Miniaturized column chromatography separation apparatus and method of assaying biomolecules employing the same

Claim 2

A miniaturized column chromatography apparatus, comprising: a generally tubular column member having a plurality of chambers, each of said plurality of chambers being disposed in fluid flow communication with another of said plurality of chambers, said tubular column member further having an eluent inlet and eluate outlet, said inlet having a relatively larger diameter than and in fluid flow communication with said eluate outlet, said eluate outlet further having a generally frusto-conical shape; a planar disc-shaped porous filter horizontally disposed within said generally tubular column member and subtending said eluate outlet; chromatographic separatory medium packed within said generally tubular column member, said chromatographic separatory medium being selected dependent upon the materials to be separated; vented capping means for closing said eluate inlet disposed within eluate inlet opening and comprising a cap and a cap adapter, wherein said cap further comprises a generally cylindrical hollow lower stalk portion and a generally planar upper cap portion having a lower ring portion depending therefrom, wherein said lower ring portion has an inside diameter corresponding to an outside diameter of an upper rim portion of said generally tubular column member; and wherein said cap adapter further comprises a generally cylindrical hollow upper portion, a generally cylindrical hollow lower portion depending therefrom and a radial flange extending outwardly from an upper surface of said generally cylindrical lower portion, said upper portion having outside diameter corresponding to an inner diameter of said generally cylindrical hollow lower stalk portion of said cap and said lower portion having an outside diameter corresponding to an inside diameter of an upper portion of said generally tubular column member; and an eluate receiving member having a rounded bottom portion for forming a pellet therein; said eluate receiving member being disposed in fluid communication with said generally tubular column member.

Claim 3

The miniaturized column chromatography apparatus according to claim 1, wherein each of said plurality of chambers further comprising an outlet and an inlet opening thereto, wherein each of said outlet openings has a relatively smaller diameter than each of said associated inlet openings.

Claim 4

The miniaturized column chromatography apparatus according to claim 2, wherein said generally tubular column member further a unitary column member constructed of a chemically and thermally inert plastic material.

Claim 5

The miniaturized column chromatography apparatus according to claim 2, wherein said generally tubular column member further comprises a unitary column member constructed of an ethylene and propylene plastic.

Claim 6

The miniaturized column chromatography apparatus according to claim 2, wherein each of said outlet openings further comprises a porous filter subtending said outlet opening.

Claim 7

The miniaturized column chromatography apparatus according to claim 5, wherein each of said plurality of chambers further comprises different chromatographic separatory media packed therein, each of said different chromatographic separatory media being selected dependent upon the materials to be separated.

Claim 8

The miniaturized column chromatography apparatus according to claim 6, wherein said chromatographic separatory medium is selected from the group consisting of ion exchange resin, ion exchange cellulose, ion exchange gel, sephadex, silica gel, alumina, hydroxyapatite gel, phenylborate cellulose and affinity gel.

Claim 9

The miniaturized column chromatography apparatus according to claim 2, wherein said chromatographic separatory medium is selected from the group consisting of ion exchange resin, ion exchange cellulose, ion exchange gel, sephadex, silica gel, alumina, hydroxyapatite gel, phenylborate cellulose and affinity gel.

Claim 10

The miniaturized column chromatography apparatus according to claim 1, wherein said generally tubular column member further comprises a unitary column member constructed of a chemically and thermally inert plastic material.

Claim 11

The miniaturized column chromatography apparatus according to claim 1, wherein said generally tubular column member further comprises a unitary column member constructed of an ethylene and propylene plastic.

Claim 12

The miniaturized column chromatography apparatus according to claim 1, wherein said porous filter further comprises a polymeric plastic material.

Claim 13

The miniaturized column chromatography apparatus according to claim 1, wherein said porous filter further comprises a polymeric plastic material selected from the group consisting of polyethylene, polypropylene, polyether-polyurethane, polyester-polyurethane, polyolefin, polyvinylidene flouride, ethylene-vinyl acetate, styrene-acrylonitrile and polytetrafluoroethylene.

Claim 14

The miniaturized column chromatography apparatus according to claim 1, wherein said generally tubular column member further comprises a plurality of said porous filters horizontally disposed therein forming a plurality of chambers therebetween.

Claim 15

The miniaturized column chromatography apparatus according to claim 13, wherein each of said plurality of chambers further comprises different chromatographic separatory media packed therein, each of said different chromatographic separatory media being selected dependent upon the materials to be separated.

Claim 16

The miniaturized column chromatography apparatus according to claim 1, wherein each of said chambers further comprises a porous filter horizontally disposed within said chamber and subtending an outlet opening thereof.

Claim 17

The miniaturized column chromatography apparatus according to claim 15, wherein each of said plurality of chambers further comprises different chromatographic separatory media packed therein, each of said different chromatographic separatory media being selected dependent upon the materials to be separated.

Claim 18

The miniaturized column chromatography apparatus according to claim 1, wherein said chromatographic separatory medium is selected from the group consisting of ion exchange resin, ion exchange cellulose, ion exchange gel, sephadex, silica gel, alumina, hydroxyapatite gel, phenylborate cellulose and affinity gel.

Claim 19

The miniaturized column chromatography apparatus according to claim 1, wherein said multi-stage column member further comprises a plurality of said porous polymeric filters subtending an outlet opening of at least one of said chambers of said multi-stage column member.

Claim 20

The miniaturized column chromatography apparatus according to claim 18, wherein each of said plurality of chambers having an outlet opening subtended by said porous polymeric filters further comprises different chromatographic separatory media packed therein, each of said different chromatographic separatory media being selected dependent upon the materials to be separated.

Claim 21

A miniaturized column chromatography apparatus, comprising, in combination: a multi-stage column member having an eluate inlet and eluent outlet opening and a plurality of chambers in fluid flow communication disposed therebetween, each of said chambers having an inlet and outlet opening, each of said inlet openings further having a relatively larger diameter than a communicating outlet opening, said eluate outlet further having a generally frustro-conical shape and a central bore passing therethrough; at least one of a plurality of porous polymeric disc-shaped filters horizontally disposed within said multi-stage column member and subtending said eluate outlet and at least one of said outlet openings of said plurality of chambers; at least one of a plurality of chromatographic separatory media packed within said generally tubular generally tubular column member, said chromatographic separatory media being selected dependent upon the materials to be separated wherein a different and distinct one of said at least one of a plurality of chromatographic separatory media is packed in each of said plurality of chambers of said multi-stage column member for separating different and distinct materials from a solution; a cap member having a generally cylindrical hollow lower stalk portion and a generally planar upper cap portion having a lower ring portion depending therefrom, wherein said lower ring portion has an inside diameter corresponding to an outside diameter of an upper rim portion of said generally tubular column member; a cap adapter having a generally cylindrical hollow upper portion, a generally cylindrical hollow lower portion depending therefrom and a radial flange extending outwardly from an upper surface of said generally cylindrical lower portion, said upper portion having outside diameter corresponding to an inner diameter of said generally cylindrical hollow lower stalk portion of said cap and said lower portion having an outside diameter corresponding to an inside diameter of an upper portion of said generally tubular column member; and an eluate receiving member disposed in fluid communication with said generally tubular column member.

Claim 22

A miniaturized column chromatography apparatus, comprising, in combination: a multi-stage column member having an eluate inlet and eluent outlet opening and a plurality of chambers in fluid flow communication disposed therebetween, each of said chambers having an inlet and outlet opening, each of said inlet openings further having a relatively larger diameter than a communicating outlet opening, said eluate outlet further having a generally frustro-conical shape and a central bore passing therethrough; at least one of a plurality of porous polymeric disc-shaped filters horizontally disposed within said multi-stage column member and subtending said eluate outlet and at least one of said outlet openings of said plurality of chambers; at least one of a plurality of chromatographic separatory media packed within said generally tubular generally tubular column member, said chromatographic separatory media being selected dependent upon the materials to be separated wherein a different and distinct one of said at least one of a plurality of chromatographic separatory media is packed in each of said plurality of chambers of said multi-stage column member for separating different and distinct materials from a solution; a cap member having a generally cylindrical hollow lower stalk portion and a generally planar upper cap portion having a lower ring portion depending therefrom, wherein said lower ring portion has an inside diameter corresponding to an outside diameter of an upper rim portion of said generally tubular column member; a cap adapter having a generally cylindrical hollow upper portion, a generally cylindrical hollow lower portion depending therefrom and a radial flange extending outwardly from an upper surface of said generally cylindrical lower portion, said upper portion having outside diameter corresponding to an inner diameter of said generally cylindrical hollow lower stalk portion of said cap and said lower portion having an outside diameter corresponding to an inside diameter of an upper portion of said generally tubular column member; and an eluate receiving member disposed in fluid communication with said generally tubular column member.

Claim 23

The miniaturized column chromatography apparatus according to claim 20, wherein said generally tubular column member further comprises a unitary column member constructed of a chemically and thermally inert plastic material.

Claim 24

The miniaturized column chromatography apparatus according to claim 20, wherein said porous polymeric filter further comprises a plastic material selected from the group consisting of polyethylene, polypropylene, polyether-polyurethane, polyester-polyurethane, polyolefin, polyvinylidene fluoride, ethylene-vinyl acetate, styrene-acrylonitrile and polytetrafluoroethylene.

Claim 25

The miniaturized column chromatography apparatus according to claim 20, wherein said chromatographic separatory medium is selected from the group consisting of ion exchange resin, ion exchange cellulose, ion exchange gel, sephadex, silica gel, alumina, hydroxyapatite gel, phenylborate cellulose and affinity gel.

Claim 26

The miniaturized column chromatography apparatus according to claim 20, wherein said cap portion of said capping means further comprises venting means disposed on said cap portion for alleviating negative pressure within said generally tubular column member during a separation run.