US4767670A
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Chromatographic supports for separation of oligonucleotides
Assignee: EIDP Inc
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Filing: 1987-01-21
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Granted: 1988-08-30
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Expires: 2007-01-21
Abstract
Abstract A chromatographic packing useful for the separation of oligonucleotides is disclosed. The packing includes an insert porous support particle and a silane bonded phase comprising a weak anion exchange group in close proximity to at least one polar non-ionic group.
Inventors
- 👤 Geoffrey B. Cox
- 👤 Anthony Atkinson
- 👤 Peter A. D. Edwardson
- 👤 Michael D. Scawen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 12:19
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/281; B01J20/286; B01J20/288; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/32; B01D15/325; B01D15/36; B01D15/361; B01J20/28; B01J20/28002; B01J20/28004; B01J20/30; B01J20/3071; B01J20/3078; B01J20/3092; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3206; B01J20/3208; B01J20/321; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3257; B01J20/3259; B01J20/3263; B01J20/3285; B01J41/00; B01J41/04; B01J41/07; B01J41/20; B01J2220/00; B01J2220/50; B01J2220/54; Y; Y10; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2993; Y10T428/2995; Y10T428/2996; Y10T428/2998
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
69 words
A packing for chromatographic separations comprising inert support particles with a silane bonded phase wherein the bonded phase contains (a) a weak anion exchange group consisting of either --(NH)-- or ##STR9## and at least two polar non-ionic groups each within three backbone chain atoms of the weak anion exchange group, (b) contains hydrophobic regions, and (c) the silane surface coverage of the particle is more than about 0.9 micromole/m2.
Claim 2
Dependent Claim
69 words
A packing for chromatographic separations comprising inert support particles with a silane bonded phase wherein the bonded phase contains (a) a weak anion exchange group consisting of either --(NH)-- or ##STR9## and at least two polar non-ionic groups each within three backbone chain atoms of the weak anion exchange group, (b) contains hydrophobic regions, and (c) the silane surface coverage of the particle is more than about 0.9 micromole/m2.
Claim 3
Dependent Claim
17 words
The packing set forth in claim 1 wherein the support particles include silica, aluminia, and organic polymers.
Claim 4
Dependent Claim
24 words
The packing set forth in claim 2 wherein the support particles range in size from about 2 μm to about 30 μm in diameter.
Claim 5
Dependent Claim
15 words
The packing set forth in claim 2 wherein the bonded phase has the structure: ##STR10##
Claim 6
Dependent Claim
15 words
The packing set forth in claim 3 wherein the bonded phase has the structure: ##STR11##
Claim 7
Dependent Claim
24 words
The packing set forth in claim 1 wherein the support particles range in size from about 2 μm to about 30 μm in diameter.
Claim 8
Dependent Claim
20 words
The packing of claim 2 wherein the bonded phase has the structure ##STR12## where n is either 2 or 3.
Claim 9
Dependent Claim
19 words
The packing of claim 2 wherein the bonded phase has the structure ##STR13## where n=1-2 and X=alkyl or hydroxyalkyl.
Claim 10
Independent Claim
113 words
A method of separating oligonucleotides by the steps of entraining the oligonucleotides in a mobile liquid phase and chromatographically subjecting the oligonucleotides to a packing by passing the mobile phase through the packing, the packing comprising inert support particles with a solid silane bonded phase wherein the bonded phase contains (a) a weak anion exchange group consisting of either --(NH)-- or ##STR14## and at least two polar non-ionic groups each within three backbone chain atoms of the weak anion exchange group, (b) contains hydrophobic regions, and (c) the silane surface coverage of the particle is more than about 0.9 micromole/m 2 , thereby using both reversed-phase and ion-exchange mechanisms to effect the separation.
Claim 11
Independent Claim
113 words
A method of separating oligonucleotides by the steps of entraining the oligonucleotides in a mobile liquid phase and chromatographically subjecting the oligonucleotides to a packing by passing the mobile phase through the packing, the packing comprising inert support particles with a solid silane bonded phase wherein the bonded phase contains (a) a weak anion exchange group consisting of either --(NH)-- or ##STR14## and at least two polar non-ionic groups each within three backbone chain atoms of the weak anion exchange group, (b) contains hydrophobic regions, and (c) the silane surface coverage of the particle is more than about 0.9 micromole/m 2 , thereby using both reversed-phase and ion-exchange mechanisms to effect the separation.
Claim 12
Dependent Claim
16 words
A method as set forth in claim 9 wherein the bonded phase has the structure: ##STR15##
Claim 13
Dependent Claim
23 words
A method as set forth in claim 9 wherein the bonded phase has the structure: ##STR16## where n is either 2 or 3.
Claim 14
Dependent Claim
21 words
A method as set forth in claim 9 wherein the mobile phase has a pH from about 4.0 to about 8.5.
Claim 15
Independent Claim
88 words
A method of formulating a chromatographic packing for chromatographically separating oligonucleotides comprising the steps of: reacting a reactive silane with a modifying reagent containing a weak anion exchange group and a polar non-ionic group, to provide a bonded stationary phase, wherein said stationary phase (a) contains a weak anion exchange group consisting of either --(NH)-- or ##STR17## and at least two polar non-ionic groups each within three backbone atoms of the weak anion exchange group and (b) contains hydrophobic regions, and reacting the stationary phase with a support.
Claim 16
Independent Claim
88 words
A method of formulating a chromatographic packing for chromatographically separating oligonucleotides comprising the steps of: reacting a reactive silane with a modifying reagent containing a weak anion exchange group and a polar non-ionic group, to provide a bonded stationary phase, wherein said stationary phase (a) contains a weak anion exchange group consisting of either --(NH)-- or ##STR17## and at least two polar non-ionic groups each within three backbone atoms of the weak anion exchange group and (b) contains hydrophobic regions, and reacting the stationary phase with a support.
Claim 17
Dependent Claim
27 words
A method as set forth in claim 13 wherein the modifying reagent is: NH 2 --(CH 2 ) n --OH where n is either 2 or 3.
Claim 18
Dependent Claim
23 words
A method as set forth in claim 14 wherein the reactive silane is γ-glycidoxy propyltrimethoxyl silane and the reagent were n=2 is 2-aminoethanol.
Claim 19
Dependent Claim
20 words
A method as set forth in claim 13 wherein the modifying reagent is ##STR18## where n=1-2 and X=alkyl or hydroxyalkyl.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-08-30 | 1992-02-29 | 1992-08-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-08-30 | 1996-02-29 | 1996-08-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-08-30 | 2000-02-29 | 2000-08-30 | $7700.00 | Window Closed / Lapsed |