US4774323A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Purification of von Willebrand Factor solutions using gel permeation chromatography
Assignee: Armour Pharmaceutical Co
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Filing: 1987-06-29
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Granted: 1988-09-27
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Expires: 2007-06-29
Abstract
Abstract A method of preparing von Willebrand Factor by disassociating it from a chaotropic agent in solution therewith and preferably treating the same under controlled temperature either in liquid or lyophilized form.
Inventors
- 👤 Jack Newman
- 👤 David L. Farb
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 22:53
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/755; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
108 words
A method of preparing von Willebrand Factor from a freshly obtained aqueous solution containing von Willebrand Factor and a 0.05 to 5M solution of a chaotropic agent comprising the steps of: separating the von Willebrand Factor from the chaotropic agent by transferring said freshly obtained aqueous solution onto a desalting column to effect adsorption of said chaotropic agent thereto; eluting said von Willebrand Factor therefrom with a buffer solution containing from about 0.01 to about 0.5M NaCl and about 1 to about 10 mM of histidine and having a pH of 6.5 to 7.5; sterile filtering said von Willebrand Factor/buffer solution; and lyophilizing said von Willebrand Factor/buffer solution.
Claim 2
Independent Claim
108 words
A method of preparing von Willebrand Factor from a freshly obtained aqueous solution containing von Willebrand Factor and a 0.05 to 5M solution of a chaotropic agent comprising the steps of: separating the von Willebrand Factor from the chaotropic agent by transferring said freshly obtained aqueous solution onto a desalting column to effect adsorption of said chaotropic agent thereto; eluting said von Willebrand Factor therefrom with a buffer solution containing from about 0.01 to about 0.5M NaCl and about 1 to about 10 mM of histidine and having a pH of 6.5 to 7.5; sterile filtering said von Willebrand Factor/buffer solution; and lyophilizing said von Willebrand Factor/buffer solution.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-09-27 | 1992-03-28 | 1992-09-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-09-27 | 1996-03-28 | 1996-09-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-09-27 | 2000-03-28 | 2000-09-27 | $7700.00 | Window Closed / Lapsed |