US4472504A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Hyperproducing cellulase microorganism
Assignee: United States Department of the Army
•
Filing: 1983-03-28
•
Granted: 1984-09-18
•
Expires: 2003-03-28
Abstract
Abstract A process and a microorganism for synthesizing cellulase enzymes and solu proteins are described. The microorganism is a mutant strain of an Ascomycete fungus capable of the synthesizing cellulases and soluble proteins in the presence of a growth medium containing cellulose, cellulose enzymic hydrolysate sugars or lactose. The mutant strain is identified as T. reesei MCG80.
Inventors
- 👤 Benedict J. Gallo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 15:31
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/22; C12N1/14; C12N1/145; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2437; C12N9/2445; C12Y; C12Y302/00; C12Y302/01; C12Y302/01004; C12Y302/01021; C12R; C12R2001/00; C12R2001/645; C12R2001/885; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/945
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
9 words
A biologically pure culture of T. reesei strain MCG80.
Claim 2
Dependent Claim
9 words
A biologically pure culture of T. reesei strain MCG80.
Claim 3
Dependent Claim
18 words
A biologically pure culture according to claim 1 wherein said culture has the capability to synthesize cellulase enzymes.
Claim 4
Dependent Claim
28 words
A biologically pure culture according to claim 2 wherein the synthesis of the cellulase enzymes by the organism is induced by cellulose, cellulose enzymic hydrolysate sugars or lactose.
Claim 5
Dependent Claim
50 words
A process for producing enzymes capable of catalyzing the hydrolysis of cellulose comprising: (a) inoculating a suitable sterile growth medium with T. reesei strain MCG80, said growth medium containing an inducer of cellulase synthesis, and (b) incubating the inoculated growth medium under conditions which will permit the growth of MCG80.
Claim 6
Dependent Claim
50 words
A process for producing enzymes capable of catalyzing the hydrolysis of cellulose comprising: (a) inoculating a suitable sterile growth medium with T. reesei strain MCG80, said growth medium containing an inducer of cellulase synthesis, and (b) incubating the inoculated growth medium under conditions which will permit the growth of MCG80.
Claim 7
Dependent Claim
14 words
A process according to claim 4 wherein the inducer of cellulase synthesis is cellulose.
Claim 8
Dependent Claim
30 words
A process according to claim 5 wherein the pH is maintained at 3.75 for the first 48 hours of incubation and then at 3.5 for the remainder of the incubation.
Claim 9
Dependent Claim
14 words
A process according to claim 4 wherein the inducer of cellulase synthesis is lactose.
Claim 10
Dependent Claim
16 words
A process according to claim 7 wherein the pH is maintained at 3.5 during the incubation.
Claim 11
Dependent Claim
59 words
A process for producing soluble protein from cellulose, cellulose enzymic hydrolysate sugar or lactose substrate which comprises: (a) inoculating sterile growth medium containing a substrate selected from the group consisting of cellulose, cellulose enzymic hydrolysate sugar and lactose with T. reesei strain MCG80, and (b) incubating the inoculated growth under conditions which will permit the growth of strain MCG80.
Claim 12
Dependent Claim
59 words
A process for producing soluble protein from cellulose, cellulose enzymic hydrolysate sugar or lactose substrate which comprises: (a) inoculating sterile growth medium containing a substrate selected from the group consisting of cellulose, cellulose enzymic hydrolysate sugar and lactose with T. reesei strain MCG80, and (b) incubating the inoculated growth under conditions which will permit the growth of strain MCG80.
Claim 13
Dependent Claim
35 words
A process according to claim 9 wherein the substrate is cellulose and wherein the pH is maintained at 3.75 for the first 48 hours of incubation and at 3.5 for the remainder of the incubation.
Claim 14
Dependent Claim
21 words
A process according to claim 9 wherein the substrate is lactose and wherein the pH is maintained at 3.5 during incubation.
References Cited (Backward Citations)
SU591502A1
Cited
US4275163A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-09-18 | 1988-03-19 | 1988-09-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-09-18 | 1992-03-19 | 1992-09-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-09-18 | 1996-03-19 | 1996-09-18 | $7700.00 | Window Closed / Lapsed |