US4476093A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Kit for preparing sample for use in endotoxin test
Assignee: Tanabe Pharma Corp
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Filing: 1983-04-28
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Granted: 1984-10-09
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Expires: 2003-04-28
Abstract
Abstract A method for preparing a sample for use in endotoxin test which comprises subjecting a sample of plasma or blood preparation to a contact with water-insoluble anti human alpha 2-macroglobulin antibody and to a gel filtration by means of a gel of allyl dextran crosslinked with N,N'-methylenebis acrylamide or a gel of polyvinyl alcohol having many hydrophilic hydroxyl groups, and recovering the maximum molecular weight fraction. A kit for preparing a sample for use in the endotoxin test consisting of a set of a sample-developing column prepared by packing a water-insoluble anti-human alpha 2-macroglobulin antibody and a gel of allyl dextran crosslinked with N,N'-methylenebis acrylamide or a gel of polyvinyl alcohol having many hydrophilic hydroxyl groups, both equilibrated with a buffer solution of pH 7.0-7.4, into a column and tightly sealing the column, an aqueous solution of a high molecular weight colored substance to be added to the sample and a buffer solution for use in developing the sample.
Inventors
- 👤 Ryozo Watanabe
- 👤 Keiichi Kawasumi
- 👤 Shoichi Maeda
- 👤 Takashi Shoji
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 02:59
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/96; G01N33/53; G01N33/531; G01N33/579; G01N2333/00; G01N2333/81; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/825
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
113 words
A kit for preparing a sample for use in an endotoxin test consisting of: a sample-developing column prepared by packing a water-insoluble anti-human α 2 -macroglobulin antibody and a gel of allyl dextran cross-linked with N,N'-methylenebis acrylamide or a gel of polyvinyl alcohol having many hydrophilic hydroxyl groups as a carrier for gel filtration, both equilibrated with a buffer solution of pH 7.0-7.4, into a column and tightly sealing the column, an aqueous solution containing a high molecular weight colored substance which serves as an endotoxin marker and has an average molecular weight of 300,000 or higher to be added to the sample, and a buffer solution for use in developing the sample.
Claim 2
Dependent Claim
113 words
A kit for preparing a sample for use in an endotoxin test consisting of: a sample-developing column prepared by packing a water-insoluble anti-human α 2 -macroglobulin antibody and a gel of allyl dextran cross-linked with N,N'-methylenebis acrylamide or a gel of polyvinyl alcohol having many hydrophilic hydroxyl groups as a carrier for gel filtration, both equilibrated with a buffer solution of pH 7.0-7.4, into a column and tightly sealing the column, an aqueous solution containing a high molecular weight colored substance which serves as an endotoxin marker and has an average molecular weight of 300,000 or higher to be added to the sample, and a buffer solution for use in developing the sample.
Claim 3
Dependent Claim
34 words
A kit according to claim 1, wherein the diameter of the column is 7-12 mm and the water-insoluble anti-human α 2 -macroglobulin antibody and the gel are charged in an amount of 2-4 ml.
Claim 4
Dependent Claim
25 words
A kit according to claim 1, wherein said water-insoluble anti-human α 2 -macroglobulin antibody exists in a proportion of 5-20% W/W in the packing material.
Claim 5
Dependent Claim
19 words
A kit according to claim 1, wherein the network structure of the carrier for gel filtration is 100-300 mesh.
Claim 6
Dependent Claim
56 words
A kit according to claim 1, wherein said anti-human α 2 -macroglobulin antibody is a product obtained by administering α 2 -macroglobulin as an immunogen to guinea pig, rat, hamster, sheep, horse or house rabbit, and after the immunization, recovering the antiserum, separating the anti-human α 2 -macroglobulin antibody from said antiserum and purifying the antibody.
Claim 7
Dependent Claim
19 words
A kit according to claim 1, wherein said high molecular colored substance is dextran substituted with Cibacron Blue F3G-A.
Claim 8
Dependent Claim
13 words
A kit according to claim 1, wherein said buffer solution contains a bactericide.
Claim 9
Dependent Claim
11 words
A kit according to claim 7, wherein said bactericide is bromonitroalcohol.
Claim 10
Dependent Claim
31 words
A kit according to claim 1, wherein said water-insoluble anti-human α 2 -macroglobulin antibody is a product obtained by chemically combining an anti-human α 2 -macroglobulin antibody with a water-insoluble carrier.
Claim 11
Dependent Claim
20 words
A kit according to claim 9, wherein the water-insoluble carrier is the same as the carrier for the gel filtration.
Claim 12
Dependent Claim
27 words
A kit according to claim 9, wherein said water-insoluble carrier is activated polyacrylamide gel, agarose, crosslinked dextran, allyl dextran crosslinked with N,N'-methylenebis acrylamide or polyvinyl alcohol gel.
Claim 13
Dependent Claim
23 words
A kit according to claim 11, wherein said water-insoluble carrier is activated allyl dextran crosslinked with N,N'-methylenebis acrylamide or activated polyvinyl alcohol gel.
Claim 14
Dependent Claim
17 words
A kit according to claim 11 or 12, wherein said water-insoluble carrier is activated with cyanogen bromide.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-10-09 | 1988-04-09 | 1988-10-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-10-09 | 1992-04-09 | 1992-10-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-10-09 | 1996-04-09 | 1996-10-09 | $7700.00 | Window Closed / Lapsed |