US4782046A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Ultrapure hyaluronic acid and method of making it
Assignee: Bayer Corp
•
Filing: 1986-09-18
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Granted: 1988-11-01
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Expires: 2006-09-18
Abstract
Abstract Hyaluronic acid having controlled molecular weight and substantially free of protein and nucleic acids can be prepared from a hyaluronidase-negative or hyaluronidase inhibited microbiological source. Product may be used as replacement fluid in mammals.
Inventors
- 👤 Karen K. Brown
- 👤 Linda L. C. Ruiz
- 👤 Ivo Van De Rijn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 12:53
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/04; C12P19/26; A; A61; A61K; A61K31/00; A61K31/70; A61K31/715; A61P; A61P7/00; C08; C08B; C08B37/00; C08B37/006; C08B37/0063; C08B37/0072
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
69 words
A sterile preparation comprising hyaluronic acid or a salt thereof, the preparation having a protein content of less than about 1.25 mg/ml and a nucleic acid content of less than about 45 μg/ml and having a molecular weight range such that at least 98% of the preparation is within a molecular weight range of from about 2.0 million to about 4.0 million as determined by High Performance Liquid Chromatography.
Claim 2
Dependent Claim
69 words
A sterile preparation comprising hyaluronic acid or a salt thereof, the preparation having a protein content of less than about 1.25 mg/ml and a nucleic acid content of less than about 45 μg/ml and having a molecular weight range such that at least 98% of the preparation is within a molecular weight range of from about 2.0 million to about 4.0 million as determined by High Performance Liquid Chromatography.
Claim 3
Dependent Claim
23 words
The preparation of claim 1 wherein the preparation, when subject to High Performance Liquid Chromatography, produces an essentially single, substantially symmetrical distribution peak.
Claim 4
Dependent Claim
26 words
The preparation of claim 2 wherein the protein content is less than about 0.10 mg/ml and the nucleic acid content is less than about 5 μg/ml.
Claim 5
Dependent Claim
24 words
The preparation of claim 3 wherein the preparation has a Clinical Index of less than 6.0 as determined by the Horse Joint Reactivity Test.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-11-01 | 1992-05-02 | 1992-11-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-11-01 | 1996-05-02 | 1996-11-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-11-01 | 2000-05-02 | 2000-11-01 | $7700.00 | Window Closed / Lapsed |