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US4760020A
Expired / Lapsed
US
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Method for testing biocompatibility
Assignee: The Schepens Eye Research Institute Inc
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Filing: 1985-05-03
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Granted: 1988-07-26
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Expires: 2005-05-03
Abstract
Abstract An in vitro testing method for biocompatibility, particularly toxicological effect, of substances is based on the change in the time for closure of a disruption in a confluent cell culture caused by treatment with the substance. The method can be used to replace a substantial number of toxicity tests, including the Draize test for ocular irritancy.
Inventors
- 👤 Arthur H. Neufeld
- 👤 Marcia M. Jumblatt
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 21:00
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5008; G01N33/5014
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
88 words
A method of testing a substance for toxicological effect on a specified mammalian tissue, said method comprising the steps of preparing a culture of confluent cells, producing a selected and repeatable disruption in said confluent cell culture, treating said disrupted confluent cell culture with the substance to be tested for toxicological effect, incubating said treated cell culture to affect closure of said disruption, and determining the toxicological effect of said substance by comparing the relative degree of closure of said disruption with standards of disrupted confluent cell cultures.
Claim 2
Independent Claim
88 words
A method of testing a substance for toxicological effect on a specified mammalian tissue, said method comprising the steps of preparing a culture of confluent cells, producing a selected and repeatable disruption in said confluent cell culture, treating said disrupted confluent cell culture with the substance to be tested for toxicological effect, incubating said treated cell culture to affect closure of said disruption, and determining the toxicological effect of said substance by comparing the relative degree of closure of said disruption with standards of disrupted confluent cell cultures.
Claim 3
Dependent Claim
14 words
A method according to claim 1 wherein said specified mammalian tissue comprises ocular tissue.
Claim 4
Dependent Claim
40 words
A method according to claim 1 wherein said standards comprise cultures of said confluent cells which are not treated with said material, said untreated cell cultures being disrupted and grown in a substantially identical manner to said treated cell culture.
Claim 5
Dependent Claim
18 words
A method according to claim 1 wherein said culture of confluent cells in said step comprises epithelial cells.
Claim 6
Dependent Claim
15 words
A method according to claim 1 wherein said culture of confluent cells comprises mammalian cells.
Claim 7
Dependent Claim
15 words
A method according to claim 5 wherein said confluent mammalian cells comprise corneal epithelial cells.
Claim 8
Dependent Claim
27 words
A method according to claim 6 wherein said corneal epithelial cells are selected from a group consisting of rabbit corneal epithelial cells and human corneal epithelial cells.
Claim 9
Dependent Claim
38 words
A method according to claim 1 wherein said step of determining toxicological effect of said substance comprises testing a plurality of amounts of said substance to determine an amount at which said substance inhibits closure of said disruption.
Claim 10
Dependent Claim
21 words
A method according to claim 8 wherein said determining step comprises testing serial dilutions of said substance to determine said amount.
Claim 11
Dependent Claim
28 words
A method according to claim 1 wherein said disruption producing step includes the step of producing a disruption having a specific size and shape in said cell culture.
Claim 12
Dependent Claim
25 words
A method according to claim 10 wherein said disruption producing step comprises the step of inflicting a specified freezing condition upon said size-delineated disruption area.
Claim 13
Dependent Claim
23 words
A method according to claim 9 wherein said freezing step comprises lowering the temperature of said area to the temperature of liquid nitrogen.
Claim 14
Dependent Claim
24 words
A method according to claim 11 wherein said disruption producing step includes contacting said cell culture with a probe having a selected sub-freezing temperature.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-07-26 | 1992-01-25 | 1992-07-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-07-26 | 1996-01-25 | 1996-07-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-07-26 | 2000-01-25 | 2000-07-26 | $7700.00 | Window Closed / Lapsed |