US4764601A
Expired / Lapsed
US
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Composition for assaying cardiac glycosides from serum or plasma
Assignee: Ohio State University Research Foundation
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Filing: 1985-10-18
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Granted: 1988-08-16
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Expires: 2005-10-18
Abstract
Abstract A fluorescent compound is made from the reaction of naphthoyl chloride and a cardiac glycoside derived from serum or plasma. The method of carrying out the reaction provides a product which allows for a 100-fold increase in sensitivity when the product is subjected to chromatographic analysis according to the described apparatus.
Inventors
- 👤 Richard H. Reuning
- 👤 James Hui
- 👤 Theresa A. Shepard
- 👤 Richard A. Sams
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 17:46
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9453; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/20; Y10T436/203332; Y10T436/25
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
78 words
A composition consisting essentially of: (i) a first fluorescent compound which includes a moiety of a cardiac glycoside from one of serum and plasma derivatized by a naphthoyl moiety from a naphthoyl chloride purified by fractional distillation; (ii) a second and different flourescent compound which includes a moiety of a known cardiac glycoside derivatized by a naphthoyl moiety from said naphthoyl chloride purified by fractional distillation; and (iii) a liquid mobile phase for said first and second compounds.
Claim 2
Dependent Claim
78 words
A composition consisting essentially of: (i) a first fluorescent compound which includes a moiety of a cardiac glycoside from one of serum and plasma derivatized by a naphthoyl moiety from a naphthoyl chloride purified by fractional distillation; (ii) a second and different flourescent compound which includes a moiety of a known cardiac glycoside derivatized by a naphthoyl moiety from said naphthoyl chloride purified by fractional distillation; and (iii) a liquid mobile phase for said first and second compounds.
Claim 3
Dependent Claim
19 words
The composition of claim 1 in which the liquid mobile phase consists essentially of hexane, methylene chloride and acetonitrile.
Claim 4
Dependent Claim
20 words
The composition of claim 2 wherein the hexane, methylene chloride and acetonitrile are present in a ratio of about 27:5:5.
Claim 5
Dependent Claim
18 words
The composition of claim 1 in which digoxin is said cardiac glycoside from one of serum or plasma.
Claim 6
Dependent Claim
27 words
The composition of claim 1 in which said cardiac glycoside from one of serum or plasma is digoxin and in which said known cardiac glycoside is digitoxin.
Claim 7
Dependent Claim
17 words
The composition of claim 5 in which said naphthoyl chloride purified by fractional distillation is 1-naphthoyl chloride.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-08-16 | 1992-02-15 | 1992-08-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-08-16 | 1996-02-15 | 1996-08-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-08-16 | 2000-02-15 | 2000-08-16 | $7700.00 | Window Closed / Lapsed |