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US4764279A
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Process for preparing the principal proteins of hemolyzed blood in the non-denatured form
Assignee: Institut Merieux SA
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Filing: 1986-06-13
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Granted: 1988-08-16
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Expires: 2006-06-13
Abstract
Abstract The process comprises subjecting the hemolyzed blood to a clarification step at a pH of between 4 and 6 in the presence of less than 15% of alcohol, then, after concentration, effecting a chromatography by exchange of anions so as to separate the hemoglobin, the albumin being thereafter eluted. The hemoglobin and the globulins may then be separated out by precipitation with 25% alcohol at a pH of 7. The proteins obtained are in the native state.
Inventors
- 👤 Jean L. Tayot
- 👤 Paule A. Gattel
- 👤 Michel A. Tardy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 04:46
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/76; C07K14/765; C07K14/795; C07K14/805; C07K16/00; C07K16/06; C07K16/065; A; A61; A61K; A61K38/00; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25125
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
103 words
A process for separating hemoglobin, γ-globulins and albumin from hemolyzed blood, comprising subjecting said hemolyzed blood to a clarification step comprising contacting said hemolyzed blood at a pH of between 4 and 6 with alcohol at a concentration that does not exceed 15% and eliminating the precipitate which is obtained, then effecting an anion exchange chromatography of the clarified hemolyzed blood containing hemoglobin, γ-globulin and albumin in a chromatographic column on an anion exchanging chromatographic support at a pH higher than 4.8 and lower than 6.8, thereafter separately recovering hemoglobin including γ-globulins issuing from the chromatographic column as filtrate and albumin by elution.
Claim 2
Dependent Claim
103 words
A process for separating hemoglobin, γ-globulins and albumin from hemolyzed blood, comprising subjecting said hemolyzed blood to a clarification step comprising contacting said hemolyzed blood at a pH of between 4 and 6 with alcohol at a concentration that does not exceed 15% and eliminating the precipitate which is obtained, then effecting an anion exchange chromatography of the clarified hemolyzed blood containing hemoglobin, γ-globulin and albumin in a chromatographic column on an anion exchanging chromatographic support at a pH higher than 4.8 and lower than 6.8, thereafter separately recovering hemoglobin including γ-globulins issuing from the chromatographic column as filtrate and albumin by elution.
Claim 3
Dependent Claim
18 words
A process according to claim 1, comprising effecting the chromatography at a pH of between 5 and 6.
Claim 4
Dependent Claim
18 words
A process according to claim 1, comprising effecting the clarification at a pH of between 4.8 and 5.4.
Claim 5
Dependent Claim
22 words
A process according to claim 1, comprising effecting the clarification step in the presence of alcohol at a concentration of about 8%.
Claim 6
Dependent Claim
16 words
A process according to claim 1, comprising effecting the clarification step in the presence of ethanol.
Claim 7
Dependent Claim
14 words
A process according to claim 1, comprising separating the γ-globulins from the recovered hemoglobin.
Claim 8
Dependent Claim
26 words
A process according to claim 6, comprising separating the γ-globulins by precipitation with alcohol at about 25% and with a pH in the region of 7.
Claim 9
Dependent Claim
20 words
A process according to claim 1, comprising employing for the chromatography a support of porous silica coated with DEAE Dextran.
Claim 10
Dependent Claim
35 words
A process according to claim 1, comprising equilibrating the chromatographic column with an 0.01M phosphate buffer, then rinsing the column in the same buffer, the albumin being eluted with a 20 g/l solution of NaCl.
Claim 11
Dependent Claim
117 words
A process for separating hemoglobin and albumin from a hemolyzed blood from which γ-globulins have been separated by submitting said hemolyzed blood to an alcoholic γ-globulins separation step comprising subjecting said hemolyzed blood to a clarification step which comprises diluting said hemolyzed blood to an alcohol content that does not exceed 15%, at a pH of between 4 and 6 and eliminating the precipitate which is obtained, then effecting an anion exchange chromatography of the clarified hemolyzed blood containing hemoglobin and albumin in a chromatographic column on an anion exchanging chromatographic support at a pH higher than 4.8 and lower than 6.8, thereafter separately recovering hemoglobin issuing from the chromatographic column as filtrate and albumin by elution.
Claim 12
Dependent Claim
117 words
A process for separating hemoglobin and albumin from a hemolyzed blood from which γ-globulins have been separated by submitting said hemolyzed blood to an alcoholic γ-globulins separation step comprising subjecting said hemolyzed blood to a clarification step which comprises diluting said hemolyzed blood to an alcohol content that does not exceed 15%, at a pH of between 4 and 6 and eliminating the precipitate which is obtained, then effecting an anion exchange chromatography of the clarified hemolyzed blood containing hemoglobin and albumin in a chromatographic column on an anion exchanging chromatographic support at a pH higher than 4.8 and lower than 6.8, thereafter separately recovering hemoglobin issuing from the chromatographic column as filtrate and albumin by elution.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-08-16 | 1992-02-15 | 1992-08-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-08-16 | 1996-02-15 | 1996-08-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-08-16 | 2000-02-15 | 2000-08-16 | $7700.00 | Window Closed / Lapsed |