US4762788A
Expired / Lapsed
US
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Process for producing cellulolytic enzymes
Assignee: IFP Energies nouvelles
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Filing: 1984-11-28
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Granted: 1988-08-09
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Expires: 2004-11-28
Abstract
Abstract This invention concerns a process of producing cellulolytic enzymes by fermentation with the Trichoderma reesei fungus. This process includes two steps: (a) a first step of subjecting to aerobian fermentation a culture medium comprising a strain of Trichoderma reesei, nutrition compounds and a small quantity of cellulose and sugar, (b) a second step of contining the aerobian fermentation by adding soluble sugar at a speed such that the sugar concentration in the fermentation zone remains under 0.3% by weight. The cellulolytic enzymes obtained are used in the enzymatic hydrolysis of a lignocellulosic biomass.
Inventors
- 👤 Michel Warzywoda
- 👤 Veronique Ferre
- 👤 Jacques Pourquie
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 09:12
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2437; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/945
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
180 words
A process for aerobic production of cellulase by cultivation of a Trichoderma reesei strain in a fermentation zone containing a nutrient medium, said process comprising feeding said fermentation zone with soluble sugar and being characterized by: (a) a first step of subjecting to aerobic fermentation conditions a culture medium comprising a Trichoderma reesei strain, an inorganic salt and an initial carbon substrate containing at least one cellulose containing material and at least one soluble sugar, the amount by weight of said cellulose and said soluble sugar, in proportion to the culture medium, being each 0.01-2%, the first step being continued without further soluble sugar addition for a sufficient time to consume at least 10% of the sugar and to obtain a sugar concentration in the medium lower than 0.3% by weight, and (b) a second step of continuing the aerobic fermentation by adding soluble sugar continuously so as to introduce a sugar amount totalling at least 3% by weight of the culture medium while maintaining the sugar concentration in said culture medium at a value lower than 0.3% by weight.
Claim 2
Dependent Claim
180 words
A process for aerobic production of cellulase by cultivation of a Trichoderma reesei strain in a fermentation zone containing a nutrient medium, said process comprising feeding said fermentation zone with soluble sugar and being characterized by: (a) a first step of subjecting to aerobic fermentation conditions a culture medium comprising a Trichoderma reesei strain, an inorganic salt and an initial carbon substrate containing at least one cellulose containing material and at least one soluble sugar, the amount by weight of said cellulose and said soluble sugar, in proportion to the culture medium, being each 0.01-2%, the first step being continued without further soluble sugar addition for a sufficient time to consume at least 10% of the sugar and to obtain a sugar concentration in the medium lower than 0.3% by weight, and (b) a second step of continuing the aerobic fermentation by adding soluble sugar continuously so as to introduce a sugar amount totalling at least 3% by weight of the culture medium while maintaining the sugar concentration in said culture medium at a value lower than 0.3% by weight.
Claim 3
Dependent Claim
16 words
A process according to claim 1, wherein no cellulose-containing material is added during the second step.
Claim 4
Dependent Claim
20 words
A process according to claim 1, wherein the soluble sugar is lactose, glucose, xylose, arabinose, cellobiose or a mixture thereof.
Claim 5
Dependent Claim
23 words
A process according to claim 1, wherein the fermentation zone is fed with soluble sugar during the second step in a continuous manner.
Claim 6
Dependent Claim
13 words
A process according to claim 1, wherein the cellulose-containing material is paper pulp.
Claim 7
Dependent Claim
47 words
A process according to claim 1, wherein in step (a) the cellulose and sugar amounts are respectively 0.2-2% and 0.02-1% by weight and the contact is maintained until the sugar concentration becomes lower than 0.2% by weight and at least 20% of the sugar has been consumed.
Claim 8
Dependent Claim
33 words
A process according to claim 1, wherein the sugar 30 amount in step (a) is 10-50% by weight of the cellulose amount and the sugar concentration during step (b) is 0.1-0.25% by weight.
Claim 9
Dependent Claim
12 words
A process according to claim 3, wherein the soluble sugar is lactose.
Claim 10
Dependent Claim
25 words
A process according to claim 1, wherein the concentration of sugar in step (b) is maintained at a value of 0.1 to 0.3% by weight.
Claim 11
Dependent Claim
14 words
A process according to claim 1, wherein a hyperproducing Trichoderma reesei strain is used.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-08-09 | 1992-02-08 | 1992-08-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-08-09 | 1996-02-08 | 1996-08-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-08-09 | 2000-02-08 | 2000-08-09 | $7700.00 | Window Closed / Lapsed |