US4762701A
Expired / Lapsed
US
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In vivo cellular tracking
Assignee: SKB AND ZYNAXIS CELL SCIENCE Inc A CORP OF PENNSYLVANIA
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Filing: 1986-10-31
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Granted: 1988-08-09
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Expires: 2006-10-31
Abstract
Abstract Methods of tracking cells in vivo and for determining in vivo cell lifetimes. Cells are labelled with cyanine dyes and detection is by measuring fluorescence, absorbance, or by detecting nuclear magnetic resonance probes included in the cyanine dyes. Using the invented methods, for example, red blood cell and platelet lifetimes are determined. Also, cells are tracked to determine sites of primary or metastatic tumors, or sites of occult infection. Further, rates at which cells pass through vessels is used to determine blood vessel patency and platelet aggregation.
Inventors
- 👤 Paul K. Horan
- 👤 Sue E. Slezak
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 13:01
CPC Classifications
A; A61; A61K; A61K49/00; A61K49/001; A61K49/0013; A61K49/0017; A61K49/0019; A61K49/0021; A61K49/0032; A61K49/005; A61K49/0058; A61K49/0063; A61K49/0069; A61K49/0097
Claims Analysis
Total Claims: 43
Claim 1
Independent Claim
24 words
A method for tracking cells in vivo in a subject that comprises determining the location of cyanine dye-labelled cells previously administered to the subject.
Claim 2
Independent Claim
24 words
A method for tracking cells in vivo in a subject that comprises determining the location of cyanine dye-labelled cells previously administered to the subject.
Claim 3
Dependent Claim
15 words
A method of claim 1 wherein the cyanine dye has a nuclear magnetic resonance probe.
Claim 4
Dependent Claim
22 words
A method of claim 1 wherein the cyanine dye-labelled cells are tumor cell-seeking so that primary or metastatic tumor cells are detected.
Claim 5
Dependent Claim
15 words
A method of claim 3 wherein the cyanine dye-labelled cells are lymphocytes or natural-killer cells.
Claim 6
Dependent Claim
19 words
A method of claim 4 wherein the lymphocytes or natural-killer cells are activated prior to administration to the subject.
Claim 7
Dependent Claim
14 words
A method of claim 3 wherein the tumor cell-seeking cyanine dye-labelled cells are monocytes.
Claim 8
Dependent Claim
28 words
A method of claim 3 wherein the tumor cell-seeking cells are monocytes having tumor specific monoclonal antibodies bound to the monocytes so that tumor cell recognition is maintained.
Claim 9
Dependent Claim
14 words
A method of claim 3 wherein the tumor cell-seeking cyanine dye-labelled cells are platelets.
Claim 10
Dependent Claim
14 words
A method of claim 3 wherein the tumor cell-seeking cyanine dye-labelled cells are neutrophils.
Claim 11
Dependent Claim
17 words
A method of claim 1 wherein the cyanine dye is DISC 14 (5) or DIOC 14 (3).
Claim 12
Dependent Claim
15 words
A method of claim 10 wherein the cyanine dye is tagged with a gamma emitter.
Claim 13
Dependent Claim
17 words
A method of claim 10 wherein the cyanine dye is tagged with a nuclear magnetic resonance probe.
Claim 14
Dependent Claim
25 words
A method of claim 1 wherein the cyanine dye-labelled cells specifically interact with an organism infecting the subject so that the infection site is determined.
Claim 15
Dependent Claim
13 words
A method of claim 13 wherein the organism is a bacterium or fungus.
Claim 16
Dependent Claim
12 words
A method of claim 14 wherein the cyanine dye-labelled cells are neutrophils.
Claim 17
Dependent Claim
14 words
A method of claim 1 wherein the cyanine dye-labelled cells are red blood cells.
Claim 18
Dependent Claim
26 words
A method of claim 16 wherein the subject's retina is examined for presence of cyanine dye-labelled red blood cells to detect retinopathv or blood vessel degeneration.
Claim 19
Dependent Claim
22 words
A method of claim 16 wherein the rate at which the cyanine dye-labelled red blood cells flow through blood vessels is measured.
Claim 20
Dependent Claim
19 words
A method of claim 16 wherein the excitation wavelength of the cyanine dye is outside the human visable spectrum.
Claim 21
Dependent Claim
12 words
A method of claim 1 wherein the cyanine dye-labelled cells are platelets.
Claim 22
Dependent Claim
17 words
A method of claim 20 wherein aggregates of cvanine dye-labelled platelets are detected in retinal blood vessels.
Claim 23
Dependent Claim
16 words
A method of claim 1 wherein the cyanine dye-labelled cells are monocytes, neutrophils, lymphocytes, or platelets.
Claim 24
Dependent Claim
15 words
A method of claim 22 wherein the cyanine dye is tagged with a gamma emitter.
Claim 25
Dependent Claim
18 words
A method of claim 23 wherein the cyanine dye-labelled neutrophils are used to detect the location of infection.
Claim 26
Dependent Claim
18 words
A method of claim 23 wherein cyanine dye-labelled cells are used with imaging methods to determine arterial constriction.
Claim 27
Dependent Claim
17 words
A method of claim 23 wherein the cyanine dye-labelled cells are used in the staging of arthritis.
Claim 28
Dependent Claim
18 words
A method of claim 23 wherein the cyanine dye-labelled cells are use to monitor transplant or organ rejection.
Claim 29
Independent Claim
29 words
A method for determining in vivo cell lifetime in a subject that comprises measuring the rate at which cyanine dye-labelled cells administered to the subject disappear from said subject.
Claim 30
Independent Claim
29 words
A method for determining in vivo cell lifetime in a subject that comprises measuring the rate at which cyanine dye-labelled cells administered to the subject disappear from said subject.
Claim 31
Dependent Claim
17 words
A method of claim 28 wherein the cyanine dye-labelled cells are used in the staging of arthritis.
Claim 32
Dependent Claim
14 words
A method of claim 28 wherein the cyanine dye-labelled cells are red blood cells.
Claim 33
Dependent Claim
34 words
A method of claim 30 wherein the rate of disappearance of the cyanine dye-labelled red blood cells is compared to hematocrit changes to distinguish between bleeding and immunologic reaction to the red blood cells.
Claim 34
Dependent Claim
27 words
A method of claim 30 wherein total blood volume is determined by injecting a fixed number of completely labelled cells and measuring the dilution factor after equilibration.
Claim 35
Dependent Claim
19 words
A method of claim 30 wherein the cyanine dye-labelled red blood cells are prepared by labelling the subject's cells.
Claim 36
Dependent Claim
23 words
A method of claim 30 wherein the cyanine dye-labelled red blood cells are prepared by labelling random donor type O red blood cells.
Claim 37
Dependent Claim
12 words
A method of claim 28 wherein the cyanine dye-labelled cells are platelets.
Claim 38
Dependent Claim
35 words
A method of claim 35 wherein the rate of disappearance of the cyanine dye-labelled platelets is compared to total platelet counts to distinguish between bleeding or decreased platelet production and immunologic reaction to the platelets.
Claim 39
Dependent Claim
19 words
A method of claim 35 where the lifetime of cyanine dye-labelled platelets is determined as a measure of atherosclerosis.
Claim 40
Dependent Claim
17 words
A method of claim 28 wherein the cyanine dye is DiSC 14 (5) or DiOC 14 (3).
Claim 41
Dependent Claim
15 words
A method of claim 38 wherein cyanine dye-labelled platelets are measured intraocularly to detect microemboli.
Claim 42
Independent Claim
39 words
A method for tracking cells in vivo in a subject that comprises: obtaining cells to be cyanine dye-labelled, labelling the cells with a cyanine dye, administering the cyanine dye-labelled cells to the subject, and detecting the cyanine dye-labelled cells.
Claim 43
Independent Claim
39 words
A method for tracking cells in vivo in a subject that comprises: obtaining cells to be cyanine dye-labelled, labelling the cells with a cyanine dye, administering the cyanine dye-labelled cells to the subject, and detecting the cyanine dye-labelled cells.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-08-09 | 1992-02-08 | 1992-08-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-08-09 | 1996-02-08 | 1996-08-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-08-09 | 2000-02-08 | 2000-08-09 | $7700.00 | Window Closed / Lapsed |