US4775483A
Expired / Lapsed
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Method to reduce plasma cholesterol
Assignee: Memorial University of Newfoundland
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Filing: 1986-09-18
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Granted: 1988-10-04
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Expires: 2006-09-18
Abstract
Abstract Cholesterol in liquids such as blood plasma can be reduced by passing such liquids over an insoluble matrix such as agarose or treated glass beads to which is attached a C-reactive protein capable of binding cholesterol. An eluent such as sodium chloride or phosphorylcholine can be used to remove bound cholesterol and regenerate the insoluble matrix-C-reactive protein combination for re-use.
Inventors
- 👤 Sailen S. Mookerjea
- 👤 Arun Nagpurkar
- 👤 Uday Saxena
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 03:38
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01D15/3809; B01J; B01J20/00; B01J20/28; B01J20/28002; B01J20/28004; B01J20/30; B01J20/3092; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3206; B01J20/3208; B01J20/3212; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3248; B01J20/3251; B01J20/3257; B01J20/3259; B01J20/3261; B01J20/3268; B01J20/3272; B01J20/3274; B01J20/34; B01J20/3425; B01J20/345; B01J20/3475; B01D15/3814; B01D15/42; B01D15/424; B01D15/426; B01J2220/00; B01J2220/50; B01J2220/58
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
54 words
A method to separate cholesterol from a cholesterol-containing liquid comprising passing said cholesterol-containing liquid over a selected insoluble matrix upon which is immobilized a C-reactive protein capable of binding said cholesterol or cholesterol-containing material thereby to bind said cholesterol or cholesterol-containing material and separating a liquid with a reduced cholesterol concentration from said matrix.
Claim 2
Independent Claim
54 words
A method to separate cholesterol from a cholesterol-containing liquid comprising passing said cholesterol-containing liquid over a selected insoluble matrix upon which is immobilized a C-reactive protein capable of binding said cholesterol or cholesterol-containing material thereby to bind said cholesterol or cholesterol-containing material and separating a liquid with a reduced cholesterol concentration from said matrix.
Claim 3
Dependent Claim
32 words
The method of claim 1 wherein said cholesterol-containing liquid is blood plasma and said insoluble matrix is selected from the group consisting of an activated agarose support and an activated glass support.
Claim 4
Dependent Claim
16 words
The method of claim 2 wherein the activated glass support is a diisothiocyanate activated glass support.
Claim 5
Dependent Claim
30 words
The method of claim 1 wherein said cholesterol-containing liquid is blood plasma and said C-reactive protein is selected from the group consisting of rat C-reactive protein and hamster C-reactive protein.
Claim 6
Dependent Claim
13 words
The method of claim 1 wherein the cholesterol-containing material comprises low density lipoproteins.
Claim 7
Dependent Claim
16 words
The method of claim 1 wherein the cholesterol-containing material is apo B or apo E apoprotein.
Claim 8
Dependent Claim
32 words
The method of claim 1 followed by treating the insoluble matrix-C-reactive protein-cholesterol combination with an eluent to elute the cholesterol and regenerate the insoluble matrix to which is linked a C-reactive protein.
Claim 9
Dependent Claim
15 words
The method of claim 7 wherein the eluent is an aqueous solution of sodium chloride.
Claim 10
Dependent Claim
23 words
The method of claim 8 wherein the aqueous solution of sodium chloride is in the form of a concentration gradient up to 0.5M.
Claim 11
Dependent Claim
10 words
The method of claim 7 wherein the eluent is phosphorylcholine.
References Cited (Backward Citations)
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