the microbial strain, RL-P37, on a carbon-containing inducing substrate and then permitting said cultured strain in the presence of said inducing substrate to secrete said enzyme.
Claim 8
Dependent Claim
55 words
A process utilizing Trichoderma reesei RL-P37 for the production of at least one cellulolytic enzyme, from the group consisting of exocellobiohydrolase, endoglucanase, β-glucosidase, and cellobiase, said process comprising culturing the microbial strain, RL-P37, on a carbon-containing inducing substrate and then permitting said cultured strain in the presence of said inducing substrate to secrete said enzyme.
Claim 9
Dependent Claim
54 words
The process of claim 4, wherein said carbon-containing inducing substrate is one or more members of the group consisting of cellulose, cellulose derivatives, steam expanded cellulose, β-linked glucosides, cellobiose, wood, wood products, paper, cellulosic agricultural products, cellobiose octaacetate, cellulose mono-, di- or triacetate, carboxymethyl cellulose, hydroxyethyl cellulose, carboxymethyl hydroxyethyl cellulose, esculin or hemicellulosic material.
Claim 10
Dependent Claim
12 words
The process of claim 5, wherein said substrate is steam exploded wood.
Claim 11
Dependent Claim
44 words
A process for treating a cellulosic mass comprising: (1) producing cellulolytic enzymes by a process as recited in claim 5; (2) contacting said secreted cellulolytic enzymes from step (1) with a cellulosic mass to be treated until the glucan content thereof is substantially hydrolyzed.
Claim 12
Dependent Claim
18 words
A process as recited in claim 7, wherein said substrate and said cellulosic mass is steam exploded wood.
Claim 13
Dependent Claim
43 words
A process as recited in any one of claims 2 through 8, wherein said strain is first cultured at a temperature of about 37° C. and then said cultured strain is permitted to secrete said enzyme at an optimal temperature for said secretion.
Claim 14
Dependent Claim
37 words
A process as recited in any one of claims 4 though 8 wherein said strain is first cultured at about 37° C. and then said cultured strain is permitted to secrete said enzyme at about 28° C.
References Cited (Backward Citations)
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1992-01-10 | 1992-07-11 | 1993-01-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-01-10 | 1996-07-11 | 1997-01-10 | $3760.00 | Window Closed / Lapsed |
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