US4767610A
Expired / Lapsed
US
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Method for detecting abnormal cell masses in animals
Assignee: University of California
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Filing: 1984-10-19
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Granted: 1988-08-30
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Expires: 2004-10-19
Abstract
Abstract An improved method of detecting abnormal cell mass in animals, particularly tumors, wherein a fluorocarbon emulsion and a lysophosphatide or proteinaceous material are administered to animals causing the fluorocarbon to be taken up by the cell mass in hithertofor unobtainable amounts, thus rendering it readily detectable by a variety of physical or chemical methods.
Inventors
- 👤 David M. Long
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 03:42
CPC Classifications
A; A61; A61K; A61K49/00; A61K49/04; A61K49/0433; A61K49/0447; A61K49/0461; A61K49/0471
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
73 words
A method for enhancing the detection of tumor cell mass in animals, comprising the steps of: forming an emulsion consisting of a radiopaque fluorocarbon at a concentration of about 10-50%, a lysophosphatide, said lysophosphatide being at a concentration of about 0.05-0.5%, and a phosphoglyceride at a concentration of about 1-5%; administering said emulsion in an amount sufficient to cause said emulsion to contact said tumor cell mass; and visualizing said tumor cell mass.
Claim 2
Independent Claim
73 words
A method for enhancing the detection of tumor cell mass in animals, comprising the steps of: forming an emulsion consisting of a radiopaque fluorocarbon at a concentration of about 10-50%, a lysophosphatide, said lysophosphatide being at a concentration of about 0.05-0.5%, and a phosphoglyceride at a concentration of about 1-5%; administering said emulsion in an amount sufficient to cause said emulsion to contact said tumor cell mass; and visualizing said tumor cell mass.
Claim 3
Dependent Claim
23 words
A method as described in claim 1 wherein said fluorocarbon is radiopaque and is selected from the group consisting of perfluorocarbon and perfluoroctylbromide.
Claim 4
Dependent Claim
16 words
A method as described in claim 1 wherein said fluorocarbon is emulsified with phosphatidyl choline phosphoglyceride.
Claim 5
Dependent Claim
20 words
A method as described in claim 1 wherein said tumor cell mass is visualized with X-ray, ultrasound, or magnetic resonance.
Claim 6
Independent Claim
80 words
A method for enhancing the detection of tumor cell mass in animals, comprising the steps of: forming an emulsion of fluorocarbon at a concentration of about 10-50%, said fluorocarbon being radiopaque and selected from the group consisting of perfluorocarbon and perfluoroctylbromide, and a phosphoglyceride at a concentration of about 1-5%, and glucagon; administering said emulsion to animals in amounts sufficient to cause said emulsion to contact said tumor cell mass; and visualizing said emulsion present in said tumor cell mass.
Claim 7
Independent Claim
80 words
A method for enhancing the detection of tumor cell mass in animals, comprising the steps of: forming an emulsion of fluorocarbon at a concentration of about 10-50%, said fluorocarbon being radiopaque and selected from the group consisting of perfluorocarbon and perfluoroctylbromide, and a phosphoglyceride at a concentration of about 1-5%, and glucagon; administering said emulsion to animals in amounts sufficient to cause said emulsion to contact said tumor cell mass; and visualizing said emulsion present in said tumor cell mass.
Claim 8
Dependent Claim
13 words
A method as described in claim 5 wherein said phosphoglyceride is phosphatidyl choline.
Claim 9
Dependent Claim
24 words
A method as described in claim 5 wherein said glucagon present in said emulsion is administered intravenously to said animals in an effective amount.
Claim 10
Dependent Claim
29 words
A method as described in claim 7 wherein said glucagon is administered to animals in one or more amounts of about 10 micrograms to 1 mg/kg of body weight.
Claim 11
Dependent Claim
49 words
A composition for distinguishing tumor cell mass from normal tissue, comprising: a fluorocarbon selected from the group consisting of perfluorocarbon and perfluoroctylbromide, said fluorocarbon being present in about 10-50%; a lysophosphatide at a concentration of about 0.05-0.5%; and an emulsifier being a phosphoglyceride at a concentration of about 1-5%.
Claim 12
Dependent Claim
49 words
A composition for distinguishing tumor cell mass from normal tissue, comprising: a fluorocarbon selected from the group consisting of perfluorocarbon and perfluoroctylbromide, said fluorocarbon being present in about 10-50%; a lysophosphatide at a concentration of about 0.05-0.5%; and an emulsifier being a phosphoglyceride at a concentration of about 1-5%.
Claim 13
Dependent Claim
13 words
A composition as described in claim 9 wherein said phosphoglyceride is phosphatidyl choline.
Claim 14
Dependent Claim
52 words
A contrast composition useful for contrasting tumor cell mass from normal tissue, comprising: a fluorocarbon selected from the group consisting of perfluorocarbon an perfluoroctylbromide, said fluorocarbon being present in an amount of about 10-50%; glucagon present in an effective amount in said composition; and phosphoglyceride present at a concentration of about 1-5%.
Claim 15
Dependent Claim
52 words
A contrast composition useful for contrasting tumor cell mass from normal tissue, comprising: a fluorocarbon selected from the group consisting of perfluorocarbon an perfluoroctylbromide, said fluorocarbon being present in an amount of about 10-50%; glucagon present in an effective amount in said composition; and phosphoglyceride present at a concentration of about 1-5%.
Claim 16
Dependent Claim
13 words
A composition as described in claim 11 wherein said phosphoglyceride is phosphatidyl choline.
Claim 17
Independent Claim
83 words
A method for enhancing the detection of tumor cell mass in animals, comprising the steps of: forming an emulsion of fluorocarbon, said fluorocarbon being at a concentration of about 10-50%, said fluorocarbon being radiopaque and selected from the group consisting of perfluorocarbon and perfluoroctylbromide, and a phosphoglyceride at a concentration of about 1-5%, and insulin; administering said emulsion to animals in amounts sufficient to cause said emulsion to contract said tumor cell mass and; visualizing said emulsion present in said tumor cell mass.
Claim 18
Independent Claim
83 words
A method for enhancing the detection of tumor cell mass in animals, comprising the steps of: forming an emulsion of fluorocarbon, said fluorocarbon being at a concentration of about 10-50%, said fluorocarbon being radiopaque and selected from the group consisting of perfluorocarbon and perfluoroctylbromide, and a phosphoglyceride at a concentration of about 1-5%, and insulin; administering said emulsion to animals in amounts sufficient to cause said emulsion to contract said tumor cell mass and; visualizing said emulsion present in said tumor cell mass.
Claim 19
Dependent Claim
24 words
A method as described in claim 13 wherein said insulin present in said emulsion is administered intravenously to said animals in an effective amount.
Claim 20
Dependent Claim
29 words
A method as described in claim 14 wherein said insulin is administered to animals in one or more amounts of about 10 micrograms to 1 mg/kg of body weight.
Claim 21
Dependent Claim
61 words
A contrast composition useful for contrasting tumor cell mass from normal tissue patients, comprising: a fluorocarbon selected from the group consiting of perfluorocarbon and perfluoroctylbromide, said fluorocarbon being radiopaque and present at a concentration of about 10-50%; insulin at a concentration of about 10 micrograms-1 mg per kilogram of patient body weight; and a phosphoglyceride at a concentration of about 1-5%.
Claim 22
Dependent Claim
61 words
A contrast composition useful for contrasting tumor cell mass from normal tissue patients, comprising: a fluorocarbon selected from the group consiting of perfluorocarbon and perfluoroctylbromide, said fluorocarbon being radiopaque and present at a concentration of about 10-50%; insulin at a concentration of about 10 micrograms-1 mg per kilogram of patient body weight; and a phosphoglyceride at a concentration of about 1-5%.
Claim 23
Dependent Claim
13 words
A composition as described in claim 16 wherein said phosphoglyceride is phosphatidyl choline.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1991-08-30 | 1992-02-29 | 1992-08-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-08-30 | 1996-02-29 | 1996-08-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-08-30 | 2000-02-29 | 2000-08-30 | $7700.00 | Window Closed / Lapsed |